Structure of the human 5-HT7 receptor gene and characterization of its promoter region

Laenen, Koen; Haegeman, Guy; Vanhoenacker, Peter

doi:10.1016/j.gene.2007.01.003

Cited by 7 publications

(5 citation statements)

References 51 publications

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“…Recently, a linkage study has implicated 10q22‐q23 as a migraine susceptibility locus in a large sample set of 1675 individuals from 210 migraine families from Finland and Australia 13 . This is close to the location of human 5‐HT 7 receptor gene, which is located on chromosome 10q23.3‐q24.4 14 . This indicates a possible association between migraine and 5‐HT 7 receptor gene.…”

mentioning

confidence: 74%

Selective Inhibition of 5‐HT₇ Receptor Reduces CGRP Release in an Experimental Model for Migraine

Wang

et al. 2010

Headache

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confidence: 74%

Selective Inhibition of 5‐HT₇ Receptor Reduces CGRP Release in an Experimental Model for Migraine

Wang

et al. 2010

Headache

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“…5-hydroxytryptamine (serotonin) receptor 7 (HTR7) is a G protein-coupled receptor (GPCR) first described in 1993, and many transcription factors can bind to its promoter, such as AP2, EGR-1, and MAZ (6,7). HTR7 is associated with circadian rhythm, anxiety, depression, schizophrenia, nociception, and memory (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

HTR7 promotes laryngeal cancer growth through PI3K/AKT pathway activation

Sheng¹,

Liu²,

Lu³

et al. 2021

Ann Transl Med

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Background: Laryngeal cancer is a common malignancy of the head and neck, it's important to find novel targets for its therapy. The 5-hydroxytryptamine receptor 7 (HTR7) belongs to the G protein-coupled receptors (GPCRs) family which are easily druggable in diseases; however, its role in laryngeal cancer remains unknown. Methods: Colony formation assay, Soft agar growth assay, BrdU incorporation assay and MTT assay were used to analyze the effect of HTR7 on laryngeal cancer cell proliferation. Xenograft tumors in nude mice was used to analyze the effect of HTR7 on laryngeal cancer growth. Luciferase reporter assay was used to analyze the effect of HTR7 on phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT) pathway activity.Results: We found that HTR7 was significantly upregulated in laryngeal cancer tissues and cells, and patients with high HTR7 expression had shorter survival time than those with low HTR7 expression.Univariate and multivariate Cox regression models showed that HTR7 was an independent predictive factor for the prognosis of patients with laryngeal cancer. Cell proliferation assays and an animal model showed that HTR7 overexpression promoted laryngeal cancer proliferation and growth, while HTR7 knockdown inhibited laryngeal cancer proliferation and growth. Further analysis showed HTR7 activated the PI3K/AKT pathway, characterized by increased phosphorylation of AKT, luciferase reporter activity of forkhead box O (FOXO) factors, and target expression. Inhibition of the PI3K/AKT pathway in HTR7-overexpressing cells suppressed proliferation and growth, suggesting that HTR7 promotes laryngeal cancer proliferation and growth by activating the PI3K/AKT pathway.Conclusions: HTR7 is not only a target for laryngeal cancer therapy but also a prognostic factor for the prognosis of patients with laryngeal cancer.

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“…4×10 5 CHO cells were transiently transfected with JetPEI as described before (Laenen et al, 2007) in 6-well plates with 1 µg mouse 5-HT 7 receptor expression plasmid. 48 h after transfection, the cells were rinsed with ice-cold PBS (phosphate buffered saline) and harvested.…”

Section: Cell Culture and Transient Transfectionmentioning

confidence: 99%

Cloning, genomic organization and functionality of 5-HT7 receptor splice variants from mouse brain

Gellynck

Laenen

Andressen

et al. 2008

Gene

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Structure of the human 5-HT7 receptor gene and characterization of its promoter region

Cited by 7 publications

References 51 publications

Selective Inhibition of 5‐HT₇ Receptor Reduces CGRP Release in an Experimental Model for Migraine

Selective Inhibition of 5‐HT₇ Receptor Reduces CGRP Release in an Experimental Model for Migraine

HTR7 promotes laryngeal cancer growth through PI3K/AKT pathway activation

Cloning, genomic organization and functionality of 5-HT7 receptor splice variants from mouse brain

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Structure of the human 5-HT7 receptor gene and characterization of its promoter region

Cited by 7 publications

References 51 publications

Selective Inhibition of 5‐HT7 Receptor Reduces CGRP Release in an Experimental Model for Migraine

Selective Inhibition of 5‐HT7 Receptor Reduces CGRP Release in an Experimental Model for Migraine

HTR7 promotes laryngeal cancer growth through PI3K/AKT pathway activation

Cloning, genomic organization and functionality of 5-HT7 receptor splice variants from mouse brain

Contact Info

Product

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Selective Inhibition of 5‐HT₇ Receptor Reduces CGRP Release in an Experimental Model for Migraine

Selective Inhibition of 5‐HT₇ Receptor Reduces CGRP Release in an Experimental Model for Migraine