Structure of cellulose Acetobacter xylinum

Klechkovskaya, V. V.; Baklagina, Yu. G.; Stepina, N. D.; Хрипунов, А. К.; Buffat, PA; Suvorova, Elena I.; Zanaveskina, I. S.; Ткаченко, А. С.; Gladchenko, S. V.

doi:10.1134/1.1612596

Cited by 31 publications

(20 citation statements)

References 18 publications

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“…A seven-to-eight-day static cultivation at 30-31 C produced cellulose pellicle having the thickness of 3-5 mm. 28 The yield of cellulose pellicles may be influenced by many factors, such as oxygen, temperature, and medium ingredients. 29 The harvested cellulose pellicles were purified by washing out the medium in DI water and then lysing bacterial cells with 0.1 mol/L sodium hydroxide at 80 C for 1 h. 30 Cellulose pellicles were then washed with DI water until a constant near neutral pH was achieved.…”

Section: Preparation Of Enzyme Solutionsmentioning

confidence: 99%

Integration of cellulases into bacterial cellulose: Toward bioabsorbable cellulose composites

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2011

J Biomed Mater Res

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Cellulose biodegradation resulting from enzymolysis generally occurs in nature rather than in the human body because of the absence of cellulose degrading enzymes. In order to achieve in-vivo degradation in human body for in-vivo tissue regeneration applications, we developed a bioaborbable bacterial cellulose (BBC) material, which integrates one or more cellulose degrading enzymes (cellulases), and demonstrated its degradability in vitro using buffers with pH values relevant to wound environments. We introduced a double lyophilizing process to retain the microstructure of the bacterial cellulose as well as the activity of embedded enzymes allowing for long-term storage of the material, which only requires hydration before use. Enzymes and their combinations have been examined to optimize the in-vitro degradation of the BBC material. In-vitro studies revealed that acidic cellulases from Trichoderma viride showed reasonable activity for pH values ranging from 4.5 to 6.0. A commercial cellulase (cellulase-5000) did not show good activity at pH 7.4, but its degrading ability increased when used in conjunction with a β-glucosidase from Bacillus subtilis or a β-glucosidase from Trichoderma sp. Given the harmless glucose product of the enzymatic degradation of cellulose, the BBC material may be ideal for many wound care and tissue engineering applications for the bioabsorbable purpose.

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Section: Preparation Of Enzyme Solutionsmentioning

confidence: 99%

Integration of cellulases into bacterial cellulose: Toward bioabsorbable cellulose composites

Catchmark

2011

J Biomed Mater Res

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“…[23,24]). Modification of the BC samples was carried out in an aqueous solution ($1.3 wt.%) of the polymer -thermochromic conductive polymer complex based on thiophene derivativespoly (3,4-ethylenedioxythiophene)-poly(4-styrenesulphonate)…”

Section: Objects and Methodsmentioning

confidence: 96%

Terahertz properties of bacterial cellulose films and its composite with conducting polymer PEDOT/PSS

et al. 2015

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“…The cellulose Acetobacter xylinum gel films were synthesized according to the known technique described in our previous papers [20,26] with the use of the VKM V-880 strain. The growth medium contained aqueous solutions of glucose (7.0 wt %), yeast extract (0.3 wt %), peptone (0.3 wt %), beer wort (30 vol %), and ethanol (3 vol %).…”

Section: Sample Preparation and Experimental Techniquementioning

confidence: 99%

“…The choice of the cellulose Acetobacter xylinum as a scaffold (despite the slow resorptivity) was motivated by the fact that the gel film formed during the static cultivation possesses a number of necessary properties. The cellulose Acetobacter xylinum gel film is formed by a network, which consists of nanosized microfibrillar crystalline ribbons and is capable of retaining a large amount of water (the ratio between the dry polymer and water is approximately equal to 1/100), has a sufficiently high mechanical strength, and possesses a specific surface area of no less than 500 m 2 /g [20]. In our work, we investigated the mechanism of formation of the composite through the aggregation of two biocompatible compounds in an aqueous suspension.…”

Section: Introductionmentioning

confidence: 99%

“…We also have a many years' experience in investigations into the properties of cellulose Acetobacter xylinum as a carrier matrix of different drugs for wound coatings. In our preclinical trials, different Russian drugs, such as Katapol, Poviargol, Selenopol, superoxide dismutase, and many others, impregnated into cellulose Acetobacter xylinum gel films, were tested with a positive effect [20][21][22]. With the use of cellulose Acetobacter xylinum and poly(acrylamide) hydrogels, we prepared an artificial cartilage, which surpasses the known analogs in the strength, flexibility, elasticity, and biocompatibility [23].…”

Section: Introductionmentioning

confidence: 99%

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Investigation of nanocomposites based on hydrated calcium phosphates and cellulose Acetobacter xylinum

et al. 2008

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Composites based on two biocompatible compounds, namely, inorganic hydrated calcium phosphates and organic microfibrillar ribbons of cellulose Acetobacter xylinum , are prepared by aggregation in an aqueous suspension. The influence of the structural organization of the hydroxyapatite and temperature-time conditions on the formation of the composite materials of different compositions is investigated. It is revealed that the composite materials are textured and retain the crystal structure of cellulose and the structure of initial hydrated calcium phosphates. The analysis of the crystal structures allows us to propose a model of the interaction between the mineral and organic components of the composite material. In the framework of the model, the interaction is provided through the formation of hydrogen bonds with the participation of hydroxyl groups and oxygen atoms of the phosphor group of the hydroxyapatite and primary OH groups located at the (-110) and (110) faces of cellulose nanocrystals.

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Structure of cellulose Acetobacter xylinum

Cited by 31 publications

References 18 publications

Integration of cellulases into bacterial cellulose: Toward bioabsorbable cellulose composites

Integration of cellulases into bacterial cellulose: Toward bioabsorbable cellulose composites

Terahertz properties of bacterial cellulose films and its composite with conducting polymer PEDOT/PSS

Investigation of nanocomposites based on hydrated calcium phosphates and cellulose Acetobacter xylinum

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