2018
DOI: 10.7554/elife.35720
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Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome

Abstract: ATP-dependent chromatin remodelling proteins represent a diverse family of proteins that share ATPase domains that are adapted to regulate protein–DNA interactions. Here, we present structures of the Saccharomyces cerevisiae Chd1 protein engaged with nucleosomes in the presence of the transition state mimic ADP-beryllium fluoride. The path of DNA strands through the ATPase domains indicates the presence of contacts conserved with single strand translocases and additional contacts with both strands that are uni… Show more

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Cited by 76 publications
(111 citation statements)
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“…The ATPase motor is in a closed, post-translocated state with AMP-PNP bound in the active site. A similar state was observed for Chd1 when bound to ADP·BeF 3 (Farnung et al, 2017;Sundaramoorthy et al, 2018Sundaramoorthy et al, , 2017. The double chromodomain is located at SHL +1 and contacts the nucleosomal DNA phosphate backbone via electrostatic interactions, in a fashion similar to that observed for S. cerevisiae Chd1 ( Fig.…”
Section: Resultssupporting
confidence: 77%
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“…The ATPase motor is in a closed, post-translocated state with AMP-PNP bound in the active site. A similar state was observed for Chd1 when bound to ADP·BeF 3 (Farnung et al, 2017;Sundaramoorthy et al, 2018Sundaramoorthy et al, , 2017. The double chromodomain is located at SHL +1 and contacts the nucleosomal DNA phosphate backbone via electrostatic interactions, in a fashion similar to that observed for S. cerevisiae Chd1 ( Fig.…”
Section: Resultssupporting
confidence: 77%
“…1). Binding at this location has also been observed for the chromatin remodellers Chd1 (Farnung et al, 2017;Sundaramoorthy et al, 2018), Snf2 (Liu et al, 2017), and Swr1 (Willhoft et al, 2018). The ATPase motor is in a closed, post-translocated state with AMP-PNP bound in the active site.…”
Section: Resultsmentioning
confidence: 71%
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