Structure, orientation, and segregation of the sex trivalent in <i>Pyrophorus luminosus</i> III. (Coleoptera, Elateridae)

Virkki, Niilo; Flores, Marot Nelmida; Escudero, Joaquina de la Torre

doi:10.1139/g84-050

Cited by 16 publications

(26 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The additional C-bands in these beetles and in D. mimas and I. inhiata probably arose by small tandem duplications, as proposed by King and John (1980). Data on localization of NORs in Coleoptera suggest that an autosome pair functioning as nucleolus organiser appears to be widely distributed in this order (Virkki, 1983;Virkki et al, 1984;Postiglioni and Brum-Zorrilla, 1988;Colomba et al, 2000a;Moura et al, 2003). This stands in contrast to most Scarabaeidae, where the NOR is found in the sex bivalent (Bione, 1999;Moura, 2002) NOR activity at the beginning of the meiotic prophase is widely observed in a large number of organisms, including Coleoptera species.…”

Section: Discussionmentioning

confidence: 87%

A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques

2005

View full text Add to dashboard Cite

Meiotic and mitotic chromosomes of Isocopris inhiata and Diabroctis mimas were studied by standard staining procedures, C-banding, silver nitrate staining and FISH using Apis mellifera 28S rDNA as probe. Isocopris inhiata presented a 2n = 18 (8II+ Xy p ) karyotype, composed of meta-submetacentric chromosomes with gradual reduction in size. The karyotype of D. mimas was 2n = 20 (9II+ Xy p ), composed of meta-submetacentric (pairs 1, 2, 3, 4 and 7) and acrocentric (pairs 5, 6, 8 and 9) chromosomes, with gradual reduction in size. Analysis of constitutive heterochromatin revealed similar C-banding patterns for both species, showing pericentromeric and telomeric bands and diphasic chromosomes. In addition, the X chromosomes of these species were found to be almost completely heterochromatic. The presence of chromocenters was checked in one or more phases of prophase I of these species. All heterochromatin reacted positively for the silver stain. By FISH analysis we were able to locate the rDNA in medium-size autosome pairs in both species and in the X chromosome of D. mimas.

show abstract

Section: Discussionmentioning

confidence: 87%

A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques

2005

View full text Add to dashboard Cite

show abstract

“…For example, in Gymnopleurus sturni (Vitturi et al 1999) and Thorectes intermedius (Colomba et al 2000) GC-rich sequences were detected in all the chromosomes while Lyogenys fuscus presented AT-rich sequences in every karyotype complement studied (Moura et al, 2003). Data regarding the location of NORs in Coleoptera have suggested that a pair of nucleolar organizer autosomes is widely distributed in this order (Virkki, 1983;Virkki et al, 1984). In representatives of the family Scarabaeidae rDNA sites are generally found on the X chromosome, although sites located on autosomes have been reported for Phyllophaga (Phyllophaga) aff capillata and Gymnopleurus sturni (Moura et al, 2003;Colomba et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Karyotype, C-and fluorescence banding pattern, NOR location and FISH study of five Scarabaeidae (Coleoptera) species

et al. 2005

View full text Add to dashboard Cite

Meiotic chromosomes obtained from members of the coleopteran subfamilies Rutelinae and Dynastinae were studied using standard and silver nitrate staining, C-banding, base-specific fluorochromes and fluorescent in situ hybridization (FISH). The study presents detailed karyotipic descripitions of three Rutelinae species (Geniates borelli, Macraspis festiva and Pelidnota pallidipennis), and two Dynastinae species (Lygirus ebenus and Strategus surinamensis hirtus) with special emphasis on the distribution and variability of constitutive heterochromatin and the nucleolar organizer region (NOR). We found that for G. borelli, P. pallidipennis, L. ebenus and S. s hirtus the karyotype was 2n = 20 (9II + Xy p ), with G. borelli, P. pallidipennis and L. ebenus showed meta-submetacentric chromosomes which gradually decreased in size. For Macraspis festiva the karyotype was 2n = 18 (8II + Xy p ). In L. ebenus we found that the NOR was located on an autosome, but in the other four species it occurred on the sex bivalents. In all five species the constitutive heterochromatin (CH) was predominantly pericentromeric while the X chromosomes were almost completely heterochomatic, although CMA 3 /DA/DAPI staining showed intra and interspecific variation in the bright fluorescence of the constitutive heterochromatin. The FISH technique showed rDNA sites on the X chromosome of the Rutelinae species.

show abstract

“…Among the 17 species for which the number of NORs is determined, 14 have one site located on one autosomal pair, two species have two pairs of autosomes with NORs, and one species has only one NOR located on the neoX chromosome (Petitpierre, 1970(Petitpierre, , 1976(Petitpierre, , 1996Virkki, 1983;Virkki & Denton, 1987;Postiglioni et al, 1990Postiglioni et al, , 1991Yadav et al, 1992;Schneider et al, 2002;Gómez-Zurita et al, 2004;Almeida et al, 2006Almeida et al, , 2010.…”

Section: Methodsmentioning

confidence: 99%

Chromosome mapping of 28S ribosomal genes in 11 species of Cassidinae (Coleoptera: Chrysomelidae)

Lopes¹,

Fernandes²,

Schneider³

2017

Eur. J. Entomol.

View full text Add to dashboard Cite

Structure, orientation, and segregation of the sex trivalent in Pyrophorus luminosus III. (Coleoptera, Elateridae)

Cited by 16 publications

References 1 publication

A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques

A study of the constitutive heterochromatin and nucleolus organizer regions of Isocopris inhiata and Diabroctis mimas (Coleoptera: Scarabaeidae, Scarabaeinae) using C-banding, AgNO3 staining and FISH techniques

Karyotype, C-and fluorescence banding pattern, NOR location and FISH study of five Scarabaeidae (Coleoptera) species

Chromosome mapping of 28S ribosomal genes in 11 species of Cassidinae (Coleoptera: Chrysomelidae)

Contact Info

Product

Resources

About