Structure of a polysaccharide from the seeds of Cassia tora. Part II. Partial acidic hydrolysis

Abstract

the usual method.l-4 The purified polysaccharide $34.5" in N-NaOH) was found to be homogeneous by fractional precipitation followed by hydrolysis and quantitative estimation of the component sugars in each fraction, zone electrophoresis, and acetylation followed by deacetylation. Quantitative estimation showed that D-galactopyranose, D-g~ucopyranose, D-mannopyranose, and D-xylopyranose units were present in the molar ratio 2 : 2 : 7 : 1. The methylated polysaccharide yielded 2,3,4,6-tetra-O-methyl-~-glucose, 2,3,4-tri-0-methyl-~-xylose, 2,3,4,6-tetra-O-methyl-D-galactose, 2,3,4-tri-0-methyl-~-glucose, 2,3,6-tri-Omet hyl-D-glucose, 2,3,6-tri-O-methyl-~-mannose, 2,3-di-0-methyl-D-mannose, and 2,3-di-O-methyl-~-glucose in the approximate molar ratios 1 : 4 : 8 : 1 : 1 : 20 : 8 : 5. Graded hydrolysis indicated that D-xylose was liberated first, followed by D-galactose, D-glucose, and ultimately D-mannose. We now report the results of partial acidic hydrolysis of the polysaccharide.The structures of various oligosaccharides isolated during partial acidic hydrolysis were assigned on the basis of complete acidic hydrolysis followed by chromatographic examination, equivalent weight determination by the alkaline hypoiodite method: enzymic hydrolysis followed by paper chromatographic examination, oxidation by periodate, partial acidic hydrolysis, methylation

Other Plant Polysaccharides

“…When D-Xylp groups are incorporated, the resulting highly complex heteropolysaccharide [(e.g., that from seeds of the legume Cassia torà (99)] acquires, in addition, some of the characteristics of both a xyloglucan and a galactomannan (see Section III).…”

“…as well as D-Gal/? groups were found in the interesting highly branched polysaccharide extracted from seeds of another leguminous plant, Cassia torà (99). Xylose was released first upon graded hydrolysis of the product ([a] D positive in alkali), which yielded a large number of oligosaccharides, the structures of which were determined.…”

CassiaSeed Gums: A Renewable Reservoir for Synthesizing High Performance Materials for Water Remediation

“…A variety of chemical constituents have been isolated from roots and different aerial parts of the plants belonging to this genus. 31], Cassia nodosa [32,33], Cassia ovata [34], Cassia obovata [35], Cassia occidentalis [36][37][38], Cassia pleurocarpa [39], Cassia podocarpa [40], Cassia pudibunda [41], Cassia renigera [42], Cassia reticulata [43], Cassia siamea [44^16], Cassia sophera [47], Cassia spectabilis [48,49], Cassia surattensis [50], Cassia saligna [51], and Cassia torn [52][53][54] have been reported to contain substantial amount of gums having a basic backbone skeleton of ß{\->4) linked mannose units to which a-galactosyl [11] or galactobiosyl residues [7] are glycosidically attached as side chains. Their seed endosperms contain a substantive amount of the galactomannan polysaccharides.…”

Processing Scale-Up of Sicklepod (Senna obtusifolia L.) Seed

“…Although the recent introduction of Round-Up-ready soybeans mitigates infestation of the beans by sicklepod, the latter is so prolific that even volunteer stands yield over 1000 lb of seed per acre. The composition of sicklepod seed has been reported to include anthraquinones 1−2%, fats 5−7%, proteins 14−19%, and carbohydrates 66−69% − . Sicklepod flour imported from India is already in the market as a hydrocolloid additive in pet foods because of its unique galactomannan ratio, which enhances flexibility in the range of formulation compositions ( and Nicholson, J. J., personal communication).…”