Structured illumination microscopy and its new developments

Chen, Jianling; Qiu, Caimin; You, Minghai; Chen, Xiaogang; Yang, Hongqin; Xie, Shusen

doi:10.1142/s179354581630010x

Cited by 5 publications

(2 citation statements)

References 41 publications

(35 reference statements)

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“…Previous reports have shown that PI4P-containing vesicles are present at the site of mitochondrial division, but the role played by these vesicles in the highly coordinated steps of mitochondrial division remains unclear. As the morphology of mitochondria, microtubules, microfilaments, and the ER is heterogeneous, with ultrastructural features that are below the diffraction limit, we used SIM [ 22 , 23 , 24 ] to capture the dynamics of subcellular structure better. We found that PI4P-containing vesicles regulate mitochondrial division by changing the content of PI4P derived from Golgi apparatus which is consistent with recent studies.…”

Section: Discussionmentioning

confidence: 99%

PI4P-Containing Vesicles from Golgi Contribute to Mitochondrial Division by Coordinating with Polymerized Actin

Duan

Wei

Zhang

et al. 2023

IJMS

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Golgi-derived PI4P-containing vesicles play important roles in mitochondrial division, which is essential for maintaining cellular homeostasis. However, the mechanism of the PI4P-containing vesicle effect on mitochondrial division is unclear. Here, we found that actin appeared to polymerize at the contact site between PI4P-containing vesicles and mitochondria, causing mitochondrial division. Increasing the content of PI4P derived from the Golgi apparatus increased actin polymerization and reduced the length of the mitochondria, suggesting that actin polymerization through PI4P-containing vesicles is involved in PI4P vesicle-related mitochondrial division. Collectively, our results support a model in which PI4P-containing vesicles derived from the Golgi apparatus cooperate with actin filaments to participate in mitochondrial division by contributing to actin polymerization, which regulates mitochondrial dynamics. This study enriches the understanding of the pathways that regulate mitochondrial division and provides new insight into mitochondrial dynamics.

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Section: Discussionmentioning

confidence: 99%

PI4P-Containing Vesicles from Golgi Contribute to Mitochondrial Division by Coordinating with Polymerized Actin

Duan

Wei

Zhang

et al. 2023

IJMS

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“…Yang and his colleagues in Fuzhou, China presented two papers in this special issue. In the¯rst paper, 5 they introduced the principle and development of SIM, along with the combination of this technique with other°u orescence imaging techniques. In the second paper, 6 they analyzed the theory of typical polarized lights in the¯eld of focal spot imaging, and used simulated images of°uorescent beads to demonstrate the in°uence of polarized light of di®erent modes on the quality of images of SIM and STED (stimulated emission depletion) microscopy.…”

mentioning

confidence: 99%

Editorial — Introduction to the special issue on super-resolution optical microscopy

Huang

Zhu

2016

J. Innov. Opt. Health Sci.

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Super-resolution optical microscopy, sometimes called optical nanoscopy, refers to a new kind of far-¯eld optical microscopy which allows optical imaging with a resolution higher than the well-known resolution limit (or the Abbe di®raction limit) which stood for more than a century. The reported approaches that break the resolution limit involve either e®ectively reducing the focus region of the excitation light or stochastic separation and precise localization of single°uorophores. Super-resolution optical microscopy provides unprecedented opportunities for tackling outstanding fundamental questions in life science, medicine, materials, and many others that require non-invasive imaging with molecular level spatial resolution.In the past decade, a large number of studies have been made in the technology development and applications of super-resolution optical microscopy. Among them, researches from China appeared more than ten years ago, 1 and grew rapidly in the past several years. This special issue is intended to present some of the recent progresses from China. Original research papers and reviews included here cover a broad range of topics: development of new°uorescent probes and labeling strategies, selection and use of low-light cameras, new data analysis and reconstruction methods, new super-resolution imaging methods, and recent applications of super-resolution imaging in glycoscience.Xu and his colleagues 2 in Beijing, China summarized the photophysical and biochemical properties of the recently developed photomodulatable°uorescent proteins (PMFPs), and discussed the properties of PMFPs that are important for di®erent kinds of super-resolution imaging techniques. They also pointed out the need of brighter green PMFPs with enhanced contrast ratios and photostability for live-cell superresolution techniques, and red/far-red PMFPs with optimal photochemical characteristics for dual-color super-resolution imaging.The Wang group 3 in Changchun, China focused on the developments and challenges of super-resolution imaging in glycoscience, or more speci¯cally, the distribution and role of carbohydrates in cell membranes. They introduced the imaging principle and the available°uorescent probes for super-resolution imaging, and the strategies for labeling carbohydrates. They also summarized the applications of super-resolution imaging technology in spatial investigation of carbohydrates. This review is surely bene¯cial for researchers who are interested to use super-resolution imaging technology as a tool to obtain new insights into the study of glycoscience.To help readers understand the selection and optimal use of low-light cameras in super-resolution localization microscopy, one of the Guest Editors and his colleagues 4 provided a tutorial on how to fully access the performance of low-light cameras using a well-developed method called Photon Transfer Curve (PTC). They introduced brie°y the key parameters for characterizing low-light cameras, then explained how to use the PTC method for accessing the entire p...

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A Frequency Domain SIM Reconstruction Algorithm Using Reduced Number of Images

Lal

Shan

Zhao

et al. 2018

IEEE Trans. on Image Process.

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Conventional two-dimensional structured illumination microscopy (SIM) requires 9 raw images to reconstruct a super-resolved image. In order to increase the frame rate of 2DSIM, attempts are being made to reduce the number of raw SIM images. However, all the proposed SIM reconstruction algorithms (SIM-RA) capable of reconstructing super-resolution (SR) image with a reduced number of raw SIM images operate in the spatial domain. Here, we present a frequency domain SIM-RA based on ordinary least squares technique, which enables reconstruction of SR image using 4 raw SIM images. Unlike the spatial domain RA, which produces the SR image through iterative convergence, the presented RA provides a single step solution. It also reveals the fundamental limitation of least number of raw images required for resolution doubling in SIM.

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Structured illumination microscopy and its new developments

Cited by 5 publications

References 41 publications

PI4P-Containing Vesicles from Golgi Contribute to Mitochondrial Division by Coordinating with Polymerized Actin

PI4P-Containing Vesicles from Golgi Contribute to Mitochondrial Division by Coordinating with Polymerized Actin

Editorial — Introduction to the special issue on super-resolution optical microscopy

A Frequency Domain SIM Reconstruction Algorithm Using Reduced Number of Images

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