Structures and mechanisms of formation of liprotides

Pedersen, Jannik Nedergaard; Otzen, Daniel E.

doi:10.1016/j.bbapap.2020.140505

Cited by 8 publications

(3 citation statements)

References 110 publications

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“…The prepared liprotides were further characterized for their size distribution and zeta potential, and results are summarized in Table 2. The reported diameter of a generic core-shell liprotide is ~100 Å or around 10 nm (Frislev et al, 2017), while multilayered liprotides are reported to be between 20 -30 nm (Pedersen et al, 2020). The measured diameter of the liprotides prepared in this work were significantly larger (658.7 -1946 nm), which may be primarily attributed to the crude nature of the proteins used as evidenced by the already large sizes for the uncomplexed protein controls, in contrast with the highly purified proteins utilized in previous studies.…”

Section: Resultsmentioning

confidence: 99%

Preparation and characterization of liprotides prepared from protein extracts of mung beans (Vigna radiata (L.))

Tamayo,

David,

Espiritu

2024

Preprint

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In recent years, liprotides, which are protein-fatty acid complexes with core-shell structures, have proven to be promising drug carriers for cancer treatment. Due to their novel nature, there are limited studies surrounding liprotides, especially one synthesized from plant-based proteins. Thus, to explore new possibilities for liprotide synthesis, mung bean albumins and globulins were each extracted and combined with oleic acid (OA) at different temperatures (40 °C and 80 °C) to synthesize four variations of plant-based liprotides (Alp40, Alp80, Glp40, and Glp80). These, along with OA and protein controls, were characterized through ATR-FTIR spectroscopy, visual stability testing, particle size analysis, and zeta potential analysis. The IR spectra of the liprotides compared to the controls suggested that the OA molecule was encapsulated within the protein shell as intended. Peak shifts in characteristic absorption bands were also observed, indicating possible structural changes that may or may not be correlated to liprotide formation. Particle size analysis showed that the synthesized liprotides had significantly larger diameters than those indicated in published data and were highly polydispersed (PdI > 0.4). Lastly, zeta-potential analysis of the samples revealed that the oleic acid controls garnered a negative zeta-potential greater than -70 mV. This contrasts the zeta-potential values of both protein controls and liprotides which ranged between -23 mV and -33 mV.

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Section: Resultsmentioning

confidence: 99%

Preparation and characterization of liprotides prepared from protein extracts of mung beans (Vigna radiata (L.))

Tamayo,

David,

Espiritu

2024

Preprint

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“…This ability has been proven to make liprotide carriers of hydrophobic molecules in hydrophilic environments (Ismail & Csóka, 2017). Liprotides have a core-shell structure, which consists of a fatty acid core in the form of micelles and is covered by partially denatured proteins (Pedersen et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Oleic acid interacts with proteins in various ways. This causes variations in the oleic acid to protein ratio, which can affect the ratio (Pedersen et al, 2020). Protein function increases the solubility of fatty acids.…”

Section: Introductionmentioning

confidence: 99%

Determination of vitamin D3 encapsulated in liprotide β-lactoglobulin crosslink oleic acid by HPLC and SEM

Putri,

Anjani,

Fulyani

et al. 2023

Aceh. Nutri. J.

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Vitamin D is an unstable compound to light, heat, and certain chemicals, so it is easily degraded under various conditions. Therefore, instability is achieved through the encapsulation method using complex protein carrier compounds with fatty acids known as liprotide. This study aims to determine vitamin D3. Method, the determination of vitamin D3 was analyzed, including the determination of vitamin D3 standard eluents, calibration curve, and determining the retention time of vitamin D3 encapsulated in liprotide using HPLC (High-Performance Liquid Chromatography). Morphology of vitamin D3 encapsulated in liprotide using SEM (Scanning Electron Microscopy). The results showed that the standard vitamin D3 HPLC analysis using acetonitrile: methanol and acetonitrile: aquabidest obtained two peaks. Butanol: n-hexane eluent obtained one peak with a retention time of 2,170 minutes. The results of the standard calibration curve for vitamin D3 at a linearity value of R2 = 0,9997 and f(x) = 14,928x-117,930. The same retention time was obtained for vitamin D3 encapsulated in liprotide. Conclusion, an enlargement of the cubic structure, which occurred due to the encapsulation of vitamin D3 by the liprotide-forming micelles.

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Encapsulation of fucoxanthin in fatty acid-bovine serum albumin micelles to improve the stability, bioavailability, and bioefficacy

Liu

et al. 2022

Colloids and Surfaces B: Biointerfaces

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Structures and mechanisms of formation of liprotides

Cited by 8 publications

References 110 publications

Preparation and characterization of liprotides prepared from protein extracts of mung beans (Vigna radiata (L.))

Preparation and characterization of liprotides prepared from protein extracts of mung beans (Vigna radiata (L.))

Determination of vitamin D3 encapsulated in liprotide β-lactoglobulin crosslink oleic acid by HPLC and SEM

Encapsulation of fucoxanthin in fatty acid-bovine serum albumin micelles to improve the stability, bioavailability, and bioefficacy

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