Structure of allelic variants of subtype 5 of histone H1 in pea Pisum sativum L.

Abstract

The pea genome contains seven histone H1 genes encoding different subtypes. Previously, the DNA sequence of only one gene, His1, coding for the subtype H1-1, had been identified. We isolated a histone H1 allele from a pea genomic DNA library. Data from the electrophoretic mobility of the pea H1 subtypes and their N-bromosuccinimide cleavage products indicated that the newly isolated gene corresponded to the H1-5 subtype encoded by His5. We confirmed this result by sequencing the gene from three pea lines with H1-5 allelic variants of altered electrophoretic mobility. The allele of the slow H1-5 variant differed from the standard allele by a nucleotide substitution that caused the replacement of the positively charged lysine with asparagine in the DNA-interacting domain of the histone molecule. A temperature-related occurrence had previously been demonstrated for this H1-5 variant in a study on a worldwide collection of pea germplasm. The variant tended to occur at higher frequencies in geographic regions with a cold climate. The fast allelic variant of H1-5 displayed a deletion resulting in the loss of a duplicated pentapeptide in the C-terminal domain. Heredity (2005) 94, 582-588.

Allelic isoforms of the chicken and duck histone H1.a

“…The CTD of histone H1 is likely to be involved in competitive interaction with the chromatin and other nuclear proteins [25]. On the basis of these studies, it seems likely that the amino acid changes in the CTD region [26,27] may affect its secondary structure and thereby the interaction of the mutated H1 variant with its targets.…”

A shift in erythrocyte histone H1 complement following selection in quail (Coturnix japonica)

“…A specific role for the histone H1 allelic isoform might be assigned based on the cause and effect relationship between the appearance and/or disappearance of a given allele leading to the characteristic phenotypic effects (Kowalski and Palyga 2012a). The histone H1 allele frequencies were shown to correlate with growth dynamics (Kosterin et al 1994;Bogdanova et al 2007), accumulated temperature of vegetation period (Bogdanova et al 2005), and geographic distribution of plants (Berdnikov et al 1993;Dudnikov 2012), plumage colour in duck (Palyga et al 2000), and the effects of environmental pollution in hare (Lepus europaeus).…”

Phenotypic effect of substitution of allelic variants for a histone H1 subtype specific for growing tissues in the garden pea (Pisum sativum L.)

“…1). Earlier (Bogdanova et al 2005), we derived an empiric formula predicting an electrophorectic mobility of a protein in the acetic acid/urea system (Panyim and Chalkley 1969) based on the number of amino acid residues of the molecule and the number of positively charged residues. Using this formula we estimated electrophoretic mobility for the pea H1 histones available from public databases.…”

“…Amplification and sequencing of His7 alleles A cDNA sequence (L34578) for a pea histone H1 (Woo et al 1995) codes for a protein with predicted electrophoretic mobility that corresponds well to the H1-7 subtype (Bogdanova et al 2005). We designed primers matching upstream and downstream sequences adjacent to the coding region of the L34578 accession (5¢-CAACGGTTATTCCCTCTCCTC-3¢ and 5¢-GTA CAAAGAACACAAGATGTGTTTC-3¢).…”

“…In the garden pea (Pisum sativum L.), it is observed for all seven H1 subtypes, each encoded by a single gene (Kosterin et al 1994). Alleles of two subtypes, H1-1 and H1-5, have been sequenced (Berdnikov et al 2003;Bogdanova et al 2005).…”