Structure of t cell antigen receptor β chain in synovial fluid cells from patients with rheumatoid arthritis

Keystone, Edward; Minden, Mark D.; Klock, R.; Poplonski, L; Zalcberg, John; Takadera, Tsuneo; Mak, T. W.

doi:10.1002/art.1780311213

Cited by 75 publications

(31 citation statements)

References 9 publications

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“…Direct sequencing of selected V-J combinations confirmed that these Gaussian distributions did not contain an expanded clonal specificity which exceeded 25% of a particular V-J combination and could therefore be detected by direct sequencing. In contrast, deviations from the Gaussian distribution and dominance of one particular CDR3 length was seen for 12 V,/-Jf3 combinations in the three patients (Fig. 4).…”

Section: Cd4+ T Cells (Upper Panel) and Preactivated Il-2r+mentioning

confidence: 81%

Dominant clonotypes in the repertoire of peripheral CD4+ T cells in rheumatoid arthritis.

Goronzy¹,

Bartz‐Bazzanella²,

Hu³

et al. 1994

J. Clin. Invest.

144

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Clonal expansion of T cell specificities in the synovial fluid of patients has been taken as evidence for a local stimulation of T cells. By studying the T cell receptor (TCR) repertoire of CD4± T cells in the synovial and peripheral blood compartments of patients with early rheumatoid arthritis (RA), we have identified clonally expanded CD4 + populations. Expanded clonotypes were present in the peripheral blood and the synovial fluid but were not preferentially accumulated in the joint. Dominant single clonotypes could not be isolated from CD4+ cells of HLA-DRB1 *04+ normal individuals. Clonal expansion involved several distinct clonotypes with a preference for Vf633, V1314', and VJ817'CD4+ T cells. A fraction of clonally related T cells expressed IL-2 receptors, indicating recent activation. The frequencies of clonally expanded V/317+CD4+ T cells fluctuated widely over a period of one year. Independent variations in the frequencies of two distinct clonotypes in the same patient indicated that different mechanisms, and not stimulation by a single arthritogenic antigen, were involved in clonal proliferation. These data support the concept that RA patients have a grossly imbalanced TCR repertoire. Clonal expansion may result from intrinsic defects in T cell generation and regulation.The dominance of expanded clonotypes in the periphery emphasizes the systemic nature of RA and suggests that T cell proliferation occurs outside of the joint. (J. Clin. Invest. 1994. 94

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Section: Cd4+ T Cells (Upper Panel) and Preactivated Il-2r+mentioning

confidence: 81%

Dominant clonotypes in the repertoire of peripheral CD4+ T cells in rheumatoid arthritis.

Goronzy¹,

Bartz‐Bazzanella²,

Hu³

et al. 1994

J. Clin. Invest.

144

View full text Add to dashboard Cite

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“…It has been proposed that mycobacterial antigens are important in RA since mycobacterial reactive clones have been isolated from synovia (31). However, synovial T cells do not always appear to be clonally restricted in their T-cell receptor usage and this argues against a single local antigen response (32)(33)(34)(35)(36)(37). Lymphocytes can also reach inflammatory sites in other ways: by recognition of inflammatory endothelium (26) or by chemotaxis toward factors released by activated cells.…”

Section: Discussionmentioning

confidence: 99%

Perforin and granzyme A expression identifying cytolytic lymphocytes in rheumatoid arthritis.

Griffiths

Alpert

Lambert

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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Lymphocytes from the synovial fluid of patients with rheumatoid arthritis were examined for the expression of granzyme A and perforin. Previous studies have demonstrated that the expression of these proteins, which are implicated as mediators of cytotoxicity, can be used to identify putative cytolytic lymphocytes in vivo. Twenty-two synovial fluid samples were analyzed by in situ hybridization and immunohistochemistry. In six patients receiving low doses of immunosuppressant, a population of granzyme A-and perforin-expressing lymphocytes could be identified. In contrast, lymphocytes from patients who were receiving high doses of immunosuppressant did not contain any granzyme A-or perforin-expressing lymphocytes. Synovial fluid lymphocytes from patients with osteoarthritis did not express either marker.

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“…Evidence of oligoclonality manifested by the presence of rearranged bands detected by Southern blotting was rarely found in T cells directly isolated from synovial fluid samples (16,17). Moreover, T cell clones established in culture conditions designed to allow outgrowth ofall cells rarely exhibited identical rearrangements (18,19).…”

Section: Introductionmentioning

confidence: 99%

T cell receptor V beta gene bias in rheumatoid arthritis.

Jenkins

Nikaein²,

Zimmermann³

et al. 1993

J. Clin. Invest.

115

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Polymerase chain reaction (PCR) technology was employed to examine peripheral blood and synovial T increased synovial expression of V/i14 was found, but only in the synovial fluid samples. Reduced expression of V#1, Vj64, V/i5.1, V/i10, V,816, and V/i19 was also observed in synovial T cells. These results indicate that biased V,8 gene utilization in different peripheral compartments of RA patients can be observed in unselected T cell populations, and are consistent with the conclusion that populations of T cells expressing these V/i gene products may be involved in the pathogenesis of the disease. (J. Clin. Invest. 1993. 92:2688-2701

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Structure of t cell antigen receptor β chain in synovial fluid cells from patients with rheumatoid arthritis

Cited by 75 publications

References 9 publications

Dominant clonotypes in the repertoire of peripheral CD4+ T cells in rheumatoid arthritis.

Dominant clonotypes in the repertoire of peripheral CD4+ T cells in rheumatoid arthritis.

Perforin and granzyme A expression identifying cytolytic lymphocytes in rheumatoid arthritis.

T cell receptor V beta gene bias in rheumatoid arthritis.

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