Structure of the parotid gland in natural infection by Leishmania infantum in Canis familiaris

Fonseca, Clarisse Maria Barbosa; Mendonça, Tarsia Giabardo Silva; Pereira, Cristian Francisco de Carvalho; Barros, Gabriel Martins de; Silva, Andrezza Braga Soares da; Cavalcante, Maria Michele Araújo de Sousa; Cruz, Maria do Socorro Pires e; Júnior, Aírton Mendes Conde

doi:10.1016/j.archoralbio.2021.105077

Cited by 1 publication

(1 citation statement)

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“…Comparable to our results, these studies reported the enlargement of serous acini after feeding tannins to the animals and linked this enlargement to the higher secretory activity of acinar cells and the production of salivary proteins [ 42 , 43 ]. Enlargement of salivary gland acini seems to be a common response to external stimuli promoting higher production of saliva or salivary proteins, as shown in the case of Leishmania infantum infection in dogs [ 44 ]. Furthermore, we did not observe significant changes in the duct cell area, which was in line with previous studies, confirming the importance of acinar cells in response to a tannin-rich diet [ 41 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Salivary Gland Adaptation to Dietary Inclusion of Hydrolysable Tannins in Boars

Mavri

Čandek-Potokar

Fazarinc

et al. 2022

Animals

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The ingestion of hydrolysable tannins as a potential nutrient to reduce boar odor in entire males results in the significant enlargement of parotid glands (parotidomegaly). The objective of this study was to characterize the effects of different levels of hydrolysable tannins in the diet of fattening boars (n = 24) on salivary gland morphology and proline-rich protein (PRP) expression at the histological level. Four treatment groups of pigs (n = 6 per group) were fed either a control (T0) or experimental diet, where the T0 diet was supplemented with 1% (T1), 2% (T2), or 3% (T3) of the hydrolysable tannin-rich extract Farmatan®. After slaughter, the parotid and mandibular glands of the experimental pigs were harvested and dissected for staining using Goldner’s Trichrome method, and immunohistochemical studies with antibodies against PRPs. Morphometric analysis was performed on microtome sections of both salivary glands, to measure the acinar area, the lobular area, the area of the secretory ductal cells, and the sizes of glandular cells and their nuclei. Histological assessment revealed that significant parotidomegaly was only present in the T3 group, based on the presence of larger glandular lobules, acinar areas, and their higher nucleus to cytoplasm ratio. The immunohistochemical method, supported by color intensity measurements, indicated significant increases in basic PRPs (PRB2) in the T3 and acidic PRPs (PRH1/2) in the T1 groups. Tannin supplementation did not affect the histo-morphological properties of the mandibular gland. This study confirms that pigs can adapt to a tannin-rich diet by making structural changes in their parotid salivary gland, indicating its higher functional activity.

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