Structure of water at zwitterionic copolymer film–liquid water interfaces as examined by the sum frequency generation method

Kondo, Takuya; Nomura, Kouji; Gemmei‐Ide, Makoto; Kitano, Hiromi; Noguchi, Hidenori; Uosaki, Kohei; Saruwatari, Yoshiyuki

doi:10.1016/j.colsurfb.2013.08.051

Cited by 43 publications

(35 citation statements)

References 41 publications

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“…Previously, Raman and infrared spectroscopy studies have indicated that the hydrogen‐bonded network structure of water in the vicinity of zwitterionic and amphoteric polymers is not disturbed; in contrast, ordinary polyelectrolytes cause significant change to the structure of vicinal water. A similar tendency was observed for zwitterionic copolymer films, zwitterionic and amphoteric (charge‐neutralized) polymer brushes, and self‐assembled monolayers using the sum frequency generation method. Taking into account the resistance of zwitterionic and amphoteric copolymer films and polymer brushes to the non‐specific adsorption of proteins and cell adhesion, it is possible that one of the most important factors responsible for the biocompatibility of these materials is that they do not significantly alter the local structure of water.…”

Section: Discussionsupporting

confidence: 70%

Optimization of the composition of zwitterionic copolymers for the easy-construction of bio-inactive surfaces

Nishida

Nakaji-Hirabayashi

Kitano

et al. 2016

J. Biomed. Mater. Res.

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Random copolymers (S-PCMBx) of the zwitterionic monomer carboxymethylbetaine (CMB) and a small percentage of 3-methacryloyloxypropyl trimethoxysilane with various composition ratios were synthesized in ethanol using 2,2'-azobisisobutyronitrile as the initiator. An S-PCMBx layer formed on the glass substrate after soaking in the copolymer solution and had a thickness of 2-3 nm. The S-PCMBx-modified surface was highly hydrophilic and suppressed both the non-specific adsorption of protein (bovine serum albumin) and NIH3T3-fibroblast adhesion. The degree of adsorption suppression increased with increasing copolymer CMB content with a maximum at 90 mol % CMB. In contrast, the modification of the glass substrate with a PCMB homopolymer terminally modified with a trimethoxysilyl group did not effectively suppress protein adsorption and cell adhesion due to the low graft density. The importance of balancing the number of fixation points and the length of the zwitterionic polymer loops to produce bio-inactive metal oxide surfaces is suggested. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2029-2036, 2016.

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Section: Discussionsupporting

confidence: 70%

Optimization of the composition of zwitterionic copolymers for the easy-construction of bio-inactive surfaces

Nishida

Nakaji-Hirabayashi

Kitano

et al. 2016

J. Biomed. Mater. Res.

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“…Conventional synthetic biocompatible polymers such as PMPC, poly(sulfobetaine methacrylate), poly(carboxybetaine methacrylate), and poly(ethylene glycol) (PEG) are known to demonstrate low protein adsorption and/or no platelet adhesion [35–39]. PDL cells hardly adhered and proliferated on PMPC, which was consistent with the findings of a previous study by Iwasaki et al, who reported that adhesion of human promyelocytic leukemia cells and human uterine cervical cancer cells was completely suppressed on PMPC [36].…”

Section: Resultsmentioning

confidence: 99%

Adhesion and Proliferation of Human Periodontal Ligament Cells on Poly(2-methoxyethyl acrylate)

Kitakami

Aoki

Sato

et al. 2014

BioMed Research International

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Human periodontal ligament (PDL) cells obtained from extracted teeth are a potential cell source for tissue engineering. We previously reported that poly(2-methoxyethyl acrylate) (PMEA) is highly biocompatible with human blood cells. In this study, we investigated the adhesion, morphology, and proliferation of PDL cells on PMEA and other types of polymers to design an appropriate scaffold for tissue engineering. PDL cells adhered and proliferated on all investigated polymer surfaces except for poly(2-hydroxyethyl methacrylate) and poly[(2-methacryloyloxyethyl phosphorylcholine)-co-(n-butyl methacrylate)]. The initial adhesion of the PDL cells on PMEA was comparable with that on polyethylene terephthalate (PET). In addition, the PDL cells on PMEA spread well and exhibited proliferation behavior similar to that observed on PET. In contrast, platelets hardly adhered to PMEA. PMEA is therefore expected to be an excellent scaffold for tissue engineering and for culturing tissue-derived cells in a blood-rich environment.

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“…The correlation between inert properties of polymer materials to vicinal water and their biocompatibility has previously been assessed by considering the structure of water in the vicinity of charge-neutralized and zwitterionic polymers, which was investigated using Raman, infrared, and sum frequency generation spectroscopies [39][40][41][42][43][44]. These studies suggest that the surface of amphoteric copolymers containing comparable content of MA and DMAEMA drastically suppresses both the orientation of vicinal water molecules, and the adsorption/adhesion of biomolecules such as proteins and cells [39].…”

Section: Cell Adhesionmentioning

confidence: 99%

Complex film of chitosan and carboxymethyl cellulose nanofibers

Kawasaki

Nakaji-Hirabayashi

Masuyama

et al. 2016

Colloids and Surfaces B: Biointerfaces

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A polymer film composed of a mixture of chitosan (Ch) and carboxymethyl cellulose sodium salt (CMC) nanofibers was deposited on a glass surface. The thin film of the Ch-CMC mixture obtained was stable, and fibroblast adhesion to the film was lowest when the weight ratio of Ch to CMC was 4:6. The ζ-potential and contact angle of the mixture film indicated that a polyion complex of Ch and CMC was formed. The mechanical strength of the film composed of Ch-CMC nanofiber complexes was much higher than that of the film composed of Ch-water-soluble CMC complexes (non-nanofiber), likely because the entanglement of nanofibers was enhanced by electrostatic attractions. These results indicate that the charge-neutralized nanofiber film was highly effective in suppressing cell adhesion and therefore is a promising material for biomedical applications.

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Structure of water at zwitterionic copolymer film–liquid water interfaces as examined by the sum frequency generation method

Cited by 43 publications

References 41 publications

Optimization of the composition of zwitterionic copolymers for the easy-construction of bio-inactive surfaces

Optimization of the composition of zwitterionic copolymers for the easy-construction of bio-inactive surfaces

Adhesion and Proliferation of Human Periodontal Ligament Cells on Poly(2-methoxyethyl acrylate)

Complex film of chitosan and carboxymethyl cellulose nanofibers

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