Structure of the human acyl-CoA:cholesterol acyltransferase-2 (ACAT-2) gene and its relation to dyslipidemia

Katsuren, Keisuke

doi:10.1016/s1388-1981(01)00106-8

Cited by 21 publications

(21 citation statements)

References 28 publications

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“…To investigate these questions, we examined the relationship between ACAT-2 gene mutations and dyslipidemia. Our previous study revealed that three single-nucleotide polymorphisms (SNPs) in the ACAT-2 gene (E14G, T254I and IVS7) were not closely associated with plasma concentrations of lipids (13). In this study, we identified another SNP (IVS1 -8G→C) in the ACAT-2 gene and examined the relationship between this SNP and plasma concentrations of lipids or apolipoproteins.…”

Section: Introductionmentioning

confidence: 85%

“…Multiple pairs of primers were synthesized to amplify each of the ACAT-2 exonic regions with intronic borders (13). PCR consisted of 35 cycles of denaturation at 94 °C for 30 sec, annealing at 55-60 °C for 30 sec and extension at 72 °C for 1 min in 50 µl of reaction mixture containing 1.5 mM MgCl2, 300 µM of each dNTP (Nippon Gene, Japan), 0.3 µM of each primer, and 2.5 U of Taq DNA polymerase (Nippon Gene, Japan).…”

Section: Dna Analysismentioning

confidence: 99%

“…The abundance and quality of DNA fragments were analyzed by electrophoresis on 1.2% agarose gel, followed by ethidium bromide staining and inspection under UV light. Direct sequencing of doublestrand DNA fragments of exons 1 through 15 was performed using the internal sequencing primers (13).…”

Section: Dna Analysismentioning

confidence: 99%

“…First, to amplify the exonic regions and the splice junction sites, PCR primers were chosen in intronic regions -25 to -100 bp from the intron-exon boundaries (13). The resulting PCR products were then analyzed by direct sequencing.…”

Section: Screening For Acat-2 Gene Mutationsmentioning

confidence: 99%

See 3 more Smart Citations

Effects of a New Single-Nucleotide Polymorphism in the Acyl-CoA:Cholesterol Acyltransferase-2 Gene on Plasma Lipids and Apolipoproteins in Patients with Hyperlipidemia

Katsuren

Fukuyama

Takata

et al. 2003

JAT

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Section: Introductionmentioning

confidence: 85%

Section: Dna Analysismentioning

confidence: 99%

Section: Dna Analysismentioning

confidence: 99%

Section: Screening For Acat-2 Gene Mutationsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of a New Single-Nucleotide Polymorphism in the Acyl-CoA:Cholesterol Acyltransferase-2 Gene on Plasma Lipids and Apolipoproteins in Patients with Hyperlipidemia

Katsuren

Fukuyama

Takata

et al. 2003

JAT

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“…In the liver, ACAT catalyzes the intracellular cholesteryl ester production from esterification of cholesterol and is involved in the production and release of apoB-containing lipoproteins (34). Higher ACAT activity speeds up the release of VLDL from the liver, leading to higher blood cholesterol levels.…”

mentioning

confidence: 99%

Differential blood lipid-lowering effects of alkylsulfonated chitosan of different molecular weights in Syrian hamsters in vivo

Lin²,

Chen

et al. 2011

Mol Med Report

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Abstract. This study investigated the effects of alkylsulfonated chitosan of different molecular weights on intestinal lipid absorption, blood lipid profiles and circulating adhesion molecules. Syrian hamsters were fed an AIN-93G-based high-fat diet (HFD) and were orally administered 5 or 10 mg/kg BW of oligomer (6 kDa) chitosan (OC), low-molecular-weight (70 kDa) chitosan (LMC) or high-molecular-weight (200 kDa) chitosan (HMC) four times per week for 12 weeks. Animals receiving 2.5 mg/kg BW lovastatin (LOVA) served as a positive control. The blood lipid profiles of these control animals revealed that all chitosans and LOVA significantly decreased total triglyceride, total cholesterol, low-density lipoprotein (LDL)-cholesterol and very-low-density lipoprotein (VLDL)-cholesterol levels in a dose-dependent manner compared to the HFD-fed controls (P<0.05). The blood lipid lowering effectiveness of the three chitosans followed the order of LMC>OC>HMC. Hamsters receiving 5 and 10 mg/kg LMC (P<0.05) exhibited an increase in fecal fat content. Immunoblot assay revealed that acyl-coenzyme A:cholesterol acyltransferase-2 (ACAT-2) expression was suppressed in all chitosan-fed animals compared to the HFD-fed controls (P<0.05). These results suggest that chitosan effectively decreases blood lipid content, and its effectiveness depends on the molecular size of chitosan. The hypolipidemic effect of chitosan is partly attributed to its suppression of intestinal lipid absorption and hepatic ACAT-2 expression.

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Effects of oleanolic acid and maslinic acid on hyperlipidemia

Liu

Sun

Wang

et al. 2007

Drug Development Research

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In the present study, we examined the therapeutic effects of pentacyclic triterpenes, oleanolic acid (OA) and maslinic acid (MA), on hyperlipidemia in a high-cholesterol diet model. Hyperlipidemia was induced in male Sprague-Dawley rats by feeding with a high-cholesterol diet (HCD) for 30 days. MA and OA were supplemented (100 mg/kg body wt/day) during the last 15 days. The levels of serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C), and lowdensity lipoprotein-cholesterol (LDL-C) increased in hyperlipidemic rats. An apparent increase in the expression of Acyl-CoA cholesterol acyltransferase (ACAT) mRNA was seen in HCD-fed rats. The activities of hepatic marker enzymes that serve as indices of cellular injury were also altered in HCD-fed rats. Treatment with triterpenes modulated the abnormalities induced by hyperlipidimia. Lipid accumulation was decreased in histological findings. Elevated hepatic glycogen content (Po0.05) in triterpene groups was observed compared with HCD-fed groups. Furthermore, ACAT gene expression was suppressed compared with hyperlipidemia-induced groups without triterpenes. It can be concluded that triterpene treatment possesses therapeutic effects on diet-induced hyperlipidemia by inhibiting the intestinal absorption and storage of cholesterol. Drug Dev Res 68: 261-266, 2007. r 2007

show abstract

Structure of the human acyl-CoA:cholesterol acyltransferase-2 (ACAT-2) gene and its relation to dyslipidemia

Cited by 21 publications

References 28 publications

Effects of a New Single-Nucleotide Polymorphism in the Acyl-CoA:Cholesterol Acyltransferase-2 Gene on Plasma Lipids and Apolipoproteins in Patients with Hyperlipidemia

Effects of a New Single-Nucleotide Polymorphism in the Acyl-CoA:Cholesterol Acyltransferase-2 Gene on Plasma Lipids and Apolipoproteins in Patients with Hyperlipidemia

Differential blood lipid-lowering effects of alkylsulfonated chitosan of different molecular weights in Syrian hamsters in vivo

Effects of oleanolic acid and maslinic acid on hyperlipidemia

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