Structure of a cytotoxic T-lymphocyte-specific gene shows a strong homology to fibrinogen beta and gamma chains.

Abstract

Using a subtractive cDNA cloning method, we isolated a number of T-lymphocyte-specific genes. One of these genes, represented by the cDNA clone pT49, is expressed in cytotoxic T lymphocytes but not in helper T lymphocytes or B lymphocytes. The protein structure deduced from the nucleotide sequence showed a high degree of homology to fibrinogen 13 and y subunits. This might indicate that evolutionarily fibrinogen has its origin in lymphocytes. In spite of the strong homology of pT49 protein to the fibrinogen subunits, the positions of the introns in the pT49 gene are totally different from those of the fibrinogen genes.

Crystal structure of a recombinant α E C domain from human fibrinogen-420

“…In addition to all the other known ␣ E C domains in mammals and chicken and the equivalent domain in lamprey, putative carbohydrate-binding sites occur at this position in the Drosophila scabrous protein (27), in the lamprey ␥ chain (28), and in the mammalian fibrinogen-related protein known as PT49 (29,30). …”

The novel CD4+CD25+ regulatory T cell effector molecule fibrinogen-like protein 2 contributes to the outcome of murine fulminant viral hepatitis

“…[9][10][11] The fgl2 gene was first cloned by Koyama et al from cytotoxic T lymphocytes, and the encoded protein has been classified as a member of the fibrinogenrelated superfamily based on its homology to the ␤ and ␥ chains of fibrinogen. 26 Previously we reported that in addition to its role in innate immunity as a membraneassociated prothrombinase, FGL2 also may have an important role in adaptive immune responses, similar to other members of the fibrinogen-like family of proteins, which include tenascin and angiopoietin. 27 Recombinant FGL2 was shown previously to suppress T cell proliferation to alloantigens, anti-CD3/CD28 antibodies, and Con A.…”

MultiCoil: A program for predicting two‐and three‐stranded coiled coils

“…The construction of the twostranded coiled-coil database is discussed in Berger et al The three-stranded coiled-coil database was constructed from 42 sequences. The proteins in the three-stranded database have been characterized in the following references: laminins (families A, Bl, and B2 chains) (Pikkarainen et al, 1987(Pikkarainen et al, , 1988Sasaki et al, , 1988Monte11 & Goodman, 1988Hunter et al, 1989Hunter et al, , 1992Engel, 1992;Kallunki et al, 1992;Nomizu et al, 1992;Beck et al, 1993;Porter et al, 1993;Gerecke et al, 1994;Vuolteenaho et al, 1994;Wewer et al, 1994), fibrinogens (families a, j?, and y chains) (Chung et al, 1981(Chung et al, , 1983a(Chung et al, , 1983bCrabtree & Kant, 1982;Rixon et al, 1983;Crabtree et al, 1985;Strong et al, 1985;Bohonus et al, 1986;Koyama et al, 1987;Wang et al, 1989;Pastori et al, 1990;Weissbach & Grieninger, 1990;Pan & Doolittle, 1992), K. lactis heat shock factor (Peteranderl & Nelson, 1992), S. cerevisiae heat shock transcription factor (Sorger & Nelson, 1989), influenza virus hemagglutinin (Bullough et al, 1994), Moloney murine leukemia virus ( Fass et al, 1996), fibritin encoded by Wac gene for bacteriophage T4 and K3 (Efimov et al, 1994), gp17 leg protein in bacteriophages T7 and T3, and macrophage scavenger receptor protein (Conway & Parry, 1991).…”