Structure of the O-specific polysaccharide from a marine bacterium Cellulophaga tyrosinoxydans

Tomshich, Svetlana V.; Kokoulin, Maxim S.; Kalinovsky, A. I.; Nedashkovskaya, Olga I.; Komandrova, Nadezhda A.

doi:10.1016/j.carres.2015.04.024

Cited by 5 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The nature and degree of purity were then evaluated by SDS‐PAGE followed by silver‐nitrate gel staining [18] . The gel displayed, for all three bacterial species, a ladder‐like pattern typical of an O‐antigen‐containing LPS (i. e., an S‐LPS) (not shown), which was in accordance with previous analyses [14–16] . A detailed compositional analysis was performed on the three LPSs to establish the fatty acid content (results are reported in Table S1).…”

Section: Resultssupporting

confidence: 76%

“…[18] The gel displayed, for all three bacterial species, a ladder-like pattern typical of an O-antigen-containing LPS (i. e., an S-LPS) (not shown), which was in accordance with previous analyses. [14][15][16] A detailed compositional analysis was performed on the three LPSs to establish the fatty acid content (results are reported in Table S1). This analysis disclosed an exceptionally heterogenous composition, which entailed also the presence of 2-hydroxylated acyl chains (i. e. 15 : 0(2-OH)), and that was qualitatively identical for C. baltica NNO 15840 T and C. tyrosinoxydans EM41 T while slightly differed for C. algicola ACAM 630 T .…”

Section: Isolation and Compositional Analysis Of The Lps From Cellulo...mentioning

confidence: 99%

“…Here, we focus on three species: C. algicola ACAM 630 T isolated from the surfaces of algal species from the Antarctic marine coast; [9] C. baltica NNO 15840 T isolated from the brown alga Fucus serratus inhabiting the North Sea of the Atlantic Ocean; [10] and C. tyrosinoxydans EM41 T isolated from seawater collected nearly the eastern coast of Jeju Island, Republic of Korea. [13] We have previously shown that these bacteria express an S-LPS of which we have characterized the O-antigen structure of C. algicola ACAM 630 T [14] and C. tyrosinoxydans EM41 T [15] whereas the one synthesized by C. baltica NNO 15840 T was established by Tomshich et al 2007. [16] This study focuses on the structural determination of the lipid A portion of the above Cellulophaga species, attained by combining data from chemical analyses and Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and tandem MS (MS/MS) investigation.…”

Section: Introductionmentioning

confidence: 96%

See 2 more Smart Citations

Pushing the Boundaries of Structural Heterogeneity with the Lipid A of Marine Bacteria Cellulophaga

et al. 2023

Self Cite

View full text Add to dashboard Cite

Marine bacteria, which are often described as chemical gold, are considered an exceptional source of new therapeutics. Considerable research interest has been given to lipopolysaccharides (LPSs), the main components of the Gram-negative outer membrane. LPS and its lipid A portion from marine bacteria are known to exhibit a tricky chemistry that has been often associated with intriguing properties such as behaving as immune adjuvants or anti-sepsis molecules. In this scenario, we report the structural determination of the lipid A from three marine bacteria within the Cellulophaga genus, which showed to produce an extremely heterogenous blend of tetra-to hexaacylated lipid A species, mostly carrying one phosphate and one D-mannose on the glucosamine disaccharide backbone. The ability of the three LPSs in activating TLR4 signaling revealed a weaker immunopotential by C. baltica NNO 15840 T and C. tyrosinoxydans EM41 T , while C. algicola ACAM 630 T behaved as a more potent TLR4 activator.

show abstract

Section: Resultssupporting

confidence: 76%

Section: Isolation and Compositional Analysis Of The Lps From Cellulo...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

Pushing the Boundaries of Structural Heterogeneity with the Lipid A of Marine Bacteria Cellulophaga

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is not clear, but the first published report of its existence was in 1959 from E. coli 30 . Since that time, there have been sporadic reports of the sugar being isolated from the O‐antigens of Hafnia alvei 1205, A. baumannii , Pseudomonas fluorescens , Cellulophaga tyrosinoxydans , and Aeromonas hydrophila 31–36 . An elegant biochemical characterization of the sugar N ‐acetyltransferase from E. coli O7 was described in 2007 37 .…”

Section: Discussionmentioning

confidence: 99%

“…30 Since that time, there have been sporadic reports of the sugar being isolated from the O-antigens of Hafnia alvei 1205, A. baumannii, Pseudomonas fluorescens, Cellulophaga tyrosinoxydans, and Aeromonas hydrophila. [31][32][33][34][35][36] An elegant biochemical characterization of the sugar N-acetyltransferase from E. coli O7 was described in 2007. 37 The kinetic parameters for the E. coli enzyme were similar to those reported here for VioB (K M of 0.14 mM and a k cat /K M of 1.1 Â 10 4 M À1 s À1 ), and interestingly, the Nacetyltransferase from E. coli demonstrated catalytic activity over a temperature range of 4 to 65 C.…”

Section: Production Of Dtdp-4-amino-46-dideoxy-dglucosementioning

confidence: 99%

Structure and function of an N‐acetyltransferase from the human pathogen Acinetobacter baumannii isolate BAL_212

2022

View full text Add to dashboard Cite

Acinetobacter baumannii is a Gram-negative bacterium commonly found in soil and water that can cause human infections of the blood, lungs, and urinary tract. Of particular concern is its prevalence in health-care settings where it can survive on surfaces and shared equipment for extended periods of time. The capsular polysaccharide surrounding the organism is known to be the major contributor to virulence. The structure of the K57 capsular polysaccharide produced by A. baumannii isolate BAL_212 from Vietnam was recently shown to contain the rare sugar 4-acetamido-4,6-dideoxy-D-glucose. Three enzymes are required for its biosynthesis, one of which is encoded by the gene H6W49_RS17300 and referred to as VioB, a putative N-acetyltransferase. Here, we describe a combined structural and functional analysis of VioB. Kinetic analyses show that the enzyme does, indeed, function on dTDP-4-amino-4,6-dideoxy-D-glucose with a catalytic efficiency of 3.9 x 10 4 M À1 s À1 (±6000), albeit at a reduced value compared to similar enzymes. Three high-resolution X-ray structures of various enzyme/ligand complexes were determined to resolutions of 1.65 Å or better. One of these models represents an intermediate analogue of the tetrahedral transition state. Differences between the VioB structure and those determined for the N-acetyltransferases from Campylobacter jejuni (PglD), Caulobacter crescentus (PerB), and Psychrobacter cryohalolentis (Pcryo_0637) are highlighted. Taken together, this investigation sheds new insight into the Type I sugar N-acetyltransferases. K E Y W O R D S 4,6-dideoxyhexoses, 4-acetamido-4,6-dideoxy-D-glucose, 4-amino-4,6-dideoxy-D-glucose, Acinetobacter baumannii, carbohydrate, enzyme structure, N-acetyltransferase 1 | INTRODUCTIONAcinetobacter baumannii is an opportunistic Gram-negative bacterium that has recently emerged as a significant human health threat worldwide. 1,2 Due to the increasing rise of A. baumannii infections in hospital settings, and its extensive antibiotic resistance spectrum, it has been designated as

show abstract

Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter

Liao

et al. 2016

Carbohydrate Polymers

View full text Add to dashboard Cite

Structure of the O-specific polysaccharide from a marine bacterium Cellulophaga tyrosinoxydans

Cited by 5 publications

References 17 publications

Pushing the Boundaries of Structural Heterogeneity with the Lipid A of Marine Bacteria Cellulophaga

Pushing the Boundaries of Structural Heterogeneity with the Lipid A of Marine Bacteria Cellulophaga

Structure and function of an N‐acetyltransferase from the human pathogen Acinetobacter baumannii isolate BAL_212

Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter

Contact Info

Product

Resources

About