Structures of organic additives modified magnetite nanoparticles

Kuwahara, Yoshimitsu; Miyazaki, Toshiki; Shirosaki, Yuki; Liu, Gengci; Kawashita, Masakazu

doi:10.1016/j.ceramint.2015.12.152

Cited by 13 publications

(13 citation statements)

References 14 publications

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“…bDDM-control, bLDAO-control, and bTriton-control experiments show a clear influence of the Tris buffer on magnetite precipitation. Such an effect has been pointed out before by Kuwahara et al, who demonstrated that Tris buffer (4 mass %) in an Fe 3+ /Fe 2+ solution prevents magnetite nucleation, giving rise to akaganaeite formation instead. This is caused by the complexation of iron cations with the amino groups of Tris.…”

Section: Discussionmentioning

confidence: 66%

Magnetite Nanoparticles Biomineralization in the Presence of the Magnetosome Membrane Protein MamC: Effect of Protein Aggregation and Protein Structure on Magnetite Formation

López-Moreno¹,

Fernández‐Vivas²,

Valverde-Tercedor³

et al. 2017

Crystal Growth & Design

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MamC from Magnetococcus marinus MC-1 has been shown to control the size of magnetite crystals in in vitro experiments, thereby demonstrating its potential as a candidate protein for the production of magnetite nanoparticles possibly useful in medical and other applications. However, the importance of the structure and aggregation state of the protein on the resulting biomimetic nanoparticles has not yet been assessed. One method normally used to prevent the aggregation of integral membrane proteins is the introduction of detergents during protein purification. In this study, results from protein aggregation following the addition of Triton-X100, DDM, and LDAO are presented. Magnetite particles formed in the presence of MamC purified using these three detergents were compared. Our results show that detergents alter the structure of the folded recombinant protein, thus preventing the ability of MamC to control the size of magnetite crystals formed chemically in vitro. Furthermore, we show that the introduction of detergents only at the dialysis process during the protein purification prevents its aggregation and allows for correct, functional folding of MamC. These results also indicate that the population of the active protein particles present at a certain oligomeric state needs to be considered, rather than only the oligomeric state, in order to interpret the ability of magnetosome recombinant proteins to control the size and/or morphology of magnetite crystals formed chemically in vitro.

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Section: Discussionmentioning

confidence: 66%

Magnetite Nanoparticles Biomineralization in the Presence of the Magnetosome Membrane Protein MamC: Effect of Protein Aggregation and Protein Structure on Magnetite Formation

López-Moreno¹,

Fernández‐Vivas²,

Valverde-Tercedor³

et al. 2017

Crystal Growth & Design

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“…The use of additives in a green RTCP is an established method to offer control over nanoparticle synthesis. 25 Grafted co-polymer additives have been used to control the size of magnetite MNPs 26 while there are many examples of green/natural additive being used such as unrefined seaweed 27 and plant leaf extracts in the synthesis of MNPs, [28][29][30] as well as citrate, 31 oxalic acid 32 and chitosan 33 . Green additives such as fruit juice and sea buckthorn have also been used to control the size of gold 34 and silver 35,36 nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Ethylenediamine series as additives to control the morphology of magnetite nanoparticles

et al. 2021

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“…Increasing the amount of tartaric acid could favor the predominant formation of iron-complex instead of the stabilized magnetic iron oxide[30]. Other authors have reported similar observations, where the presence of analogous acids, such as oxalic in almost similar experimental conditions, rendered lepidocrosite or akageneite instead of magnetite [31]…”

mentioning

confidence: 63%

Selective contrast agents with potential to the earlier detection of tumors: Insights on synthetic pathways, physicochemical properties and performance in MRI assays

Schneider

Martín

Coral

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Magnetic iron oxide nanoparticles (MNPs) have been prepared and stabilized with three organic acids (tartaric, malic and ascorbic) in order to obtain biocompatible and water dispersible MNPs with potential to bind specifically to tumoral cancer cells. An in deep characterization was performed aiming to verify the presence and effect of the coating and stabilizer on MNPs surface. Besides the mechanisms followed by the different acids to bind MNPs were elucidated and used to justify the differences in the physicochemical properties of each formulation. Data related to characterization revealed that MNPs coated with ascorbic acid (MNPs-AA) resulted the most suitable in terms of their size, surface charge and stability along the time. Besides, ascorbic acid may be recognized by GLUTs receptors that are overexpressed in several kinds of tumoral cells. Therefore, MNPs-AA was selected to explore its performance in both MRI and in vitro assays using human colon cancer cells HCT 116. MRI experiments were performed in clinical equipment using a series of aqueous dispersions of MNPs-AA that were evaluated as T contrast agent. The T- weighted images obtained as well as the calculated r2, indicated that MNPs-AA could act as efficient T contrast agent for MRI. Regarding in vitro assays, MNPs-AA did not alter the cellular function neither exert cytotoxicity using the three explored doses. The internalization of the nanoparticles on the cellular structure was confirmed quanti and qualitatively using atomic absorption spectroscopy and Prussian blue techniques respectively. From these results, it emerges that ascorbic acid coated-magnetite nanoparticles may be used as alternative contrast agent to avoid or minimize some toxicological issues related to the widely used gadolinium.

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Structures of organic additives modified magnetite nanoparticles

Cited by 13 publications

References 14 publications

Magnetite Nanoparticles Biomineralization in the Presence of the Magnetosome Membrane Protein MamC: Effect of Protein Aggregation and Protein Structure on Magnetite Formation

Magnetite Nanoparticles Biomineralization in the Presence of the Magnetosome Membrane Protein MamC: Effect of Protein Aggregation and Protein Structure on Magnetite Formation

Ethylenediamine series as additives to control the morphology of magnetite nanoparticles

Selective contrast agents with potential to the earlier detection of tumors: Insights on synthetic pathways, physicochemical properties and performance in MRI assays

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