Structure of the Borrelia Bacteriophage φBB1 Procapsid

Rūmnieks, Jānis; Füzik, Tibor; Tārs, Kaspars

doi:10.1016/j.jmb.2023.168323

Cited by 3 publications

(2 citation statements)

References 64 publications

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“…burgdorferi strain CA-11.2A consistently produced ~10 times more phage than B31 (Fig 2A ) and was selected for further study. Imaging of purified virions collected from CA-11.2A by transmission electron microscopy reveals virions with an elongated capsid and contractile tail (Fig 2B), which is similar to the Myoviridae morphology of ϕBB-1 virions produced by strain B31 in vitro [9,42,43] and by a human B. burgdorferi isolate following ciprofloxacin treatment [44].…”

Section: Fbb-1 Phage Purification Virion Morphology and Proteomic Ana...mentioning

confidence: 70%

Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1

Faith,

Kinnersley,

Brooks

et al. 2024

PLoS Pathog

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Lyme disease is a tick-borne infection caused by the spirochete Borrelia (Borreliella) burgdorferi. Borrelia species have highly fragmented genomes composed of a linear chromosome and a constellation of linear and circular plasmids some of which are required throughout the enzootic cycle. Included in this plasmid repertoire by almost all Lyme disease spirochetes are the 32-kb circular plasmid cp32 prophages that are capable of lytic replication to produce infectious virions called ϕBB-1. While the B. burgdorferi genome contains evidence of horizontal transfer, the mechanisms of gene transfer between strains remain unclear. While we know that ϕBB-1 transduces cp32 and shuttle vector DNA during in vitro cultivation, the extent of ϕBB-1 DNA transfer is not clear. Herein, we use proteomics and long-read sequencing to further characterize ϕBB-1 virions. Our studies identified the cp32 pac region and revealed that ϕBB-1 packages linear cp32s via a headful mechanism with preferential packaging of plasmids containing the cp32 pac region. Additionally, we find ϕBB-1 packages fragments of the linear chromosome and full-length plasmids including lp54, cp26, and others. Furthermore, sequencing of ϕBB-1 packaged DNA allowed us to resolve the covalently closed hairpin telomeres for the linear B. burgdorferi chromosome and most linear plasmids in strain CA-11.2A. Collectively, our results shed light on the biology of the ubiquitous ϕBB-1 phage and further implicates ϕBB-1 in the generalized transduction of diverse genes and the maintenance of genetic diversity in Lyme disease spirochetes.

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Section: Fbb-1 Phage Purification Virion Morphology and Proteomic Ana...mentioning

confidence: 70%

Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1

Faith,

Kinnersley,

Brooks

et al. 2024

PLoS Pathog

View full text Add to dashboard Cite

show abstract

“…B. burgdorferi strain CA-11.2A consistently produced ∼10 times more phage than B31 ( Fig 2A ) and was selected for further study. Imaging of purified virions collected from CA-11.2A by transmission electron microscopy reveals virions with an elongated capsid and contractile tail ( Fig 2B ), which is similar to the Myoviridae morphology of ϕBB-1 virions produced by strain B31 in vitro [9, 40, 41] and by a human B. burgdorferi isolate following ciprofloxacin treatment [42].…”

Section: Resultsmentioning

confidence: 89%

Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1

Faith,

Kinnersley,

Brooks

et al. 2024

Preprint

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Lyme disease is a tick-borne infection caused by the spirocheteBorrelia(Borreliella)burgdorferi.Borreliaspecies have highly fragmented genomes composed of a linear chromosome and a constellation of linear and circular plasmids that encode diverse outer membrane lipoproteins, which facilitate movement of the spirochete between its tick vector and a vertebrate host in an enzootic cycle. TheB. burgdorferigenome shows evidence of horizontal transfer between strains, but the mechanisms remain poorly defined. Almost all Lyme disease spirochetes are infected by 32-kp circular plasmid (cp32) prophages that undergo lytic replication and produce infectious virions called ϕBB-1. In the laboratory, ϕBB-1 transduces cp32s and shuttle vectors between spirochetes. However, the extent that ϕBB-1 participates in horizontal gene transfer between Lyme disease spirochetes is not known. Here, we use proteomics and long-read sequencing to characterize ϕBB-1 virions and the genetic material they package. Our studies reveal that ϕBB-1 packages linear cp32s via a headful mechanism. We identify the cp32pacregion and show that plasmids containing the cp32pacregion are preferentially packaged into ϕBB-1 virions. Additionally, we find ϕBB-1 packages fragments of the linear chromosome and other plasmids including lp54, cp26, and others. Furthermore, sequencing of ϕBB-1 packaged DNA allowed us to resolve the covalently closed hairpin telomeres for the linearB. burgdorferichromosome and most linear plasmids in strain CA-11.2A. Collectively, our results shed light on the biology of the ubiquitous ϕBB-1 phage and further implicates ϕBB-1 in the generalized transduction of diverse genes between spirochetes and the maintenance of genetic diversity in Lyme disease spirochetes.

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Structure of the Portal Complex from Staphylococcus aureus Pathogenicity Island 1 Transducing Particles In Situ and In Isolation

Mukherjee,

Kizziah,

Hawkins

et al. 2024

Journal of Molecular Biology

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Structure of the Borrelia Bacteriophage φBB1 Procapsid

Cited by 3 publications

References 64 publications

Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1

Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1

Characterization and genomic analysis of the Lyme disease spirochete bacteriophage ϕBB-1

Structure of the Portal Complex from Staphylococcus aureus Pathogenicity Island 1 Transducing Particles In Situ and In Isolation

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