Structure Relationship of Cationic Lipids on Gene Transfection Mediated by Cationic Liposomes

Paecharoenchai, Orapan; Niyomtham, Nattisa; Apirakaramwong, Auayporn; Ngawhirunpat, Tanasait; Rojanarata, Theerasak; Yingyongnarongkul, Boon‐ek; Opanasopit, Praneet

doi:10.1208/s12249-012-9857-5

Cited by 27 publications

(24 citation statements)

References 25 publications

(25 reference statements)

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“…However, a decrease in transfection efficiency was observed if the weight ratio was increased above that yielding the highest transfection efficiency. This finding is in agreement with our previous reports (24,25). The highest transfection efficiency observed for the niosomes-C14, niosome-C16, and niosomes-C18 was at weight ratios of 20, 30, and 10, respectively.…”

Section: Characterization Of Cationic Niosomes and Niosomes/dna Complsupporting

confidence: 93%

Nonionic Surfactant Vesicles Composed of Novel Spermine-Derivative Cationic Lipids as an Effective Gene Carrier In Vitro

et al. 2014

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In the present study, nonionic surfactant vesicles (niosomes) formulated with Span 20, cholesterol, and novel synthesized spermine-based cationic lipids with four hydrocarbon tails in a molar ratio of 2.5:2.5:1 were investigated as a gene carrier. The effects of the structure of the cationic lipids, such as differences in the acyl chain length (C14, C16, and C18) of the hydrophobic tails, as well as the weight ratio of niosomes to DNA on transfection efficiency and cell viability were evaluated in a human cervical carcinoma cell line (HeLa cells) using pDNA encoding green fluorescent protein (pEGFP-C2). The niosomes were characterized both in terms of morphology and of size and charge measurement. The formation of complexes between niosomes and DNA was verified with a gel retardation assay. The transfection efficiency of these cationic niosomes was in the following order: spermine-C18 > spermine-C16 > spermine-C14. The highest transfection efficiency was obtained for transfection with spermine-C18 niosomes at a weight ratio of 10. Additionally, no serum effect on transfection efficiency was observed. The results from a cytotoxicity and hemolytic study showed that the cationic niosomes were safe in vitro. In addition, the cationic niosomes showed good physical stability for at least 1 month at 4°C. Therefore, the cationic niosomes offer an excellent prospect as an alternative gene carrier.

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Section: Characterization Of Cationic Niosomes and Niosomes/dna Complsupporting

confidence: 93%

Nonionic Surfactant Vesicles Composed of Novel Spermine-Derivative Cationic Lipids as an Effective Gene Carrier In Vitro

et al. 2014

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“…21,[24][25][26][27] On the other hand, we have previously shown that the use of cationic liposomes composed of EPOPC and Chol, at a 1:1 molar ratio, results in considerably higher biological activities than those obtained with other lipid-based formulations. [13][14][15][16][17]28 In this regard, the main goal of this work consisted of the development of a new gene delivery lipid-based nanosystem for HCC, targeting the ASGP-R, based on cationic liposomes composed of EPOPC, Chol and lactosyl-PE.…”

Section: Discussionmentioning

confidence: 99%

“…[9][10][11] Among these, cationic liposome/DNA complexes (lipoplexes) have been the most extensively studied, since they present higher gene delivery efficiency, both in vitro and in vivo, than that observed with other non-viral gene delivery systems. 12,13,14 Our studies have demonstrated that cationic liposomes composed of 1-palmitoyl-2-oleoyl-snglycero-3-ethylphosphocholine (EPOPC) and cholesterol (Chol), at a molar ratio of 1:1, have the ability to mediate efficient gene delivery in different tissues and cells lines. 13,15 Lipoplexes prepared with these cationic liposomes were shown to exhibit higher levels of transfection than those obtained with nanosystems prepared with other cationic liposome formulations.…”

Section: Introductionmentioning

confidence: 99%

Increased gene delivery efficiency and specificity of a lipid-based nanosystem incorporating a glycolipid

Magalhães¹,

Farinha²,

Lima³

et al. 2014

IJN

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Hepatocellular carcinoma (HCC) is the third most common cause of death related to cancer diseases worldwide. The current treatment options have many limitations and reduced success rates. In this regard, advances in gene therapy have shown promising results in novel therapeutic strategies. However, the success of gene therapy depends on the efficient and specific delivery of genetic material into target cells. In this regard, the main goal of this work was to develop a new lipid-based nanosystem formulation containing the lipid lactosyl-PE for specific and efficient gene delivery into HCC cells. The obtained results showed that incorporation of 15% of lactosyl-PE into liposomes induces a strong potentiation of lipoplex biological activity in HepG2 cells, not only in terms of transgene expression levels but also in terms of percentage of transfected cells. In the presence of galactose, which competes with lactosyl-PE for the binding to the asialoglycoprotein receptor (ASGP-R), a significant reduction in biological activity was observed, showing that the potentiation of transfection induced by the presence of lactosyl-PE could be due to its specific interaction with ASGP-R, which is overexpressed in HCC. In addition, it was found that the incorporation of lactosyl-PE in the nanosystems promotes an increase in their cell binding and uptake. Regarding the physicochemical properties of lipoplexes, the presence of lactosyl-PE resulted in a significant increase in DNA protection and in a substantial decrease in their mean diameter and zeta potential, conferring them suitable characteristics for in vivo application. Overall, the results obtained in this study suggest that the potentiation of the biological activity induced by the presence of lactosyl-PE is due to its specific binding to the ASGP-R, showing that this novel formulation could constitute a new gene delivery nanosystem for application in therapeutic strategies in HCC.

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“…18) Recently, we described the cationic lipids 1 19) and 2 20,21) (Fig. 1) which exhibited transfection efficiency higher than and comparable to the commercially available transfection reagents.…”

Section: )mentioning

confidence: 95%

Synergistic Effect of Cationic Lipids with Different Polarheads, Central Core Structures and Hydrophobic Tails on Gene Transfection Efficiency

Niyomtham

Apiratikul

Chanchang

et al. 2014

Biological & Pharmaceutical Bulletin

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Lipid-mediated delivery of DNA into cells holds great promise both for gene therapy and basic research applications. The primary approach to improving transfection efficiency is the design and synthesis of novel cationic lipids. Alternatively, using the synergistic effect of different cationic mixtures can provide another approach to increasing transfection efficiency. This paper describes the synergistic effect of lipids with different polarheads, central core structures and hydrophobic tails. The enhancement of cellular transfection into HEK293 cells was observed by combining two lipids having aminoglycerol and di(hydroxylethyl)amino core structures at a 1 : 1 weight ratio. Additionally, the liposome formation of these lipids with the helper lipid, 1,2-dioleoyl-propyl-3-phosphatidylethanolamine (DOPE), at the weight ratio of 1 : 1 can provide higher transfection efficiency into HEK293, MCF-7 and HeLa cells than Lipofectamine™ 2000. Our finding indicated that cationic liposomes comprised of a mixture of lipids with different polarheads, central core structures and hydrophobic tails should be very promising in liposome-mediated gene delivery in vitro and in vivo.Key words cationic liposome; synergistic effect; DNA delivery; non-viral vector.Gene therapy has gained significant attention over past two decades as an alternative method to treat genetic disorders 1,2) as well as cancers.3) One fundamental of gene therapy is the delivery system that can introduce and stabilize genetic material. Since free oligonucleotides and DNA are rapidly degraded by serum nucleases, 4) many efforts are focused on developing the carriers to protect and deliver these materials into targeted cells. Two delivery systems namely viral and non-viral vectors are currently developed to solve the problems. Viral carriers have known to be one of the most effective gene delivery methods for in vivo application. [5][6][7][8] Over one thousand gene therapy clinical trials have been completed or approved; two-thirds of which performed by viral vectors. 8)However, the limitation of viral vectors concerning toxicity, immunogenicity, scale-up procedures and their relatively small capacity for therapeutic DNA has prompted the development non-viral vectors. Delivery systems based on non-viral vectors, for example cationic lipids, dendrimers or cationic polymers, have been the focus of much recent research.9) Non-viral systems have proved to be generally less toxic or immunogenic, more easily to produce and a greater stability.Cationic liposomes are one of the most extensively studied of non-viral vectors because of their lesser immunogenic nature, ease of production and handle, and ability to deliver large pieces of DNA. Cationic liposomes, like other non-viral vectors bearing positive charge, interact with the negatively charged phosphate backbone of nucleic acids to form a compact structure and facilitate cellular uptake by endocytic routes. [10][11][12][13] Since the first application of cationic lipid in DNA delivery, 14) numerous cationic lipids...

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Structure Relationship of Cationic Lipids on Gene Transfection Mediated by Cationic Liposomes

Cited by 27 publications

References 25 publications

Nonionic Surfactant Vesicles Composed of Novel Spermine-Derivative Cationic Lipids as an Effective Gene Carrier In Vitro

Nonionic Surfactant Vesicles Composed of Novel Spermine-Derivative Cationic Lipids as an Effective Gene Carrier In Vitro

Increased gene delivery efficiency and specificity of a lipid-based nanosystem incorporating a glycolipid

Synergistic Effect of Cationic Lipids with Different Polarheads, Central Core Structures and Hydrophobic Tails on Gene Transfection Efficiency

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