Structures of polysaccharides and oligosaccharides of some Gram-negative marine Proteobacteria

Nazarenko, Evgeny L.; Komandrova, Nadezhda A.; Gorshkova, Raisa P.; Tomshich, Svetlana V.; Зубков, В. И.; Kilcoyne, Michelle; Savage, Angela V.

doi:10.1016/j.carres.2003.06.004

Cited by 60 publications

(38 citation statements)

References 48 publications

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“…Moreover, the OPSs from marine bacteria LPSs often contain higher (up to 10 carbon atoms) or peculiar (presence of deoxy, amino and carboxyl functions; ester, ether and amide groups) monosaccharides, as well as non-carbohydrate appendages (i.e., phosphate or sulphate groups, polyols, carboxylic and amino acids. Marine bacteria polysaccharides have been previously reviewed by Nazarenko et al [9]). …”

Section: Lps Molecular Architecture and Activitymentioning

confidence: 99%

Molecular Structure of Endotoxins from Gram-negative Marine Bacteria: An Update

Leone

2007

Mar. Drugs

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Marine bacteria are microrganisms that have adapted, through millions of years, to survival in environments often characterized by one or more extreme physical or chemical parameters, namely pressure, temperature and salinity. The main interest in the research on marine bacteria is due to their ability to produce several biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents. Nonetheless, lipopolysaccharides (LPSs), or their portions, from Gram-negative marine bacteria, have often shown low virulence, and represent potential candidates in the development of drugs to prevent septic shock. Besides, the molecular architecture of such molecules is related to the possibility of thriving in marine habitats, shielding the cell from the disrupting action of natural stress factors. Over the last few years, the depiction of a variety of structures of lipids A, core oligosaccharides and O-specific polysaccharides from LPSs of marine microrganisms has been given. In particular, here we will examine the most recently encountered structures for bacteria belonging to the genera Shewanella, Pseudoalteromonas and Alteromonas, of the γ-Proteobacteria phylum, and to the genera Flavobacterium, Cellulophaga, Arenibacter and Chryseobacterium, of the CytophagaFlavobacterium-Bacteroides phylum. Particular attention will be paid to the chemical features expressed by these structures (characteristic monosaccharides, non-glycidic appendages, phosphate groups), to the typifying traits of LPSs from marine bacteria and to

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Section: Lps Molecular Architecture and Activitymentioning

confidence: 99%

Molecular Structure of Endotoxins from Gram-negative Marine Bacteria: An Update

Leone

2007

Mar. Drugs

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“…However, although a number of polysaccharides and oligosaccharides from various species of Pseudoalteromonas have been chemically and structurally characterized to reveal the presence of unusual sugars, including those with uncommon nonsugar substituents (49), to our knowledge, this is the first report describing an exopolysaccharide (EPS) from a Pseudoalteromonas species containing xylose at significantly high levels. Other studies describing EPS from Pseudoalteromonas species have reported xylose as a minor component (Ͻ1% of total carbohydrate) or as absent altogether (46,49,63). As xylose is rarely found in bacterial EPS (35), its high abundance in PE12 is an interesting finding whose significance remains unclear to us at present.…”

Section: Discussionmentioning

confidence: 98%

“…Its high uronic acid (28.7%) and low protein (8.2%) contents are chemical features that have been reported in exopolymers derived from other Pseudoalteromonas/Alteromonas species (14,44,55,62). However, although a number of polysaccharides and oligosaccharides from various species of Pseudoalteromonas have been chemically and structurally characterized to reveal the presence of unusual sugars, including those with uncommon nonsugar substituents (49), to our knowledge, this is the first report describing an exopolysaccharide (EPS) from a Pseudoalteromonas species containing xylose at significantly high levels. Other studies describing EPS from Pseudoalteromonas species have reported xylose as a minor component (Ͻ1% of total carbohydrate) or as absent altogether (46,49,63).…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, members within the genus Pseudoalteromonas have been recognized for producing exopolymers with properties of biotechnological interest. Although the chemical and physical properties of various Pseudoalteromonas exopolymers have been described (11,44,46,49), to our knowledge no reports exist that describe their emulsifying activities or emulsion-stabilizing properties. There is limited information on the metal-binding properties of exopolymers produced by pseudoalteromonad species (39).…”

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confidence: 99%

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Emulsifying and Metal Ion Binding Activity of a Glycoprotein Exopolymer Produced byPseudoalteromonassp. Strain TG12

Gutiérrez¹,

Shimmield²,

Haidon³

et al. 2008

Appl Environ Microbiol

103

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In this study, we describe the isolation and characterization of a new exopolymer that exhibits high emulsifying activities against a range of oil substrates and demonstrates a differential capacity to desorb various mono-, di-, and trivalent metal species from marine sediment under nonionic and seawater ionicstrength conditions. This polymer, PE12, was produced by a new isolate, Pseudoalteromonas sp. strain TG12 (accession number EF685033), during growth in a modified Zobell's 2216 medium amended with 1% glucose. Chemical and chromatographic analysis showed it to be a high-molecular-mass (>2,000 kDa) glycoprotein composed of carbohydrate (32.3%) and protein (8.2%). PE12 was notable in that it contained xylose as the major sugar component at unusually high levels (27.7%) not previously reported for a Pseudoalteromonas exopolymer. The polymer was shown to desorb various metal species from marine sediment-a function putatively conferred by its high content of uronic acids (28.7%). Seawater ionic strength (simulated using 0.6 M NaCl), however, caused a significant reduction in PE12's ability to desorb the sediment-adsorbed metals. These results demonstrate the importance of electrolytes, a physical parameter intrinsic of seawater, in influencing the interaction of microbial exopolymers with metal ions. In summary, PE12 may represent a new class of Pseudoalteromonas exopolymer with a potential for use in biotechnological applications as an emulsifying or metal-chelating agent. In addition to the biotechnological potential of these findings, the ecological aspects of this and related bacterial exopolymers in marine environments are also discussed.

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“…According with literature (34), the polysaccharides isolated from various Gram-negative bacteria (belonging to Pseudoalteromonas and Shewanella genera) are distinguished by the acidic character (e.g., due to the presence of hexuronic and aldulosonic acids and their derivatives) and the occurrence of unusual sugars, including n-acyl derivatives of 6-deoxyamino sugars, such as nacetyl-D-quinovosamine, n-acetyl-l-fucosamine and n-acetyl-6-deoxy-l-talosamine, and higher sugars like 2,6-dideoxy-2-acetamido-4-c-(3'-carboxamide-2',2'-dihydroxypropyl)-D-galactopyranose (shewanellose). Many constituent sugars have various uncommon non-sugar substituents, such as alanine, formic, lactic and hydroxybutyric acids, sulfate, phosphate, and 2-aminopropane-1,3-diol (34).…”

Section: Specific Amplification Of Hae1 (Hydrophobe/amphiphile Effluxmentioning

confidence: 99%

Adaptative Response ofShewanella PutrefaciensandPseudomonas Aeruginosato Toxic Organic Solvents

Lazaroaie¹

2010

Biotechnology & Biotechnological Equipment

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Structures of polysaccharides and oligosaccharides of some Gram-negative marine Proteobacteria

Cited by 60 publications

References 48 publications

Molecular Structure of Endotoxins from Gram-negative Marine Bacteria: An Update

Molecular Structure of Endotoxins from Gram-negative Marine Bacteria: An Update

Emulsifying and Metal Ion Binding Activity of a Glycoprotein Exopolymer Produced byPseudoalteromonassp. Strain TG12

Adaptative Response ofShewanella PutrefaciensandPseudomonas Aeruginosato Toxic Organic Solvents

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