Structure of O-Polysaccharide and Lipid A of Pantoea Agglomerans 8488

Bulyhina, T.V.; Zdorovenko, Evelina L.; Varbanets, L.D.; Shashkov, Alexander S.; Kadykova, Alexandra A.; Knirel, Yuriy A.; Lushchak, Oleh

doi:10.3390/biom10050804

Cited by 6 publications

(5 citation statements)

References 31 publications

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“…For residues A and B, the chemical shifts of protons and carbon could be assigned with the aid of HSQC, COSY, TOCSY, and HMBC (as shown in Table 2 ). For residue A, the TOCSY peaks at δ 5.03/3.78, 5.03/3.94, 1.18/3.94, 1.18/4.36, and 1.18/4.08 ppm, COSY peaks at δ 5.03/3.78, 3.94/3.78, 3.94/4.36, 4.08/4.36, and 4.08/1.18 ppm, and HMBC peaks at 3.94/68.94, 3.94/79.66, 3.78/76.06, 4.08/79.66, and 1.18/66.68 ppm identified residue A as →3)-α-L-Fucp(4SO 4 2− )-(1→, according to the results of the methylation analysis and other literature [ 36 , 37 ]. For residue B, the cross-peaks at δH 5.22/4.02, 5.22/3.89, 5.22/3.72, 4.02/3.89, and 4.02/3.79 ppm in the TOCSY spectrum, along with the cross-peaks at δH 5.22/3.72, 3.72/3.89, 3.89/3.79, 4.02/3.79, and 4.02/1.24 ppm in the COSY spectrum, indicated H1/C1-H6/C6 signals of residue B.…”

Section: Resultsmentioning

confidence: 63%

Fuc-S—A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism

et al. 2022

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Scope: The dysbiosis of intestinal microecology plays an important pathogenic role in the development of inflammatory bowel disease. Methods and Results: A polysaccharide named Fuc-S, with a molecular weight of 156 kDa, was prepared by the ultrasonic degradation of fucoidan. Monosaccharide composition, FTIR, methylation, and NMR spectral analysis indicated that Fuc-S may have a backbone consisting of →3)-α-L-Fucp-(1→, →4)-α-L-Fucp-(1→ and →3, 4)-α-D-Glcp-(1→. Moreover, male C57BL/6 mice were fed three cycles of 1.8% dextran sulfate sodium (DSS) for 5 days and then water for 7 days to induce colitis. The longitudinal microbiome alterations were evaluated using 16S amplicon sequencing. In vivo assays showed that Fuc-S significantly improved clinical manifestations, colon shortening, colon injury, and colonic inflammatory cell infiltration associated with DSS-induced chronic colitis in mice. Further studies revealed that these beneficial effects were associated with the inhibition of Akt, p-38, ERK, and JNK phosphorylation in the colon tissues, regulating the structure and abundance of the gut microbiota, and modulating the host–microbe tryptophan metabolism of the mice with chronic colitis. Conclusion: Our data confirmed the presence of glucose in the backbone of fucoidan and provided useful information that Fuc-S can be applied as an effective functional food and pharmaceutical candidate for IBD treatment.

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Section: Resultsmentioning

confidence: 63%

Fuc-S—A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism

et al. 2022

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“…The core oligosaccharide of P. agglomerans LPS is highly diversified. In the case of several strains described, it is similar to that of E. coli in that it contains glucose in the core oligosaccharide (Karamanos et al, 1992 ; Lerouge and Vanderleyden, 2002 ; Kohchi et al, 2006 ; Hashimoto et al, 2017 ; Varbanets et al, 2019 ; Bulyhina et al, 2020 ). Therefore, it was unsurprising that P1 adsorption to P. agglomerans L15 cells was possible and similar to that of E. coli K-12 cells concerning time and efficiency ( Supplementary Figure 1 ).…”

Section: Discussionmentioning

confidence: 82%

Interaction of bacteriophage P1 with an epiphytic Pantoea agglomerans strain—the role of the interplay between various mobilome elements

Giermasińska-Buczek,

Gawor,

Stefańczyk

et al. 2024

Front. Microbiol.

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P1 is a model, temperate bacteriophage of the 94 kb genome. It can lysogenize representatives of the Enterobacterales order. In lysogens, it is maintained as a plasmid. We tested P1 interactions with the biocontrol P. agglomerans L15 strain to explore the utility of P1 in P. agglomerans genome engineering. A P1 derivative carrying the Tn9 (cmR) transposon could transfer a plasmid from Escherichia coli to the L15 cells. The L15 cells infected with this derivative formed chloramphenicol-resistant colonies. They could grow in a liquid medium with chloramphenicol after adaptation and did not contain prophage P1 but the chromosomally inserted cmR marker of P1 Tn9 (cat). The insertions were accompanied by various rearrangements upstream of the Tn9 cat gene promoter and the loss of IS1 (IS1L) from the corresponding region. Sequence analysis of the L15 strain genome revealed a chromosome and three plasmids of 0.58, 0.18, and 0.07 Mb. The largest and the smallest plasmid appeared to encode partition and replication incompatibility determinants similar to those of prophage P1, respectively. In the L15 derivatives cured of the largest plasmid, P1 with Tn9 could not replace the smallest plasmid even if selected. However, it could replace the smallest and the largest plasmid of L15 if its Tn9 IS1L sequence driving the Tn9 mobility was inactivated or if it was enriched with an immobile kanamycin resistance marker. Moreover, it could develop lytically in the L15 derivatives cured of both these plasmids. Clearly, under conditions of selection for P1, the mobility of the P1 selective marker determines whether or not the incoming P1 can outcompete the incompatible L15 resident plasmids. Our results demonstrate that P. agglomerans can serve as a host for bacteriophage P1 and can be engineered with the help of this phage. They also provide an example of how antibiotics can modify the outcome of horizontal gene transfer in natural environments. Numerous plasmids of Pantoea strains appear to contain determinants of replication or partition incompatibility with P1. Therefore, P1 with an immobile selective marker may be a tool of choice in curing these strains from the respective plasmids to facilitate their functional analysis.

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“…In the present study, we report the in vitro antiviral activities and interaction of LPS and its structural components with TMV, since the effect of the product "Gaupsin" on the antiviral activity were previously shown [10,11]. LPS from nine strains of the phytopathogenic phytopathogenic bacterium P. agglomerans were isolated and chemically characterized by us earlier [17][18][19][20][21][22][23]. The individual structural components of the LPS macromolecule was obtained by mild acid hydrolysis, in which insoluble lipid A and water-soluble O-specific polysaccharide (OPS) and core oligosaccharide (OG-core) fractions were obtained.…”

Section: Methodsmentioning

confidence: 94%

“…To date, the antiviral activity of P. agglomerans LPS against phytopathogenic viruses has not been studied. Since the structures of OPS and lipids A of some of the studied P. agglomerans strains were established by us earlier [17,21], the purpose of our work was to study the antiviral activity of both LPS molecules and their individual structural components: OPS, OG-core and lipid A in vitro.…”

Section: F I G 3 Electron Micrographs Of the Tmv And Lps Structural Components Interactionsmentioning

confidence: 99%

“…The structure of the OPS of these strains was significantly different and presented by linear disaccharide: →3)-α-L-Rhap-(1→4)-α-D-Glcp-(1→ (P. agglomerans P324 [20]) or linear tetrasaccharide:→3)-α-L-Rhap-(1→6)-α-D-Manp-(1→3)-α-L-Fucp-(1→3)-β-D-GlcNAcp-(1→ (P. agglomerans 8488 [21]). This gives us grounds to assume that the peculiarities structure of the OPS don`t play a significant role in the effect of LPS on the infectivity of TMV in relation to the indicator plant.…”

Section: F I G 3 Electron Micrographs Of the Tmv And Lps Structural Components Interactionsmentioning

confidence: 99%

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Anti-TMV Activities of Pantoea agglomerans Lipopolysaccharides in vitro

Bulyhina¹,

Kyrychenko²,

Kharchuk³

et al. 2021

Mikrobiol. Z.

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Today there are no antiviral drugs of chemical nature that can completely cure virus-infected plants. The fact that their effect is limited to minimizing the pathogenic effect of viruses motivates many researchers to look for alternatives. In recent years it has been shown that lipopolysaccharides (LPS) of some bacteria, in particular representatives of the Pseudomonas genus were active against Tobacco mosaic virus (TMV). Therefore, we were interested in the additional study of LPS of phytopathogenic bacteria Pantoea agglomerans as a possible drug acting as antiviral agent. The aim of current study was to evaluate the antiviral activities of LPS obtained from phytopathogenic bacteria P. agglomerans against TMV in vitro. Methods. The antiviral activity of LPS preparations was investigated in vitro and assessed according to the inhibition percentage towards the number of local lesions in Datura stramonium leaves. P. agglomerans LPS was isolated from dry bacterial mass by phenol-water method. LPS mild acid degradation allowed to separate O-specific polysaccharide (OPS) and lipid A, which structures were identified by us earlier. The analysis of TMV and LPS interactions was carried out using a JEM 1400 transmission electron microscope (Jeol, Japan) at an accelerating voltage of 80 kV. Results. The most active were LPS preparations from P. agglomerans P324 and 8488. In vitro inhibitory efficacies of TMV infection by these LPS preparations was 59 and 60% respectively. LPS preparations of P. agglomerans 7969, 7604 and 9637, on the contrary, were inactive. Comparative analysis of the antiviral activity of LPS structural components of two P. agglomerans P324 and 7604 strains showed that the greatest inhibitory effect on the infectivity of TMV was exhibited by P. agglomerans P324 lipid A, the antiviral activity of which practically did not differ from the activity of the LPS molecule (it was lower by 7%). At the same time, the inhibitory effect of P. agglomerans 7604 core oligosaccharide (OG-core) against TMV was slightly higher compared to the effect of the whole LPS molecule. It can be assumed that the OG-core stimulated the defense mechanisms of plants and prevented the development of viral infection. Electron microscopic dates have shown that P. agglomerans P324 LPS at the concentration of 1 mg/ml influenced on freely located virions in the control causing “sticking” thus forming dense clusters, complexes or “bundles” of the virus. The individual structural components of P. agglomerans P324 LPS (lipid A and OG-core) did not have the same effect as a whole molecule. Conclusions. The study of the antiviral activity of LPS in the model system TMV – Datura stramonium L. plants showed that the most active were LPS preparations of only two strains of P. agglomerans (P324 and 8488) while the other seven strains were inactive. Individual structural components: lipid A from P. agglomerans P324 and OG-core from P. agglomerans 7604 decreased the infectivity of TMV by 7 and 15% higher than the initial LPS molecule. According to electron microscopy data the virions sticked together forming the dense clusters in case of the direct LPS-virus contacting in vitro whereas in the control it was observed just a single free virus particles. A more detailed study of the effect of individual structural components will help to understand the regularities of the LPS structure effect on TMV infectivity.

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Structure of O-Polysaccharide and Lipid A of Pantoea Agglomerans 8488

Cited by 6 publications

References 31 publications

Fuc-S—A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism

Fuc-S—A New Ultrasonic Degraded Sulfated α-l-Fucooligosaccharide—Alleviates DSS-Inflicted Colitis through Reshaping Gut Microbiota and Modulating Host–Microbe Tryptophan Metabolism

Interaction of bacteriophage P1 with an epiphytic Pantoea agglomerans strain—the role of the interplay between various mobilome elements

Anti-TMV Activities of Pantoea agglomerans Lipopolysaccharides in vitro

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