Structures of Acylated Anthocyan Pigments in Vitis vinifera Variety Tinta Pinheira. Identification of Anthocyanidin, Sugar, and Acid Moieties

Abstract

SUMMARY Investigation of the anthocyan skin pigments in hands 1, 2, and 3 isolated from Vitis vinifera var. Tinta Pinheira grapes showed that the pigments from hand 2 consisted of the 3‐mono‐glucosides of malvidin, peonidin, delphinidin, and petunidin, each acylated with p‐coumaric acid. The pigments from hand 3 consisted of the same four anthocyanins, each acylated with caffeic acid. Evidence is presented to show that the pigments in hand 1 were an artifact obtained under certain experimental conditions, and a tentative explanation for their formation is proposed.

Anthocyanins of the Garden Huckleberry, Solanum guineese

“…During the third purification in BAW, the pigment would often separate into two widely separated bands which upon subsequent purification would recombine. This was probably due to the acid-front phenomenon described by Albach et al (1965). Band 2 contained petunidin and malvidin derivatives which could not be separated by chromatography without excessive labor because even a fifth purification in BuHCl failed to separate the two.…”

Acylation of anthocyanins with hydroxycinnamic acids via 1-O-acylglucosides by protein preparations from cell cultures of Daucus carota L.

“…and hydrolyzed with 10% KOH according to Albach et al (1965). The pigments were extracted with methanol-acetic acidwater (50 : 8 : 42, by vol.)…”

Anthocyanin Pigments of Rhubarb, Canada Red

“…Molybdate spray reagent for pigments with vicinal phenolic groups was prepared as described by Albach et al (1965).…”

“…Partridge's reagent used for visualization of sugars on paper chromatograms was formulated as described by Albach et al (1965). Knowns man DK-1 spectrophotometer using 0.01% HCl in spectra grade methanol as a solvent.…”