Structure–reactivity relations for DC-magnetron sputtered Cu-layers during E. coli inactivation in the dark and under light

Castro, Camilo A.; Sanjinés, R.; Pulgarín, C.; Osorio, Paula; Giraldo, Sonia A.; Kiwi, J.

doi:10.1016/j.jphotochem.2010.06.030

Cited by 43 publications

(31 citation statements)

References 18 publications

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“…Thus, coatings presenting an improved stability and a suitable amount of Cu may represent an improvement to the present state of the art (O'Gorman and Humphreys 2012). Recently developed uniform, adhesive Cu-nanoparticle films obtained by direct current magnetron sputtering (DCMS) appear to satisfy these requirements (Castro et al 2010). Moreover, we have recently shown that Cu nanoparticles sputtered on flexible surfaces possess rapid bactericidal activity against a number of MRSA isolates (Rio et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Bactericidal activity and mechanism of action of copper-sputtered flexible surfaces against multidrug-resistant pathogens

Ballo

Rtimi

Mancini

et al. 2016

Appl Microbiol Biotechnol

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Using direct current magnetron sputtering (DCMS), we generated flexible copper polyester surfaces (Cu-PES) and investigated their antimicrobial activity against a range of multidrug-resistant (MDR) pathogens including eight Gram-positive isolates (three methicillin-resistant Staphylococcus aureus [MRSA], four vancomycin-resistant enterococci, one methicillin-resistant Staphylococcus epidermidis) and four Gram-negative strains (one extended-spectrum β-lactamase-producing [ESBL] Escherichia coli, one ESBL Klebsiella pneumoniae, one imipenem-resistant Pseudomonas aeruginosa, and one ciprofloxacin-resistant Acinetobacter baumannii). Bactericidal activity (≥3 log10 CFU reduction of the starting inoculum) was reached within 15-30 min exposure to Cu-PES. Antimicrobial activity of Cu-PES persisted in the absence of oxygen and against both Gram-positive and Gram-negative bacteria containing elevated levels of catalases, indicating that reactive oxygen species (ROS) do not play a primary role in the killing process. The decrease in cell viability of MRSA ATCC 43300 and Enterococcus faecalis V583 correlated with the progressive loss of cytoplasmic membrane integrity both under aerobic and anaerobic conditions, suggesting that Cu-PES mediated killing is primarily induced by disruption of the cytoplasmic membrane function. Overall, we here present novel antimicrobial copper surfaces with improved stability and sustainability and provide further insights into their mechanism of killing.

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Section: Introductionmentioning

confidence: 99%

Bactericidal activity and mechanism of action of copper-sputtered flexible surfaces against multidrug-resistant pathogens

Ballo

Rtimi

Mancini

et al. 2016

Appl Microbiol Biotechnol

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“…Figure 7(a), trace 4, shows that the redox potential Cu-nanoparticles induce bacterial inactivation within reasonable times. 7,10,16 Finally, Fig. 7(a), trace 5, shows no bacterial inactivation on the polyester surface under actinic light.…”

Section: -4 Rtimimentioning

confidence: 87%

“…The vacuum in the chamber was of 0.1 Pa, and the distance between the Cu-target and the polyester was $10 cm. [16][17][18] The nominal film thickness was determined with a profilometer (Alphastep500, TENCOR) on silica wafers. Figure 1 shows C and the thickness of the polyester was 6130 lm 610%.…”

Section: Methodsmentioning

confidence: 99%

“…Cu-surfaces reported by our laboratory for Cu sputtered on cotton showing significant antibacterial activity. 16 The present study addresses: (1) the optimization of Cu deposition by DC-magnetron sputtering on polyester as a follow up of textiles showing bactericide properties, 17 (2) the investigation of the Cu-layers on polyester inducing E. coli inactivation in the dark as recently shown by Cu-textiles inactivating Methicillin-resistant Staphylococcus aureus, 18 (3) the effect of actinic light on Cu/CuO-polyester when inactivating E. coli, (4) the stereomicroscopy in the dark and under light of during E. coli inactivation, (5) the XPS deconvolution of the Cu-oxidation states intervening in bacterial inactivation, and finally, (6) the Cu released during bacterial inactivation during the inactivation as monitored by inductively coupled plasma-mass spectroscopy (ICP-MS). Haenle et al, 19 have shown that Cu possess the most favorable ratio of bacterial activity/toxicity at a cytotoxic biocompatible level.…”

Section: Introductionmentioning

confidence: 99%

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Accelerated Escherichia coli inactivation in the dark on uniform copper flexible surfaces

et al. 2014

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The bacterial inactivation of Escherichia coli on Cu/CuO-polyester surfaces prepared by direct current magnetron sputtering was investigated in the dark and under actinic light (360 nm≤ λ ≤ 720 nm; 4.1 mW/cm2) as used commonly in hospital facilities. In the dark, complete bacterial inactivation (6log10 reduction) was observed within 150 min and under actinic light within 45 min. Sputtered samples led to nanoparticulate uniform Cu/CuO films ∼70 nm thick. The deposition rate used was 2.2×1015 atoms/cm2 s as determined by profilometry. X-ray fluorescence was used to determine the sample Cu-content and transmission electron microscopy determined Cu-particles ∼20 ± 5 nm in size. The film optical absorption was observed to increase with Cu-content of the sample by diffuse reflection spectroscopy. The bacterial inactivation involved redox processes between Cu/CuO-polyester and the bacteria as observed by x-ray photoelectron spectroscopy. During sample recycling, the amount of Cu-release was determined by inductively coupled plasma-mass spectroscopy. The values required for E. coli inactivation were below the cytotoxicity level threshold allowed for mammalian cells. The E. coli inactivation by Cu/CuO-polyester seems to involve an oligodynamic effect since bacterial inactivation was achieved at very low Cu-concentrations.

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“…DCP was used to sputter Ag and was operated at 50 kHz with 15% reversed voltage. The sputtering current was fixed at 280 mA (14.3 mA/cm 2 ) providing a negative voltage of −500 V, 75 V as reverse voltage (15% of 500 V) and a power of 140 W. Details regarding sputtering Ag on different textiles using the unit available in our laboratory have been described recently [6,10,11,23,24].…”

Section: Sputtering Of Tin Underlayers and Tin-ag Films Thickness Camentioning

confidence: 99%

Photocatalysis/catalysis by innovative TiN and TiN-Ag surfaces inactivate bacteria under visible light

Rtimi

Baghriche

Sanjinés

et al. 2012

Applied Catalysis B: Environmental

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a b s t r a c tThis study presents the design, preparation, testing and characterization of TiN and TiN-Ag nanoparticulate films leading to photocatalytic and catalytic inactivation of Escherichia coli. When Ti was sputtered in N 2 atmosphere, the TiN films unexpectedly revealed semiconductor properties when irradiated under visible light due to the formation of TiO 2 showing absorption in the visible spectral region. In TiN-Ag films, Ag enhances the photocatalytic activity of TiN leading to faster bacterial inactivation. Evidence for the presence of TiO 2 and TiN in the films is presented by XPS. The TiN layers 50 nm thick sputtered by DC for 3 min led to complete inactivation of E. coli within 120 min. But TiN layers with a thickness >50 nm hinder the surface diffusion of charges reducing bacterial inactivation. The rate of TiN deposition was ∼1.4 × 10 15 atoms TiN/cm 2 s. For the TiN-polyester samples under visible light a 3 log 10 bacterial reduction (99.9%) was observed within 30 min while for TiN-Ag samples the same bacterial reduction was attained within ∼15 min. The absorption of the TiN-Ag samples in Kubelka-Munk (KM) units was directly proportional to the E. coli inactivation kinetics. TiN-Ag plasmon nanostructures are concurrently formed under low intensity visible light and accelerated bacterial inactivation. This study shows that TiN films have the potential to replace Ag-based disinfection materials leaching Ag into the environment.

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Structure–reactivity relations for DC-magnetron sputtered Cu-layers during E. coli inactivation in the dark and under light

Cited by 43 publications

References 18 publications

Bactericidal activity and mechanism of action of copper-sputtered flexible surfaces against multidrug-resistant pathogens

Bactericidal activity and mechanism of action of copper-sputtered flexible surfaces against multidrug-resistant pathogens

Accelerated Escherichia coli inactivation in the dark on uniform copper flexible surfaces

Photocatalysis/catalysis by innovative TiN and TiN-Ag surfaces inactivate bacteria under visible light

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