Structure of the O-antigen of Francisella tularensis strain 15

Vinogradov, E. V.; Shashkov, Alexander S.; Knirel, Yuriy A.; Kochetkov, Nikolay K.; Tochtamysheva, Natalya V.; Averin, Sergey F.; Гончарова, О. В.; Khlebnikov, V. S.

doi:10.1016/0008-6215(91)80036-m

Cited by 80 publications

(79 citation statements)

References 10 publications

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“…holarctica LPS are identical, but the O-antigen of F. tularensis subsp. novicida is distinct (101,119,124,126). The repeating subunits of the F. tularensis subsp.…”

Section: Bacterial Structure Lipopolysaccharidementioning

confidence: 99%

Francisella tularensis:Taxonomy, Genetics, and Immunopathogenesis of a Potential Agent of Biowarfare

McLendon

Apicella

Allen

2006

Annu. Rev. Microbiol.

205

292

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Tularemia is a zoonosis of humans caused by infection with the facultative intracellular bacterium Francisella tularensis. Interest in F. tularensis has increased markedly in the past few years because of its potential use as an agent of bioterrorism. Five subspecies of this organism are found in the Northern hemisphere, but only F. tularensis subsp. tularensis and subsp. holarctica cause disease in humans. This review summarizes what is known about the pathogenesis of tularemia with a focus on bacterial surface components such as lipopolysaccharide and capsule as well as information obtained from the F. tularensis subsp. tularensis SCHU S4 genome. In particular, the mechanisms of action of recently identified virulence factors are discussed in the context of bacterial replication in macrophages and manipulation of the host inflammatory response. Throughout this report shared and unique features of F. tularensis subsp. tularensis, subsp. holarctica, and subsp. novicida are discussed.

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“…holarctica LPS are identical, but the O-antigen of F. tularensis subsp. novicida is distinct (101,119,124,126). The repeating subunits of the F. tularensis subsp.…”

Section: Bacterial Structure Lipopolysaccharidementioning

confidence: 99%

Francisella tularensis:Taxonomy, Genetics, and Immunopathogenesis of a Potential Agent of Biowarfare

McLendon

Apicella

Allen

2006

Annu. Rev. Microbiol.

205

292

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“…The illdefined capsule (34,60,67), lipopolysaccharide (LPS) (61,64,74), and the genes involved in intramacrophage growth (42,50,69) are among a few known factors that can contribute to pathogenesis and virulence of F. tularensis. The recently sequenced genomes of three F. tularensis strains have predicted approximately 2,000 genes (40,54), but many of the putative genes do not have assigned biological roles.…”

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confidence: 99%

Genome-Wide Identification ofFrancisella tularensisVirulence Determinants

et al. 2007

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Francisella tularensis is a gram-negative pathogen that causes life-threatening infections in humans and has potential for use as a biological weapon. The genetic basis of the F. tularensis virulence is poorly understood. This study screened a total of 3,936 transposon mutants of the live vaccine strain for infection in a mouse model of respiratory tularemia by signature-tagged mutagenesis. We identified 341 mutants attenuated for infection in the lungs. The transposon disruptions were mapped to 95 different genes, virtually all of which are also present in the genomes of other F. tularensis strains, including human pathogenic F. tularensis strain Schu S4. A small subset of these attenuated mutants carried insertions in the genes encoding previously known virulence factors, but the majority of the identified genes have not been previously linked to F. tularensis virulence. Among these are genes encoding putative membrane proteins, proteins associated with stress responses, metabolic proteins, transporter proteins, and proteins with unknown functions. Several attenuated mutants contained disruptions in a putative capsule locus which partially resembles the poly-␥-glutamate capsule biosynthesis locus of Bacillus anthracis, the anthrax agent. Deletional mutation analysis confirmed that this locus is essential for F. tularensis virulence.

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“…tularensis, F. tularensis subsp. holarctica, and Francisella novicida (4,15,(21)(22)(23). In parallel, the wbt gene cluster supposedly involved in LPS O-antigen biogenesis has been identified by in silico analysis of the F. tularensis subsp.…”

mentioning

confidence: 99%

Role of the wbt Locus of Francisella tularensis in Lipopolysaccharide O-Antigen Biogenesis and Pathogenicity

et al. 2007

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Francisella tularensis is a highly infectious bacterial pathogen, responsible for the zoonotic disease tularemia. We screened a bank of transposon insertion mutants of F. tularensis subsp. holarctica LVS for colony morphology alterations and selected a mutant with a transposon insertion in wbtA, the first gene of the predicted lipopolysaccharide O-antigen gene cluster. Inactivation of wbtA led to the complete loss of O antigen, conferred serum sensitivity, impaired intracellular replication, and severely attenuated virulence in the mouse model. Notably, this mutant afforded protection against a challenge against virulent LVS.

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Structure of the O-antigen of Francisella tularensis strain 15

Cited by 80 publications

References 10 publications

Francisella tularensis:Taxonomy, Genetics, and Immunopathogenesis of a Potential Agent of Biowarfare

Francisella tularensis:Taxonomy, Genetics, and Immunopathogenesis of a Potential Agent of Biowarfare

Genome-Wide Identification ofFrancisella tularensisVirulence Determinants

Role of the wbt Locus of Francisella tularensis in Lipopolysaccharide O-Antigen Biogenesis and Pathogenicity

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