Structured plant metabolomics for the simultaneous exploration of multiple factors

Vasil'ev, N. P.; Bernard, Julien; Lang, Gerhard K.; Grömping, Ulrike; Fischer, Rainer; Goepfert, Simon; Rudaz, Serge; Schillberg, Stefan

doi:10.1038/srep37390

Cited by 50 publications

(24 citation statements)

References 47 publications

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“…Further, the high chemodiversity of plant metabolites entails many different chemical properties. To find new candidates for priming, the application of multiple chromatography systems for untargeted metabolic profiling should be taken into account (e.g., Nakabayshi and Saito, 2015; Vasilev et al., 2016).…”

Section: Experimental Approachesmentioning

confidence: 99%

Induced, Imprinted, and Primed Responses to Changing Environments: Does Metabolism Store and Process Information?

Schwachtje¹,

Whitcomb²,

Firmino³

et al. 2019

Front. Plant Sci.

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Metabolism is the system layer that determines growth by the rate of matter uptake and conversion into biomass. The scaffold of enzymatic reaction rates drives the metabolic network in a given physico-chemical environment. In response to the diverse environmental stresses, plants have evolved the capability of integrating macro- and micro-environmental events to be prepared, i.e., to be primed for upcoming environmental challenges. The hierarchical view on stress signaling, where metabolites are seen as final downstream products, has recently been complemented by findings that metabolites themselves function as stress signals. We present a systematic concept of metabolic responses that are induced by environmental stresses and persist in the plant system. Such metabolic imprints may prime metabolic responses of plants for subsequent environmental stresses. We describe response types with examples of biotic and abiotic environmental stresses and suggest that plants use metabolic imprints, the metabolic changes that last beyond recovery from stress events, and priming, the imprints that function to prepare for upcoming stresses, to integrate diverse environmental stress histories. As a consequence, even genetically identical plants should be studied and understood as phenotypically plastic organisms that continuously adjust their metabolic state in response to their individually experienced local environment. To explore the occurrence and to unravel functions of metabolic imprints, we encourage researchers to extend stress studies by including detailed metabolic and stress response monitoring into extended recovery phases.

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Section: Experimental Approachesmentioning

confidence: 99%

Induced, Imprinted, and Primed Responses to Changing Environments: Does Metabolism Store and Process Information?

Schwachtje¹,

Whitcomb²,

Firmino³

et al. 2019

Front. Plant Sci.

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“…DoE is increasingly used and can be applied to several kinds of experiments, i.e., organic synthesis [ 64 ], analytical method development [ 65 ], and sample preparation [ 66 ]. Particularly, factorial designs represent an attractive approach for natural matrices extraction [ 48 ] and the systematic evaluation of plant secondary metabolism [ 67 ].…”

Section: Resultsmentioning

confidence: 99%

Microwave-Assisted Extraction and HPLC-UV-CD Determination of (S)-usnic Acid in Cladonia foliacea

et al. 2021

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During the years, many usnic acid (UA) conjugates have been synthesized to obtain potent endowed with biological properties. Since (S)-UA is less abundant in nature than (R)-enantiomer, it is difficult to source, thus precluding a deeper investigation. Among the lichens producing UA, Cladonia foliacea is a valuable (S)-UA source. In the present work, we report on a rapid HPLC-UV/PAD-CD protocol suitable for the analysis and the identification of the main secondary metabolites present in C. foliacea extract. Best results were achieved using XBridge Phenyl column and acetonitrile and water, which were both added with formic acid as mobile phase in gradient elution. By combining analytical, spectroscopical, and chiroptical analysis, the most abundant analyte was unambiguously identified as (S)-UA. Accordingly, a versatile microwave-assisted extractive (MAE) protocol, assisted by a design of experiment (DoE), to quantitatively recover (S)-UA was set up. The best result in terms of UA extraction yield was obtained using ethanol and heating at 80 °C under microwave irradiation for 5 min. Starting from 100 g of dried C. foliacea, 420 mg of (S)-UA were achieved. Thus, our extraction method resulted in a suitable protocol to produce (S)-UA from C. foliacea for biological and pharmaceutical investigation or commercial purposes.

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“…▶ Table 3 Selected examples of recombinant proteins expressed transiently in N. benthamiana. E16 mAb 339.9 µg/g FW [151] Zika virus c2A10G6 1.47 mg/g FW [131] IgG-ZE3 1.5 mg/g FW [152] ZIKV E 160 µg/g FW [153] FWfresh leaf weight; TPtotal protein olite or protein product levels in any heterologous expression system [107,108]. All these approaches may contribute to further optimization.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 99%

Medicinal Plants: Guests and Hosts in the Heterologous Expression of High-Value Products

Vasil'ev

2021

Planta Med

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Medicinal plants play an important dual role in the context of the heterologous expression of high-value pharmaceutical products. On the one hand, the classical biochemical and modern omics approaches allowed for the discovery of various genes encoding biosynthetic pathways in medicinal plants. Recombinant DNA technology enabled introducing these genes and regulatory elements into host organisms and enhancing the heterologous production of the corresponding secondary metabolites. On the other hand, the transient expression of foreign DNA in plants facilitated the production of numerous proteins of pharmaceutical importance. This review summarizes several success stories of the engineering of plant metabolic pathways in heterologous hosts. Likewise, a few examples of recombinant protein expression in plants for therapeutic purposes are also highlighted. Therefore, the importance of medicinal plants has grown immensely as sources for valuable products of low and high molecular weight. The next step ahead for bioengineering is to achieve more success stories of industrial-scale production of secondary plant metabolites in microbial systems and to fully exploit plant cell factoriesʼ commercial potential for recombinant proteins.

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Structured plant metabolomics for the simultaneous exploration of multiple factors

Cited by 50 publications

References 47 publications

Induced, Imprinted, and Primed Responses to Changing Environments: Does Metabolism Store and Process Information?

Induced, Imprinted, and Primed Responses to Changing Environments: Does Metabolism Store and Process Information?

Microwave-Assisted Extraction and HPLC-UV-CD Determination of (S)-usnic Acid in Cladonia foliacea

Medicinal Plants: Guests and Hosts in the Heterologous Expression of High-Value Products

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