Structures of mollusc shell framework proteins

Sudo, Shu; Fujikawa, Takahiko; Nagakura, Takeshi; Ohkubo, Tadayasu; Sakaguchi, Kesami; Tanaka, Minoru; Nakashima, Kunio; Takahashi, Tsuyoshi

doi:10.1038/42391

Cited by 421 publications

(324 citation statements)

References 2 publications

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“…The first three constitute almost half of the total amino acids. This result is consistent with those previously obtained with other Nautilus species, [31] because the AIM exhibits the signature of hydrophobic extracts, which characterizes the "silk fibroin-like" proteins [10,27] found in the nacre of various mollusks. [37,39,40] The composition of the ASM is characterized by large amounts of Gly and Asx, which represent more than two-fifths of the total amino acids.…”

Section: Amino Acid Compositionssupporting

confidence: 92%

“…The AIM is enriched in aliphatic residues, mainly Gly and Ala, and in glucosamine. Gly and Ala are characteristic of "framework" proteins, [10] whereas large amounts of glucosamine indicate the likely presence of chitin. [29] The ASM is more hydrophilic, and enriched in Asx residues, whereas its sugar composition is shared out between different monosaccharide residues.…”

Section: Discussionmentioning

confidence: 99%

“…[5,6] Recent ultrastructural advances have shown that each nacre tablet has a hierarchical organization [7] and is composed of nanodomains. [8] In parallel, biochemical characterizations have shown that the nacre matrix comprises a large set of macromolecular components, such as chitin, [9] hydrophobic "framework" proteins, [10] proteins with elastomeric properties, [11] and several soluble proteins and glycoproteins. [12,13] For a few years, new dynamic models of nacre formation have been used in attempts to conciliate both biochemical and ultrastructural data.…”

Section: Introductionmentioning

confidence: 99%

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Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

Marie¹,

Marin²,

Marie³

et al. 2009

ChemBioChem

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Matrix evolutions: We have biochemically characterized the nacre matrix of the cephalopod Nautilus macromphalus, in part by a proteomic approach applied to the acetic acid‐soluble and ‐insoluble shell matrices, as well as to spots obtained after 2D gel electrophoresis. Strikingly, most of the obtained partial sequences are entirely new, whereas a few correspond only partly with bivalvian nacre proteins. Our findings shed new light on the macroevolution of nacre matrix proteins. magnified imageIn mollusks, one of the most widely studied shell textures is nacre, the lustrous aragonitic layer that constitutes the internal components of the shells of several bivalves, a few gastropods, and one cephalopod: the nautilus. Nacre contains a minor organic fraction, which displays a wide range of functions in relation to the biomineralization process. Here, we have biochemically characterized the nacre matrix of the cephalopod Nautilus macromphalus. The acid‐soluble matrix contains a mixture of polydisperse and discrete proteins and glycoproteins, which interact with the formation of calcite crystals. In addition, a few bind calcium ions. Furthermore, we have used a proteomic approach, which was applied to the acetic acid‐soluble and ‐insoluble shell matrices, as well as to spots obtained after 2D gel electrophoresis. Our data demonstrate that the insoluble and soluble matrices, although different in their bulk monosaccharide and amino acid compositions, contain numerous shared peptides. Strikingly, most of the obtained partial sequences are entirely new. A few only partly match with bivalvian nacre proteins. Our findings have implications for knowledge of the long‐term evolution of molluskan nacre matrices.

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Section: Amino Acid Compositionssupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

Marie¹,

Marin²,

Marie³

et al. 2009

ChemBioChem

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“…The primary structure, origin and evolution of shell matrix proteins of molluscs have been reviewed by Marin et al (2008). Oysters have two complex layers in their shells: the nacreous and the prismatic layers (Sudo et al 1997). Both layers are composed from microlaminate composites of calcium carbonate crystals (aragonite in the nacreous and calcite in the prismatic layer).…”

Section: Types Of Substrates and Mechanisms For Penetrationmentioning

confidence: 99%

The roles of endolithic fungi in bioerosion and disease in marine ecosystems. I. General concepts

et al. 2017

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Endolithic true fungi and fungus-like microorganisms penetrate calcareous substrates formed by living organisms, cause significant bioerosion and are involved in diseases of many host animals in marine ecosystems. A theoretical interactive model for the ecology of reef-building corals is proposed in this review. This model includes five principle partners that exist in a dynamic equilibrium: polyps of a colonial coelenterate, endosymbiotic zooxanthellae, endolithic algae (that penetrate coral skeletons), endolithic fungi (that attack the endolithic algae, the zooxanthellae and the polyps) and prokaryotic and eukaryotic microorganisms (which live in the coral mucus). Endolithic fungi and fungus-like boring microorganisms are important components of the marine calcium carbonate cycle because they actively contribute to the biodegradation of shells of animals composed of calcium carbonate and calcareous geological substrates.

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“…In the organic matrix of mollusk shells, chitin substrates with bound silk-fibroin-like proteins have an important role in regulating the morphology and lattice structure of the mineral phase (Sudo et al 1997;Sun and Bhushan 2012;Weiner and Traub 1980). Several of the attached proteins adopt antiparallel β-sheet conformations and consist of repetitive lowcomplexity domains (RLCDs) rich in glycine (Evans 2008;Jackson et al 2010).…”

Section: Mineralization and Liquid-like Phasesmentioning

confidence: 99%

Mineralization and non-ideality: on nature’s foundry

Rao¹,

Cölfen

2016

Biophys Rev

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Understanding how ions, ion-clusters and particles behave in non-ideal environments is a fundamental question concerning planetary to atomic scales. For biomineralization phenomena wherein diverse inorganic and organic ingredients are present in biological media, attributing biomaterial composition and structure to the chemistry of singular additives may not provide a holistic view of the underlying mechanisms. Therefore, in this review, we specifically address the consequences of physico-chemical non-ideality on mineral formation. Influences of different forms of non-ideality such as macromolecular crowding, confinement and liquid-like organic phases on mineral nucleation and crystallization in biological environments are presented. Novel prospects for the additive-controlled nucleation and crystallization are accessible from this biophysical view. In this manner, we show that non-ideal conditions significantly affect the form, structure and composition of biogenic and biomimetic minerals.

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Structures of mollusc shell framework proteins

Cited by 421 publications

References 2 publications

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the Cephalopod Nautilus macromphalus

The roles of endolithic fungi in bioerosion and disease in marine ecosystems. I. General concepts

Mineralization and non-ideality: on nature’s foundry

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