Structured light scatteroscopy

Krishnaswamy, Venkataramanan; Elliott, Jonathan T.; McClatchy, David M.; Barth, Richard J.; Wells, Wendy A.; Pogue, Brian W.; Paulsen, Keith D.

doi:10.1117/1.jbo.19.7.070504

Cited by 25 publications

(26 citation statements)

References 11 publications

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“…Liquid phantoms of Intralipid (0.5 to 4% by volume) and bovine blood (0 to 240 μM) were measured at a spatial frequency of 0.5 mm -1 and wavelength of 730 nm showed unperceivable effects from the blood, as the signal was only sensitive to the Intralipid concentration 6 . Further, a bovine tissue sample was imaged with and without a thick layer of blood on its surface, which masked this tissue surface when viewed under white light but had essentially no effect on the high spatial frequency image 6 . This is particularly useful as surface blood was an identified as an artifact in diffuse imaging of breast tissue margins 3 .…”

Section: Resultsmentioning

confidence: 99%

High spatial frequency structured light imaging for intraoperative breast tumor margin assessment

McClatchy

Krishnaswamy

Kanick

et al. 2015

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII

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A new superficial wide-field imaging technique is presented, which utilizes high spatial frequency structured illumination to constrain the light sampling volume to a sub-diffuse regime. In this transport regime, the effects of absorption are drastically reduced and the sensitivity to local scattering from ultrastructrual alterations is increased. Absorption independence is validated with multiple experiments including a bovine blood-Intralipid solution matrix and avian tissue with superficial bovine blood. The resulting structured light demodulated images show a complete insensitivity to the blood over Hb concentrations of 0 -240 μM. Increased sensitivity to ultrastructual changes is demonstrated by imaging avian tissue with controlled morphological alterations including formalin-induced crosslinking. This imaging technique is currently being translated towards intraoperative assessment of breast tumor margins because of its ability to capture an entire lumpectomy margin in a single field of view, insensitivity to confounding surface blood present on lumpectomies, and its inherent scatter signal without the need of any model inversion. Further, a new lumpectomy marking system is introduced that allows for both the surgeon to mark the lumpectomy during excision and optical assessment of the specimen after the margins have been marked. Structured light images acquired intraoperatively of all margins of lumpectomy specimens are presented to show feasibility of clinical translation. Immediate future work will focus on developing a multi-spectral system.

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Section: Resultsmentioning

confidence: 99%

High spatial frequency structured light imaging for intraoperative breast tumor margin assessment

McClatchy

Krishnaswamy

Kanick

et al. 2015

Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIII

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“…Approaches that have quantitated sub-diffuse scattering parameters in biological tissue have classically been limited to the sampling of small volumes, usually sub-mm [2]. Recent work has shown that imaging with high spatial frequency (f x ) illumination patterns provides a method to sample a localized scatter signal in a wide field image acquisition [3]. This study advances the hypothesis that structured light imaging can be used to quantitatively extract sub-diffuse scattering parameters from macroscopic images of tissue.…”

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confidence: 87%

Macroscopic imaging of microscopic tissue scattering parameters using high spatial-frequency imaging

Kanick

McClatchy

Pogue

2015

Optics in the Life Sciences

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“…Recently, our group showed that macroscopic imaging based on structured light with a high-frequency photon density wave, whose periodicity was finer than the length scale of diffuse photon propagation, resulted in sub-diffusive signal localization in a wide-field geometry. A spectroscopic [25] and multi-frequency [26] interpretation of high spatial frequency reflectance provided sensitivity to, and quantified images of, sub-diffuse scattering parameters, including the reduced scattering coefficient,

μ_{s}^{'}

, and a phase function parameter,

γ = \frac{(1 - g_{2})}{(1 - g_{1})}

, that characterizes the backscatter likelihood of the medium and is expressed as a weighted ratio of the first two Legendre moments of the scattering phase function, g1 and g2 [3,27]. The current study applies sd-SFDI to validate wide-field quantitative images of the sub-diffusive scattering parameters in phantoms containing user-tuned phase functions and then applies the approach to image fresh ex vivo tissue samples.…”

Section: Introductionmentioning

confidence: 99%

Wide-field quantitative imaging of tissue microstructure using sub-diffuse spatial frequency domain imaging

McClatchy¹,

Rizzo²,

Wells³

et al. 2016

Optica

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Localized measurements of scattering in biological tissue provide sensitivity to microstructural morphology but have limited utility to wide-field applications, such as surgical guidance. This study introduces sub-diffusive spatial frequency domain imaging (sd-SFDI), which uses high spatial frequency illumination to achieve wide-field sampling of localized reflectances. Model-based inversion recovers macroscopic variations in the reduced scattering coefficient (μs′) and the phase function backscatter parameter (γ). Measurements in optical phantoms show quantitative imaging of user-tuned phase-function-based contrast with accurate decoupling of parameters that define both the density and the size-scale distribution of scatterers. Measurements of fresh ex vivo breast tissue samples revealed, for the first time, unique clustering of sub-diffusive scattering properties for different tissue types. The results support that sd-SFDI provides maps of microscopic structural biomarkers that cannot be obtained with diffuse wide-field imaging and characterizes spatial variations not resolved by point-based optical sampling.

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Structured light scatteroscopy

Cited by 25 publications

References 11 publications

High spatial frequency structured light imaging for intraoperative breast tumor margin assessment

High spatial frequency structured light imaging for intraoperative breast tumor margin assessment

Macroscopic imaging of microscopic tissue scattering parameters using high spatial-frequency imaging

Wide-field quantitative imaging of tissue microstructure using sub-diffuse spatial frequency domain imaging

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