Structures and biofilm inhibition activities of brominated furanones for Escherichia coli and Pseudomonas aeruginosa

Shetye, Gauri; Singh, Nischal; Gao, Xiang; Bandyopadhyay, Debjyoti; Yan, Aixin; Luk, Yan Yeung

doi:10.1039/c3md00059a

Cited by 26 publications

(19 citation statements)

References 38 publications

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“…Biofilm production was significantly inhibited only at the maximum tested concentration of 20 mg L À1 (p < 0.05) compared to the blank control, with an inhibition rate of 14%. This inhibition level was relatively lower compared to other reported results in which brominated furanone was supplied in suspension (Janssens et al, 2008;Shetye et al, 2013). This may be due to that the BBF coating was fabricated by simply dipping glass slides into the BBF solution, so the amount of BBF coated onto the surface was very small and its concentration in the bulk solution was also very low.…”

Section: Effect Of Bbf or Nafion On Biofilm Inhibitioncontrasting

confidence: 60%

“…A series of furanones have been reported to pose the potential of inhibiting biofilm formation through disrupting QS systems and therefore been regarded as QS inhibitors (Janssens et al, 2008;Shetye et al, 2013). In this study, a brominated furanone was chosen and its ability to inhibit biofilm formation was explored.…”

Section: Effect Of Bbf or Nafion On Biofilm Inhibitionmentioning

confidence: 99%

“…Brominated furanones (BFs), a class of secondary metabolites originally extracted from the red alga Delisea pulchra, are the mostly studied QSIs (Janssens et al, 2008). Natural or synthetic BFs have been demonstrated to interfere with the QS systems involving N-acylated homoserine lactones (AHLs) by competing with AHLs for the receptor proteins (Shetye et al, 2013). In addition, some kinds of BFs can inhibit biofilm formation by interfering with autoinducer-2 (AI-2) QS systems, which widely exist in Gram-negative and Gram-positive bacteria (Jang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Incorporation of brominated furanone into Nafion polymer enhanced anti-biofilm efficacy

Quan

2015

International Biodeterioration & Biodegradation

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Section: Effect Of Bbf or Nafion On Biofilm Inhibitioncontrasting

confidence: 60%

Section: Effect Of Bbf or Nafion On Biofilm Inhibitionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Incorporation of brominated furanone into Nafion polymer enhanced anti-biofilm efficacy

Quan

2015

International Biodeterioration & Biodegradation

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“…Brominated furanones do not only inhibit LuxS but also other proteins involved in AI-1 perception [108]. A recent study showed that analogs of brominated furanones, with attenuated toxicity, have interesting anti-biofilm properties against E. coli and P. aeruginosa [109]. …”

Section: Small Molecule Inhibitors Of Ai-2 Signalingmentioning

confidence: 99%

Small Molecule Inhibitors of AI-2 Signaling in Bacteria: State-of-the-Art and Future Perspectives for Anti-Quorum Sensing Agents

Guo

Gamby

Zheng

et al. 2013

IJMS

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Bacteria respond to different small molecules that are produced by other neighboring bacteria. These molecules, called autoinducers, are classified as intraspecies (i.e., molecules produced and perceived by the same bacterial species) or interspecies (molecules that are produced and sensed between different bacterial species). AI-2 has been proposed as an interspecies autoinducer and has been shown to regulate different bacterial physiology as well as affect virulence factor production and biofilm formation in some bacteria, including bacteria of clinical relevance. Several groups have embarked on the development of small molecules that could be used to perturb AI-2 signaling in bacteria, with the ultimate goal that these molecules could be used to inhibit bacterial virulence and biofilm formation. Additionally, these molecules have the potential to be used in synthetic biology applications whereby these small molecules are used as inputs to switch on and off AI-2 receptors. In this review, we highlight the state-of-the-art in the development of small molecules that perturb AI-2 signaling in bacteria and offer our perspective on the future development and applications of these classes of molecules.

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“…aeruginosa has been one of the best model organisms in screening QQ organisms and compounds as they produce QS signal molecules (BHL and OdDHL) and the QS has a critical role in their biofilm formation (Hentzer et al, 2002;Smith and Iglewski, 2003b;Kim et al, 2008;Shetye et al, 2013). After verifying the decrease in QS induction by lowering AHL levels in the presence of the isolates, we examined if the QQ activity of the isolates could inhibit the biofilm formation by P. aeruginosa PAO1.…”

Section: Inhibition Of Biofilm Formation By Qq Isolatesmentioning

confidence: 99%

Characterization of Quorum-Quenching Bacteria Isolated from Biofouled Membrane Used in Reverse Osmosis Process

Moon¹,

Huang²,

Choi³

et al. 2014

The Korean Journal of Microbiology

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Acyl homoserine lactone (AHL) lactonase has been proved to be the AHL-degrading enzyme with the highest substrate specificity for AHL molecules and has shown a considerable potential as low-cost and efficient quorum quenching (QQ) technique. However, few studies focused on its inhibitory effect on biofilm formation which is also a quorum sensing (QS)-regulated phenomenon. In this study, QQ activity of six isolates from biofouled reverse osmosis membranes was studied using Chromobacterium violaceum CV026 and Agrobacterium tumefaciens NTL4 as biosensors under various conditions. All of the isolates belonged to the genus Bacillus and showed QQ activity regardless of the acyl chain length or substitution of AHL molecule. The isolates were capable of significantly inhibiting biofilm formation (46.7-58.3%) by Pseudomonas aeruginosa PAO1 and produced heat-sensitive extracellular QQ substances. The LC-MS analysis of the QQ activity of a selected isolate, RO1S-5, revealed the degradation of N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12 AHL) and the production of corresponding acyl homoserine (3-oxo-C12-HS), which indicated the activity of AHL lactonase. The broad AHL substrate range and high substrate specificity suggested that the isolate would be useful for the control of biofilm-related pathogenesis and biofouling in industrial processes. Keywords:Pseudomonas aeruginosa, AHL lactonase, biofilm, quorum quenching, quorum sensing *For correspondence. E-mail: ysoh@mju.ac.kr; Tel.: Quorum sensing (QS) allows bacteria to perceive the density of the surrounding bacterial populations using signal molecules such as N-acyl homoserine lactone (AHL) and to coordinately respond to this information by regulating expression of various genes (Smith and Iglewski, 2003a). QS has been known to play significant roles in many biological processes such as virulence factor production, bioluminescence, antibiotic production, sporulation, and biofilm formation (Novick and Geisinger, 2008;Ng and Bassler, 2009; Williams and Cámara, 2009).Among these, biofilm formation is one of the major contributors to the pathogenesis of many clinically important bacteria, and the loss of efficiency in medical, industrial, and environmental processes such as medically-implanted devices, oil drilling, paper production, food processing, fish farming, and membrane separation processes (Donlan, 2001; Defoirdt et al., 2004; Adonizio et al., 2008; Ammor et al., 2008; Wevers et al., 2009; Van Houdt and Michiels, 2010). Biofilm is not only ubiquitous and costly but also endows the cells with resistance to many antimicrobial agents, which has made it difficult to suggest prevalent techniques to control biofilm development (Davies et al., 1998;Stewart and Costerton, 2001;Hoffman et al., 2005). Therefore, there has been a high demand for effective strategies to control biofilm formation, and targeting the QS regulatory mechanism has been a highly considerable candidate due to the critical role of QS in biofilm formation (Davies et al., 1998;Smith and Iglewski,...

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Structures and biofilm inhibition activities of brominated furanones for Escherichia coli and Pseudomonas aeruginosa

Cited by 26 publications

References 38 publications

Incorporation of brominated furanone into Nafion polymer enhanced anti-biofilm efficacy

Incorporation of brominated furanone into Nafion polymer enhanced anti-biofilm efficacy

Small Molecule Inhibitors of AI-2 Signaling in Bacteria: State-of-the-Art and Future Perspectives for Anti-Quorum Sensing Agents

Characterization of Quorum-Quenching Bacteria Isolated from Biofouled Membrane Used in Reverse Osmosis Process

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