Structure of anguibactin, a unique plasmid-related bacterial siderophore from the fish pathogen Vibrio anguillarum

Jalal, M. A. F.; Hossain, M. B.; Helm, D. van der; Sanders-Loehr, Joann; Actis, Luis A.; Crosa, Jorge H.

doi:10.1021/ja00183a044

Cited by 158 publications

(99 citation statements)

References 2 publications

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“…The unusual complement of thiazoline and thiazolidine heterocyclic rings in pyochelin has not been observed in other siderophores. Among other known siderophores, only anguibactin (34), the siderophore produced by Vibrio anguillarum, possesses either of these heterocyclic rings (i.e., thiazoline); however, unlike pyochelin, anguibactin has both hydroxamate-and catecholate-chelating groups. Extended-polylinker pUC derivative 37 pUC21…”

mentioning

confidence: 99%

FptA, the Fe(III)-pyochelin receptor of Pseudomonas aeruginosa: a phenolate siderophore receptor homologous to hydroxamate siderophore receptors

1994

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The Pseudomonas aeruginosa siderophore pyochelin is structurally unique among siderophores and possesses neither hydroxamate- nor catecholate-chelating groups. The structural gene encoding the 75-kDa outer membrane Fe(III)-pyochelin receptor FptA has been isolated by plasmid rescue techniques and sequenced. The N-terminal amino acid sequence of the isolated FptA protein corresponded to that deduced from the nucleotide sequence of the fptA structural gene. The mature FptA protein has 682 amino acids and a molecular mass of 75,993 Da and has considerable overall homology with the hydroxamate siderophore receptors FpvA of P. aeruginosa, PupA and PupB of Pseudomonas putida, and FhuE of Escherichia coli. This observation indicates that homologies between siderophore receptors are an unreliable predictor of siderophore ligand class recognition by a given receptor. The fptA gene was strongly regulated by iron; fptA transcription was totally repressed by 30 microM FeCl3, as determined by Northern (RNA) blotting. The promoter of the fptA gene contained the sequence 5'-ATAATGATAAGCATTATC-3', which matches the consensus E. coli Fur-binding site at 17 of 18 positions. The -10 promoter region and transcriptional start site of the fptA gene reside within this Fur-binding site.

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confidence: 99%

FptA, the Fe(III)-pyochelin receptor of Pseudomonas aeruginosa: a phenolate siderophore receptor homologous to hydroxamate siderophore receptors

1994

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“…Yet many other siderophores have been isolated by marine bacteria in cultures which have yet to be observed in the environment, including marinobactins , aquachelins , loihichelins , ochrobactins , vibrioferrin , anguibactin [Jalal et al, 1989], vanchrobactin , petrobactin [Barbeau et al, 2002], and moanachelins . Why have these compounds not appeared in any marine surveys thus far?…”

Section: Siderophore Distributionsmentioning

confidence: 99%

Molecular determination of marine iron ligands by mass spectrometry

Boiteau¹

2016

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Abstract:Marine microbes produce a wide variety of metal binding organic ligands that regulate the solubility and availability of biologically important metals such as iron, copper, cobalt, and zinc.In marine environments where the availability of iron limits microbial growth and carbon fixation rates, the ability to access organically bound iron confers a competitive advantage. Thus, the compounds that microbes produced to acquire iron play an important role in biogeochemical carbon and metal cycling. However, the source, abundance, and identity of these compounds are poorly understood. To investigate these processes, sensitive methodologies were developed to gain a compound-specific window into marine iron speciation by combining trace metal clean sample collection and chromatography with inductively coupled plasma mass spectrometry (LC-ICPMS) and electrospray ionization mass spectrometry (LC-ESIMS). Coupled with isotope pattern assisted search algorithms, these tools provide a means to quantify and isolate specific iron binding ligands from seawater and marine cultures, identify them based on their mass and fragmentation spectra, and investigate their metal binding kinetics.Using these techniques, we investigated the distribution and diversity of marine iron binding ligands. In cultures, LC-ICPMS-ESIMS was used to identify new members of siderophore classes produced by marine cyanobacteria and heterotrophic bacteria, including synechobactins and marinobactins. Applications to natural seawater samples from the Pacific Ocean revealed a wide diversity of both known and novel metal compounds that are linked to specific nutrient regimes. Ferrioxamines B, E, and G were identified in productive coastal waters near California and Peru, in oligotrophic waters of the North and South Pacific Gyre, and in association with zooplankton grazers. Siderophore concentrations were up to five-fold higher in iron-deficient offshore waters (9pM) and were dominated by amphibactins, amphiphilic siderophores that partition into cell membranes. Furthermore, synechobactins were detected within nepheloid layers along the continental shelf. These siderophores reflect adaptations that impact dissolved iron bioavailability and thus have important consequences for marine ecosystem community structures and primary productivity. The ability to map and characterize these compounds has opened new opportunities to better understand mechanisms that link metals with the microbes that use them. 4Acknowledgements:

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“…In contrast, siderophores such as vibriobactin and anguibactin produced by Vibrio cholerae (Griffiths et al, 1984) and Vibrio anguillarum (Loehr, 1986;Jalal et al, 1989) …”

Section: Effect Of Temperature On the Growth And Siderophore Productionmentioning

confidence: 99%

Temperature dependent 2,3-dihydroxybenzoic acid production in Acinetobacter sp. B-W

Kim¹,

Lee²,

Yang³

2015

The Korean Journal of Microbiology

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A soil microorganism producing iron chelator (siderophore) under low iron stress (up to 2 μM of iron) was identified as Acinetobacter sp. B-W by 16S rDNA sequence analysis, biochemical-, physiological tests and morphological analysis using electron microscope. Catechol nature of siderophore was detected by Arnow test. Although optimal cell growth was identified at 36°C in iron-limited media, significant quantities of siderophore were produced only at 28°C. Biosynthesis of siderophore was strongly inhibited by growth at 36°C. Production of siderophore was completely inhibited by 10 μM FeCl3. Iron chelator produced from Acinetobacter sp. B-W was purified from supernatant using butanol extraction, Sephadex LH-20 column chromatography and HPLC. Purified sideropore was identified as 2,3-dihydroxybenzoic acid by HPLC, TLC and IR analysis.

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Structure of anguibactin, a unique plasmid-related bacterial siderophore from the fish pathogen Vibrio anguillarum

Cited by 158 publications

References 2 publications

FptA, the Fe(III)-pyochelin receptor of Pseudomonas aeruginosa: a phenolate siderophore receptor homologous to hydroxamate siderophore receptors

FptA, the Fe(III)-pyochelin receptor of Pseudomonas aeruginosa: a phenolate siderophore receptor homologous to hydroxamate siderophore receptors

Molecular determination of marine iron ligands by mass spectrometry

Temperature dependent 2,3-dihydroxybenzoic acid production in Acinetobacter sp. B-W

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