Structure-selective accumulation of polychlorinated biphenyls in Cucurbita pepo

Matsuo, Shinryo; Yamazaki, Kiyoshi; Gion, Keiko; Eun, Heesoo; Inui, Hideyuki

doi:10.1584/jpestics.g11-03

Cited by 23 publications

(16 citation statements)

References 22 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2B). The band intensities and the BCFs of PCDDs and PCDFs and phytoextraction of p,p9-DDE were also significantly positively correlated (White et al, 2003;Matsuo et al, 2011; r = 0.711 for PCDDs, r = 0.742 for PCDFs, and r = 0.780 for p,p9-DDE, P , 0.05 in all cases; Supplemental Fig. S2).…”

Section: Sds-page For Xylem Sapmentioning

confidence: 82%

“…Band intensities at 17 kD were significantly positively correlated with BCFs of PCBs (Matsuo et al, 2011; r = 0.669, P , 0.05; Fig. 2B).…”

Section: Sds-page For Xylem Sapmentioning

confidence: 86%

“…1B). The solubilization activities and BCFs of PCDDs and PCDFs have a trend to be positively correlated (Matsuo et al, 2011; r = 0.636, P = 0.0656 for PCDDs, r = 0.658, P = 0.0538 for PCDFs; data not shown). The BCFs used here were calculated by dividing the concentration of PCDDs, PCDFs, and PCBs in aerial parts including leaves and stems by the concentration in soil.…”

Section: Solubilization Activities Of Xylem Sap Toward Pyrenementioning

confidence: 88%

“…The expression levels of MLP-PG1 tended to decrease with an increase in a cultivar's ability to take up dioxins and dioxin-like compounds ( Fig. 5A; Matsuo et al, 2011). In contrast, the expression levels of MLP-GR3 in C. pepo ssp.…”

Section: Expression Analysis Of Mlp Genes In C Pepomentioning

confidence: 93%

“…The activity of boiled MG sap was similar to that of PG sap. The pyrene solubilization activities of xylem sap were significantly positively correlated with bioconcentration factors (BCFs) of PCBs (Matsuo et al, 2011;r = 0.781 [Pearson's correlation coefficient], P , 0.05; Fig. 1B).…”

Section: Solubilization Activities Of Xylem Sap Toward Pyrenementioning

confidence: 99%

See 4 more Smart Citations

A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants

et al. 2013

Self Cite

View full text Add to dashboard Cite

This is the first report, to our knowledge, to reveal important factors by which members of the Cucurbitaceae family, such as cucumber (Cucumis sativus), watermelon (Citrullus lanatus), melon (Cucumis melo), pumpkin (Cucurbita pepo), squash (C. pepo), and zucchini (C. pepo), are selectively polluted with highly toxic hydrophobic contaminants, including organochlorine insecticides and dioxins. Xylem sap of C. pepo ssp. pepo, which is a high accumulator of hydrophobic compounds, solubilized the hydrophobic compound pyrene into the aqueous phase via some protein(s). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of xylem sap of two C. pepo subspecies revealed that the amount of 17-kD proteins in C. pepo ssp. pepo was larger than that in C. pepo ssp. ovifera, a low accumulator, suggesting that these proteins may be related to the translocation of hydrophobic compounds. The protein bands at 17 kD contained major latex-like proteins (MLPs), and the corresponding genes MLP-PG1, MLP-GR1, and MLP-GR3 were cloned from the C. pepo cultivars Patty Green and Gold Rush. Expression of the MLP-GR3 gene in C. pepo cultivars was positively correlated with the band intensity of 17-kD proteins and bioconcentration factors toward dioxins and dioxin-like compounds. Recombinant MLP-GR3 bound polychlorinated biphenyls immobilized on magnetic beads, whereas recombinant MLP-PG1 and MLP-GR1 did not. These results indicate that the high expression of MLP-GR3 in C. pepo ssp. pepo plants and the existence of MLP-GR3 in their xylem sap are related to the efficient translocation of hydrophobic contaminants. These findings should be useful for decreasing the contamination of fruit of the Cucurbitaceae family as well as the phytoremediation of hydrophobic contaminants.

show abstract

Section: Sds-page For Xylem Sapmentioning

confidence: 82%

“…Band intensities at 17 kD were significantly positively correlated with BCFs of PCBs (Matsuo et al, 2011; r = 0.669, P , 0.05; Fig. 2B).…”

Section: Sds-page For Xylem Sapmentioning

confidence: 86%

Section: Solubilization Activities Of Xylem Sap Toward Pyrenementioning

confidence: 88%

Section: Expression Analysis Of Mlp Genes In C Pepomentioning

confidence: 93%

Section: Solubilization Activities Of Xylem Sap Toward Pyrenementioning

confidence: 99%

See 3 more Smart Citations

A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

How does the Cucurbitaceae family take up organic pollutants (POPs, PAHs, and PPCPs)?

Fujita

Inui

2021

Rev Environ Sci Biotechnol

View full text Add to dashboard Cite

Distribution of PCDD/Fs and dioxin-like PCBs in sediment and plants from a contaminated salt marsh (Tejo estuary, Portugal)

Nunes

Vernisseau

Marchand

et al. 2013

Environ Sci Pollut Res

View full text Add to dashboard Cite

Concentrations and profiles of 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (dl-PCBs) were investigated in sediment and plants collected from a salt marsh in the Tejo estuary, Portugal. The highest PCDD/F and dl-PCB concentrations were detected in uncolonized sediments, averaging 325.25 ± 57.55 pg g(-1) dry weight (dw) and 8,146.33 ± 2,142.14 pg g(-1) dw, respectively. The plants Sarcocornia perennis and Halimione portulacoides growing in PCDD/F and dl-PCB contaminated sediments accumulated contaminants in roots, stems, and leaves. It was observed that PCDD/F and dl-PCB concentrations in roots were significantly lower in comparison with stems and leaves. In general, concentration of ΣPCDD/Fs and Σdl-PCBs in H. portulacoides tissues were found to be twofold higher than those in S. perennis, indicating a difference in the accumulation capability of both species. Furthermore, congener profiles changed between sediments and plant tissues, reflecting a selective accumulation of low chlorinated PCDD/Fs and non-ortho dl-PCBs in plants.

show abstract

Structure-selective accumulation of polychlorinated biphenyls in Cucurbita pepo

Cited by 23 publications

References 22 publications

A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants

A Major Latex-Like Protein Is a Key Factor in Crop Contamination by Persistent Organic Pollutants

How does the Cucurbitaceae family take up organic pollutants (POPs, PAHs, and PPCPs)?

Distribution of PCDD/Fs and dioxin-like PCBs in sediment and plants from a contaminated salt marsh (Tejo estuary, Portugal)

Contact Info

Product

Resources

About