Structure of biomimetic casein micelles: Critical tests of the hydrophobic colloid and multivalent-binding models using recombinant deuterated and phosphorylated β-casein

Raynes, Jared K.; Mata, Jitendra; Wilde, Karyn L.; Carver, John A.; Kelly, Sharon M.; Holt, Carl

doi:10.1016/j.yjsbx.2024.100096

Cited by 2 publications

(1 citation statement)

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“…It should first be noted that casein micelles are quite complex and are not fully understood. There are several different models for the structure of casein micelles, all of which are plausible, but none of which is definitive. − The immediate purpose here is to show the new data resulting from the monitored dialysis as a means of providing complementary information to understand more about the micellar behavior and not to solve the structural problem. The interpretive model is used next to attempt a partial phenomenological conjecture of the data trends.…”

Section: Resultsmentioning

confidence: 99%

Dialysis Monitoring of Ionic Strength and Denaturant Effects, and Their Reversibility, for Various Classes of Macromolecules

Jarand,

McLeod,

Reed

2024

Biomacromolecules

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Monitoring membrane-mediated dialysis in real time with static and dynamic light scattering revealed distinctive differences, including reversibility/irreversibility, in the effects of ionic strength (NaCl) and the denaturant guanidine-HCl (Gd) on a synthetic polyelectrolyte and several types of biomacromolecules: protein, polysaccharide, and polyampholyte. Dialysis cycles against aqueous NaCl and Gd, and reverse back to the original aqueous solution, were monitored. The behavior of Na-polystyrenesulfonate was reversible and yielded a detailed polymer physics description. The biomacromolecules additionally showed hydrogen-bonding/ hydrophobic (HP) interactions. An interpretive model was developed that considers the interplay among polyelectrolyte, polyampholyte, and HP potential energies in determining the different associative, aggregative, and dissociative behaviors. NaCl isolated purely electrostatic effects, whereas Gd combined electrostatic and HP effects. Some macromolecules showed partially reversible behavior, and others were completely irreversible. The dialysis monitoring method should prove useful for investigating fundamental macromolecular and colloid properties and for drug formulation and stability optimization.

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