Structure of the Sertoli Cell

Hess, Rex A.; França, Luiz R.

doi:10.1016/b978-012647751-1/50004-0

Cited by 40 publications

(35 citation statements)

References 116 publications

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“…The vacuoles were surrounded by membrane and sometimes contained multivesicular body-like structures inside (data not shown). The vacuolar membranes were often lined by actin filament bundles with endoplasmic reticulum cisternae located beneath them (Fig 3B); this structure is characteristic of the adhesion complexes that attach germ cells to the seminiferous epithelium and suggests that the vacuoles reflect germ cell loss (Hellsten et al, 2002; Hess and Franca, 2005). Another morphologic abnormality evident in the Sertoli cells of Gata4 cKO mice was the accumulation of large lipid droplets (Fig 3A), which has been reported to accompany germ cell destruction (Setchell and Breed, 2006).…”

Section: Resultsmentioning

confidence: 99%

GATA4 regulates Sertoli cell function and fertility in adult male mice☆

Kyrönlahti

Euler

Bielińska

et al. 2011

Molecular and Cellular Endocrinology

101

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Transcription factor GATA4 is expressed in Sertoli and Leydig cells and is required for proper development of the murine fetal testis. The role of GATA4 in adult testicular function, however, has remained unclear due to prenatal lethality of mice harboring homozygous mutations in Gata4. To characterize the function of GATA4 in the adult testis, we generated mice in which Gata4 was conditionally deleted in Sertoli cells using Cre-LoxP recombination with Amhr2-Cre. Conditional knockout (cKO) mice developed age-dependent testicular atrophy and loss of fertility, which coincided with decreases in the quantity and motility of sperm. Histological analysis demonstrated Sertoli cell vacuolation, impaired spermatogenesis, and increased permeability of the blood-testis barrier. RT-PCR analysis of cKO testes showed decreased expression of germ cell markers and increased expression of testicular injury markers. Our findings support the premise that GATA4 is a key transcriptional regulator of Sertoli cell function in adult mice.

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Section: Resultsmentioning

confidence: 99%

GATA4 regulates Sertoli cell function and fertility in adult male mice☆

Kyrönlahti

Euler

Bielińska

et al. 2011

Molecular and Cellular Endocrinology

101

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“…Studies have illustrated that Sertoli cells, the somatic constituents of the seminiferous epithelium, also contribute to the epithelial cycle. For instance, Sertoli cells have been shown to assume various shapes throughout different stages of the epithelial cycle (Russell, 1993c;Vogl et al, 1993;Hess and Franca, 2005). In fact, it has been reported that as much as ϳ40% of the Sertoli cell surface is in contact with elongated spermatids alone (Russell et al, 1986;de França et al, 1993), illustrating that the interaction between the Sertoli cell and this germ cell type is enormous.…”

Section: A Cellular Organization Of the Seminiferous Epithelium In Tmentioning

confidence: 99%

Anchoring Junctions As Drug Targets: Role in Contraceptive Development

2008

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“…In conclusion, the age of appearance of α-SMA coincides with the onset of postnatal division of spermatogonia, and vimentin may play a key role in stabilizing Sertoli cell nuclei during their transformation in bovine. positioning of intracellular organelles, forming of cytoplasmic extensions, and anchoring of organelles to the plasma membrane [1]. As a specific marker of smooth muscle differentiation [2], seminiferous peritubular α-smooth muscle actin (SMA) has been detected in the first few postnatal days in the rat testis [3,4] and in the early postnatal ovine testis [5].…”

mentioning

confidence: 99%

Postnatal Developmental Changes in Immunohistochemical Localization of .ALPHA.-Smooth Muscle Actin (SMA) and Vimentin in Bovine Testes

Devkota

Sasaki

Takahashi³

et al. 2006

Journal of Reproduction and Development

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Abstract. The present study demonstrates the postnatal developmental changes in immunohistochemical localization of α-smooth muscle actin (SMA) and vimentin in the bovine testis. In the peritubular myoid cells of seminiferous tubules and the sub-epithelial and stromal cells of straight tubules and the rete testis, α-SMA starts appearing at around 4 months of age. Peritubular α-SMA attains the continuous mature pattern at around 5 months of age whereas sub-epithelial and stromal α-SMA increases with advancing age. Vimentin is localized in the perinuclear zone of Sertoli cells, peritubular and vascular wall cells, a few interstitial cells, and in the basal part of the epithelia of straight and rete tubules. Developmental changes are only evident in the Sertoli cell vimentin, which is basal and weak at birth and increases moderately until 4 months of age. From around 5 to 8 months of age when the Sertoli cells are under morphological transformation, vimentin intensity is considerably increased and the characteristic vimentin extensions connect the Sertoli nuclei to the basal membrane. These extensions get shorter at around 9 month of age as the Sertoli nuclei are positioned basally. The mature Sertoli cell perinuclear vimentin is strong and stable without infranuclear extension. In conclusion, the age of appearance of α-SMA coincides with the onset of postnatal division of spermatogonia, and vimentin may play a key role in stabilizing Sertoli cell nuclei during their transformation in bovine. positioning of intracellular organelles, forming of cytoplasmic extensions, and anchoring of organelles to the plasma membrane [1]. As a specific marker of smooth muscle differentiation [2], seminiferous peritubular α-smooth muscle actin (SMA) has been detected in the first few postnatal days in the rat testis [3,4] and in the early postnatal ovine testis [5]. Increasing patterns of α-SMA in the ovine testis [5] and changes in the actin filament arrangement in the rat testis [6] during p o s t n a t a l d e v e l o p m e n t h a v e s u g g e s t e d a relationship for SMA with testicular development and functions. However, no postnatal localization patterns or possible functional relationships for SMA in the bovine testis have been reported so far.In the testis, Sertoli cells support germ cells and also constitute the seminiferous tubules. Sertoli cells posses a highly organized cytoskeleton, and vimentin, the most common intermediate filament [7,8], is located around the nucleus and provides it with structural support [5,[9][10][11]. During pubertal processes, mammalian Sertoli cells undergo transformation [12,13] that is likely related to cytoskeletal changes [11,14]. Increases in Sertoli cell vimentin in the prepubertal ovine testis [5] and changes of its distribution area during postnatal development in rats [11] have indicated vimentin activity during postnatal development. Although perinuclear localization of vimentin has been demonstrated in the pre-Sertoli and adult Sertoli cells of the bovine testis [10,15], its dist...

show abstract

Structure of the Sertoli Cell

Cited by 40 publications

References 116 publications

GATA4 regulates Sertoli cell function and fertility in adult male mice☆

GATA4 regulates Sertoli cell function and fertility in adult male mice☆

Anchoring Junctions As Drug Targets: Role in Contraceptive Development

Postnatal Developmental Changes in Immunohistochemical Localization of .ALPHA.-Smooth Muscle Actin (SMA) and Vimentin in Bovine Testes

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