Structure of an anti-tumor polysaccharide from Angelica sinensis (Oliv.) Diels

Cao, Wei; Li, Xiaoqiang; Liu, Li; Yang, Tiehong; Li, Chen; Fan, Hui-Ting; Jia, Min; Lu, Zheng-Guang; Mei, Qibing

doi:10.1016/j.carbpol.2006.02.034

Cited by 118 publications

(49 citation statements)

References 51 publications

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“…Multiple polysaccharides have been extracted from the root of A. sinensis. Cao et al gained an arabinoglucan, which exhibited significant anti-tumor activity both in vitro and in vivo (Cao et al, 2006). Sun et al isolated a pectic polysaccharide and presented potential radio protective effect (Sun et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the transport and absorption of Angelica sinensis polysaccharide through gastrointestinal tract both in vitro and in vivo

et al. 2017

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To investigate the absorption and delivery of ASP in gastrointestinal (GI) tract, cASP was successfully synthesized by chemically modifying with succinic anhydride and then conjugating with a near infrared fluorescent dye Cy5.5. Then, the capacity of oral absorption of cASP was evaluated. The results demonstrated that cASP had low toxicity and no disruption on the integrity of cell membrane. The endocytosis of cASP into the epithelial cells was time- and energy-dependent, which was mediated by macropinocytosis pathway and clathrin- and caveolae (or lipid raft)-related routes. Otherwise, the actin filaments played a relatively weak role at the same time. The transport study illustrated that cASP could penetrate through the epithelial monolayer and mainly mediated by the same routes as that in the endocytosis experiment. Moreover, both in vitro Ussing chamber and in vivo ligated intestinal loops models indicated that cASP could be diffused through the mucus barriers and be absorbed in the whole small intestine. Finally, near-infrared fluorescence imaging presented that cASP could be absorbed and circulated into the blood, then distributed into various organs after oral administration. In conclusion, ASP could be absorbed after oral administration through endocytosis process mainly mediated by macropinocytosis pathway and clathrin- and caveolae (or lipid raft)-related routes, then be absorbed and circulated into blood. This study presents a comprehensive understanding of oral delivery of cASP, which will provide theoretical basis for the clinical application of ASP.

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Section: Introductionmentioning

confidence: 99%

Investigation of the transport and absorption of Angelica sinensis polysaccharide through gastrointestinal tract both in vitro and in vivo

et al. 2017

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“…The 1 H-NMR spectrum shows strong anomeric H-1 (δ 5.52, 5.29, 5.09 and 5.07 ppm) proton signals, indicating the α-configuration of AGP33 monomers [11]. As shown in the 13 C-NMR spectrum of AGP33, the resonances in the region of δ 97-101 ppm for C-1 can be attributed to the anomeric carbon atoms of pyranose, as the furan ring signals can be observed around δ 107-109 ppm [12]. Peaks presented at δ 101.02, 100.51, and 99.64 ppm correspond to C-1 of the 1Ñ3-α-D-Glcp, 1Ñ6-α-D-Glcp, and 1Ñ4-α-D-Galp residues, respectively.…”

Section: Methylation Analysis Of Bagp33mentioning

confidence: 83%

“…The AGP33 signals in the 13 C-NMR spectrum are summarized in Table 1, which are in good agreement with the BAGP33 methylation results. Other signals in the 13 C-NMR and 1 H-NMR spectra (Table 1) are assigned based on their correlation with correlated spectroscopy ( 1 H-1 H COSY) and heteronuclear single-quantum coherence (HSQC) experiments [6,12]. …”

Section: Methylation Analysis Of Bagp33mentioning

confidence: 99%

Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

et al. 2016

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Abstract:In this study, we aimed at characterizing the structure and the anticoagulant activity of a polysaccharide fraction (AGP33) isolated from the gonads of Haliotis discus hannai Ino. AGP33 was extracted by enzymatic hydrolysis and purified by ion-exchange and gel-filtration chromatography. The backbone fraction of AGP33 (BAGP33), which appeared to contain of mannose, glucose and galactose, was prepared by partial acid hydrolysis. According to methylation and nuclear magnetic resonance (NMR) spectroscopy, the backbone of AGP33 was identified as mainly consisting of 1Ñ3-linked, 1Ñ4-linked, and 1Ñ6-linked monosaccharides. AGP33 is a sulfated polysaccharide with sulfates occur at 3-O-and 4-O-positions. It prolonged thromboplastin time (APTT), thrombin time (TT) and prothrombin time (PT) compared to a saline control solution in a dosage-dependent manner. AGP33 exhibited an extension (p < 0.01) of APTT compared to the saline group at concentrations higher than 5 µg/mL. AGP33 exhibited higher anticoagulant activity than its desulfated product (AGP33-des) and BAGP33. The results showed that polysaccharide with higher molecular weight and sulfate content demonstrated greater anticoagulant activity.

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“…Furthermore, terminal ␤-Larabinofuranosides are found in many plant biopolymers (3)(4)(5)(6)(7)(8) and in yessotoxin from the dinoflagellate algae Protoceratium reticulatum (31,32). Because DUF1680 family members are conserved in many species of bacteria, actinomycetes, fungi, and plants, they are thought to play a role in the effective degradation of plant biopolymers as well as hydroxyproline-rich glycoproteins.…”

Section: Volume 289 • Number 8 • February 21 2014mentioning

confidence: 99%

“…Extensins and solanaceous lectins are members of the hydroxyproline (Hyp) 2 -rich glycoproteins that are widely observed in plant cell wall fractions. Furthermore, terminal ␤-Larabinofuranosyl residues have been found in arabinogalactan protein from the pollen of timothy grass (3), rhamnogalacturonan-II (4 -6), olive arabinan (7), arabinoglucan from Angelica sinensis (8), and tomato arabinoxyloglucan (9). However, despite the broad distribution of ␤-L-arabinofuranosyl residues in plant cells, the degradative enzyme ␤-L-arabinofuranosidase (EC 3.2.1.185) 3 has never been found.…”

mentioning

confidence: 99%

Characterization of a Novel β-l-Arabinofuranosidase in Bifidobacterium longum

Fujita

Takashi

Obuchi

et al. 2014

Journal of Biological Chemistry

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Background: ␤-L-Arabinofuranosyl linkages are found in many plant biopolymers, but the degradation enzyme has never been found. Results: A novel ␤-L-arabinofuranosidase was found in Bifidobacterium longum. Conclusion: ␤-L-Arabinofuranosidase plays a key role in Bifidobacterium longum for ␤-L-arabinooligosaccharide usage. Significance: The members of the DUF1680 family might be used for the degradation of plant biopolymers.

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Structure of an anti-tumor polysaccharide from Angelica sinensis (Oliv.) Diels

Cited by 118 publications

References 51 publications

Investigation of the transport and absorption of Angelica sinensis polysaccharide through gastrointestinal tract both in vitro and in vivo

Investigation of the transport and absorption of Angelica sinensis polysaccharide through gastrointestinal tract both in vitro and in vivo

Anticoagulant Activity and Structural Characterization of Polysaccharide from Abalone (Haliotis discus hannai Ino) Gonad

Characterization of a Novel β-l-Arabinofuranosidase in Bifidobacterium longum

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