Structure of the human carboxypeptidase M gene. Identification of a proximal GC-rich promoter and a unique distal promoter that consists of repetitive elements

Structural organization of the human complexin 2 gene (CPLX2) and aspects of its functional activity

“…Analysis of the exon -intron structure of CPLX2 showed that the differing portion of the alternative transcript 5V -UTR is encoded by an additional exon located within intron II, 7 kb away from exon III. Transcripts differing in their 5V -UTR and coding for the same protein have been described for many genes (Fukada et al, 2003;Li et al, 2002;Schuur et al, 2001). In some cases, the expression of such types of alternative transcripts is tissue specific or depends on a particular stage of the cell cycle (Friday et al, 2003;Gong et al, 1999); alternative usage of 5Vleader regions may affect mRNA stability and translational efficiency (Ayoubi and van de Ven, 1996;Pesole et al, 2001;Hughes and Brady, 2005).…”

Differential usage of two promoters of the Broad-Complex gene in the silkworm, Bombyx mori

“…3) will be translated into the same protein. Transcripts coding for the same protein but differing in their 5 0 -UTR have been reported for many genes (Falkenberg et al, 2003;Li et al, 2002;Schuur et al, 2001). In some cases, the expression of such alternative transcripts is tissue-specific or depends on a particular stage of the cell cycle (Friday et al, 2003;Saleh et al, 2002;Tomassetti et al, 2003), and alternative usage of the upstream leader sequence may affect mRNA stability and translational efficiency (Alves deAlmeida et al, 2006;Pesole et al, 2001).…”

Effect of mutation of two critical glutamic acid residues on the activity and stability of human carboxypeptidase M and characterization of its signal for glycosylphosphatidylinositol anchoring

“…The original CPM cDNA clone (HP-CPM) obtained from a human placental library [7] contained only part of the putative signal peptide and lacked an ATG initiation codon. To obtain the complete 5h sequence, a CPM clone (HK-CPM) was isolated from a human kidney λgt 10 cDNA library as described in [22]. A 670 bp EcoRI-NcoI fragment from HK-CPM was ligated with the 1480 bp NcoI-EcoRI fragment from HP-CPM.…”