Structure of type II collagen from sturgeon cartilage and its effect on adjuvant-induced rheumatoid arthritis in rats

Li, Zhaoxia; Bai, Xue; Fan, Yan; Jia, Qian‐Nan; Zhang, Hongwei; Hou, Hu

doi:10.1039/d1fo03929f

Cited by 10 publications

(1 citation statement)

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“…In this sense, several active substances such as polysaccharide, protein (especially collagen), and peptides were previously obtained from the cartilage of marine organisms, such as sharks, sturgeon, rays and stingrays. For instance, many recent studies reported the potential availability of cartilages for extraction of chondroitin sulfate from shark [ 1 , 2 ], antioxidant collagen peptides from Siberian sturgeon ( Acipenser baerii ) [ 3 ], type II collagen from skate and sturgeon [ 4 , 5 ], gelatin from Siberian sturgeon ( Acipenser baerii ) [ 6 ], collagenous polypeptide (37 kDa) prepared from whale shark cartilage ( Rhincodon typus ) [ 7 ] and low-molecular-weight protein from shark [ 8 ]. Among these biomolecules, collagen is the most abundant fibrous structural protein in animals, accounting for approximately 30% of the total protein of the human body [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Biochemical Properties of Type II Collagen from Blue Shark (Prionace glauca) Cartilage

Pan

Wei

et al. 2023

Marine Drugs

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Numerous studies have shown that type II collagen (CII) has a potential role in the treatment of rheumatoid arthritis. However, most of the current studies have used terrestrial animal cartilage as a source of CII extraction, with fewer studies involving marine organisms. Based on this background, collagen (BSCII) was isolated from blue shark (Prionace glauca) cartilage by pepsin hydrolysis and its biochemical properties including protein pattern, total sugar content, microstructure, amino acid composition, spectral characteristics and thermal stability were further investigated in the present study. The SDS-PAGE results confirmed the typical characteristic of CII, comprising three identical α1 chains and its dimeric β chain. BSCII had the fibrous microstructure typical of collagen and an amino acid composition represented by high glycine content. BSCII had the typical UV and FTIR spectral characteristics of collagen. Further analysis revealed that BSCII had a high purity, while its secondary structure comprised 26.98% of β-sheet, 35.60% of β-turn, 37.41% of the random coil and no α-helix. CD spectra showed the triple helical structure of BSCII. The total sugar content, denaturation temperature and melting temperature of BSCII were (4.20 ± 0.03)%, 42 °C and 49 °C, respectively. SEM and AFM images confirmed a fibrillar and porous structure of collagen and denser fibrous bundles formed at higher concentrations. Overall, CII was successfully extracted from blue shark cartilage in the present study, and its molecular structure was intact. Therefore, blue shark cartilage could serve as a potential source for CII extraction with applications in biomedicine.

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Section: Introductionmentioning

confidence: 99%

Isolation and Biochemical Properties of Type II Collagen from Blue Shark (Prionace glauca) Cartilage

Pan

Wei

et al. 2023

Marine Drugs

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Isolation and Characterization of Marine-Derived Collagens and Gelatins in the Perspective of Biomedical Application

Alves

Marques

Almeida

et al. 2023

Handbook of the Extracellular Matrix

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Isolation and Characterization of Marine-Derived Collagens and Gelatins in the Perspective of Biomedical Application

Alves,

Marques,

Almeida

et al. 2024

Handbook of the Extracellular Matrix

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Structure of type II collagen from sturgeon cartilage and its effect on adjuvant-induced rheumatoid arthritis in rats

Abstract: The purpose of this paper was to extract and characterize type II collagen of sturgeon cartilage (SC-CII), and to explore the effects of taking SC-CII orally on rheumatoid arthritis (RA)...

Cited by 10 publications

References 64 publications

Isolation and Biochemical Properties of Type II Collagen from Blue Shark (Prionace glauca) Cartilage

Isolation and Biochemical Properties of Type II Collagen from Blue Shark (Prionace glauca) Cartilage

Isolation and Characterization of Marine-Derived Collagens and Gelatins in the Perspective of Biomedical Application

Isolation and Characterization of Marine-Derived Collagens and Gelatins in the Perspective of Biomedical Application

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