“…Further, the N-and O-linked glycans covalently attached to human erythrocyte CD59 were characterized, and the full HPLC glycosylation profile compared with that of human platelet CD59 to explore the extent of cellspecific glycosylation. Molecular modeling, based on the solution structure of the protein (18) was used to explore the spatial relationships between the lipid bilayer, the protein, and the N-and O-linked glycans. Several possible roles for the heterogeneous array of glycans attached to CD59 were proposed, including orientation of the active site, protection from protease cleavage, and limiting interactions with other CD59 molecules and with the cell surface.…”