Structured surface wetting of a PTFE flow-cell for terahertz spectroscopy of proteins

Klokkou, Nicholas T.; Rowe, David J.; Bowden, Bethany M.; Sessions, Neil; West, Jonathan; Wilkinson, James S.; Apostolopoulos, Vasilis

doi:10.1016/j.snb.2021.131003

Cited by 12 publications

(5 citation statements)

References 44 publications

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“…Terahertz (THz) spectroscopy has shown great potential as a label-free, non-contact, and non-destructive technique for sensing chemical and biological substances [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Metamaterials operating in the THz range are particularly useful for detecting biological substances, including microorganisms and viruses [20][21][22][23][24][25][26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Developing a Novel Terahertz Fabry–Perot Microcavity Biosensor by Incorporating Porous Film for Yeast Sensing

Kim

Jun

Ahn

2023

Sensors

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We present a novel terahertz (THz) Fabry–Perot (FP) microcavity biosensor that uses a porous polytetrafluoroethylene (PTFE) supporting film to improve microorganism detection. The THz FP microcavity confines and enhances fields in the middle of the cavity, where the target microbial film is placed with the aid of a PTFE film having a dielectric constant close to unity in the THz range. The resonant frequency shift increased linearly with increasing amount of yeasts, without showing saturation behavior under our experimental conditions. These results agree well with finite-difference time-domain (FDTD) simulations. The sensor’s sensitivity was 11.7 GHz/μm, close to the optimal condition of 12.5 GHz/μm, when yeast was placed at the cavity’s center, but no frequency shift was observed when the yeast was coated on the mirror side. We derived an explicit relation for the frequency shift as a function of the index, amount, and location of the substances that is consistent with the electric field distribution across the cavity. We also produced THz transmission images of yeast-coated PTFE, mapping the frequency shift of the FP resonance and revealing the spatial distribution of yeast.

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Section: Introductionmentioning

confidence: 99%

Developing a Novel Terahertz Fabry–Perot Microcavity Biosensor by Incorporating Porous Film for Yeast Sensing

Kim

Jun

Ahn

2023

Sensors

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“…At present, there are mainly several detection methods in the field of aqueous sample detection, such as sample cell, 2 , 3 , 4 attenuated total reflection technology (ATR), and microfluidic chip. 5 , 6 Among them, ATR, which is widely used in infrared spectrum research, is mainly used to overcome the problems that are difficult to be solved by transmission detection method. Already there has been some research on detection of NaCl crystal, 7 milk, 8 glucose solution, 9 , 10 rat’s neuron, 11 human brain tumor tissues, 12 , 13 skin tissue, 14 and leaves.…”

Section: Introductionmentioning

confidence: 99%

Quantitative detection of THz-ATR spectra of aqueous samples under strong-field terahertz wave

Shi

Wang

et al. 2023

iScience

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“…These motions can be identified by fingerprint features in the THz transmission or absorption spectra, absorption changes, and phase changes (Zheng et al, 2014;Yan et al, 2016). Changes of biomolecules, such as change of formation, content, and state, strongly affect the THz spectral characteristics (Niehues et al, 2011;Bye et al, 2014;Klokkou et al, 2022). Recently, THz wave has been proved to have strong interactions with many biochemical molecules such as amino acids, proteins, and deoxyribonucleic acid (DNA) (Esser et al, 2018;Wu et al, 2020;Mancini et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Terahertz spectra of proteinuria and non-proteinuria

Xue

Mao²,

Peng³

et al. 2023

Front. Bioeng. Biotechnol.

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In clinical practice, proteinuria detection is of great significance in the diagnosis of kidney diseases. Dipstick analysis is used in most outpatient settings to semi-quantitatively measure the urine protein concentration. However, this method has limitations for protein detection, and alkaline urine or hematuria will cause false positive results. Recently, terahertz time-domain spectroscopy (THz-TDS) with strong hydrogen bonding sensitivity has been proven to be able to distinguish different types of biological solutions, which means that protein molecules in urine may have different THz spectral characteristics. In this study, we performed a preliminary clinical study investigating the terahertz spectra of 20 fresh urine samples (non-proteinuria and proteinuria). The results showed that the concentration of urine protein was positively correlated with the absorption of THz spectra at 0.5–1.2 THz. At 1.0 THz, the pH values (6, 7, 8, and 9) had no significant effect on the THz absorption spectra of urine proteins. The terahertz absorption of proteins with a high molecular weight (albumin) was greater than that of proteins with a low molecular weight (β2-microglobulin) at the same concentration. Overall, THz-TDS spectroscopy for the qualitative detection of proteinuria is not affected by pH and has the potential to discriminate between albumin and β2-microglobulin in urine.

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Structured surface wetting of a PTFE flow-cell for terahertz spectroscopy of proteins

Cited by 12 publications

References 44 publications

Developing a Novel Terahertz Fabry–Perot Microcavity Biosensor by Incorporating Porous Film for Yeast Sensing

Developing a Novel Terahertz Fabry–Perot Microcavity Biosensor by Incorporating Porous Film for Yeast Sensing

Quantitative detection of THz-ATR spectra of aqueous samples under strong-field terahertz wave

Terahertz spectra of proteinuria and non-proteinuria

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