Structure of the levan elaborated by Streptococcus salivarius strain 51: An application of chemical-ionisation mass-spectrometry

Hancock, R. A.; Marshall, Keith; Weigel, Helmut

doi:10.1016/s0008-6215(00)83152-3

Cited by 40 publications

(16 citation statements)

References 13 publications

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“…Where stud-VOL. 70,2006 GLUCANSUCRASE AND FRUCTANSUCRASE ENZYMES OF LAB 161 ied, this was corroborated by the the composition of the polymer synthesized by the LAB host (Table 1). An exception to this rule is the GS from L. mesenteroides NRRL B-1299 (DSRE), which carries an additional C-terminally located catalytic domain (17) ( Table 1).…”

Section: Structural and Functional Organization Of Glucansucrase Enzymesmentioning

confidence: 68%

“…In general, LAB produce two types of fructans using FS (see also Table 2): levans, consisting mainly of ␤-(236)-linked fructose residues, occasionally containing ␤-(231)-linked branches, and inulin-type fructans, with ␤-(231)-linked fructose residues, with ␤-(236)-linked branches. Levan production has been reported for streptococci (19,70,178), L. mesenteroides (156), L. reuteri 121 (208,210), and Lactobacillus sanfranciscensis (92,93). Lactobacillus frumenti, Lactobacillus pontis, Lactobacillus panis and Weissella confusa were also found to produce fructans, but their fructan binding types have not been determined (92,93,198).…”

Section: Reactions Catalyzed and Fructan Product Synthesismentioning

confidence: 99%

“…3): (i) a signal peptide, (ii) an N-terminal VOL. 70,2006 GLUCANSUCRASE AND FRUCTANSUCRASE ENZYMES OF LAB 167…”

Section: Structural and Functional Organization Of Fructansucrase Enzmentioning

confidence: 99%

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Structure-Function Relationships of Glucansucrase and Fructansucrase Enzymes from Lactic Acid Bacteria

Hijum

Kralj

Ozimek

et al. 2006

Microbiol Mol Biol Rev

392

315

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SUMMARY Lactic acid bacteria (LAB) employ sucrase-type enzymes to convert sucrose into homopolysaccharides consisting of either glucosyl units (glucans) or fructosyl units (fructans). The enzymes involved are labeled glucansucrases (GS) and fructansucrases (FS), respectively. The available molecular, biochemical, and structural information on sucrase genes and enzymes from various LAB and their fructan and α-glucan products is reviewed. The GS and FS enzymes are both glycoside hydrolase enzymes that act on the same substrate (sucrose) and catalyze (retaining) transglycosylation reactions that result in polysaccharide formation, but they possess completely different protein structures. GS enzymes (family GH70) are large multidomain proteins that occur exclusively in LAB. Their catalytic domain displays clear secondary-structure similarity with α-amylase enzymes (family GH13), with a predicted permuted (β/α)8 barrel structure for which detailed structural and mechanistic information is available. Emphasis now is on identification of residues and regions important for GS enzyme activity and product specificity (synthesis of α-glucans differing in glycosidic linkage type, degree and type of branching, glucan molecular mass, and solubility). FS enzymes (family GH68) occur in both gram-negative and gram-positive bacteria and synthesize β-fructan polymers with either β-(2→6) (inulin) or β-(2→1) (levan) glycosidic bonds. Recently, the first high-resolution three-dimensional structures have become available for FS (levansucrase) proteins, revealing a rare five-bladed β-propeller structure with a deep, negatively charged central pocket. Although these structures have provided detailed mechanistic insights, the structural features in FS enzymes dictating the synthesis of either β-(2→6) or β-(2→1) linkages, degree and type of branching, and fructan molecular mass remain to be identified.

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Section: Structural and Functional Organization Of Glucansucrase Enzymesmentioning

confidence: 68%

Section: Reactions Catalyzed and Fructan Product Synthesismentioning

confidence: 99%

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Structure-Function Relationships of Glucansucrase and Fructansucrase Enzymes from Lactic Acid Bacteria

Hijum

Kralj

Ozimek

et al. 2006

Microbiol Mol Biol Rev

392

315

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“…For lactic acid bacteria, fructan production by streptococci and several lactobacilli has been reported (Tieking et al, 2003). Streptococcus salivarius strains produce branched levan polymers [containing up to 30 % b(2R1) branches] (Ebisu et al, 1975;Hancock et al, 1976;Simms et al, 1990) whereas Streptococcus mutans JC-2 produces a fructan of the inulin type consisting mainly of b(2R1)-linked fructosyl units with 5 % b(2R6) branches (Rosell & Birkhed, 1974;Ebisu et al, 1975). Recently, inulosucrase genes from lactic acid bacteria were reported in L. reuteri 121 (van Hijum et al, 2002) and Leuconostoc citreum (Olivares-Illana et al, 2002.…”

Section: Introductionmentioning

confidence: 99%

Biochemical and molecular characterization of a levansucrase from Lactobacillus reuteri

et al. 2004

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“…Levan is a polysaccharide composed of D-fructofurarose units joined by p- 2,6 b0nds.l Though it may be found in plants? the levans of interest in this work are those obtained from the extracellular substance of the bacteria of dental plaque.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical parameters of partially hydrolyzed S. salivarius levan fractions

Stivala

Zweig

1981

Biopolymers

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SynopsisThe fractions obtained from the partially hydrolyzed branched Streptococcus saliuarius levan were examined in solution. Sedimentation coefficients, So, intrinsic viscosities, [o], weight-average molecular weights, uw, and radii of gyration were obtained from sedimentation velocity, viscosity, and light-scattering measurements. Double logarithmic plots of [o] vs zW and So vs au each yielded two linear segments intersecting at Xw = lo5. Hydrodynamic data suggest that fractions of aw > lo5 behave as compact spheres, whereas for uw < lo5, the particles are best characterized as linear random coils. Calculations based on theories of random coils and spheres support the above observations.

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Structure of the levan elaborated by Streptococcus salivarius strain 51: An application of chemical-ionisation mass-spectrometry

Cited by 40 publications

References 13 publications

Structure-Function Relationships of Glucansucrase and Fructansucrase Enzymes from Lactic Acid Bacteria

Structure-Function Relationships of Glucansucrase and Fructansucrase Enzymes from Lactic Acid Bacteria

Biochemical and molecular characterization of a levansucrase from Lactobacillus reuteri

Physicochemical parameters of partially hydrolyzed S. salivarius levan fractions

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