Structure-Guided Protein Engineering of Glyceraldehyde-3-phosphate Dehydrogenase from Corynebacterium glutamicum for Dual NAD/NADP Cofactor Specificity
Hyeoncheol Francis Son,
Hyeonjeong Yu,
Jiyeon Hong
et al.
Abstract:Since
the discovery of l-glutamate-producing Corynebacterium
glutamicum, it has evolved to be
an industrial workhorse. For biobased chemical production, suppling
sufficient amounts of the NADPH cofactor is crucial. Glyceraldehyde-3-phosphate
dehydrogenase (GAPDH), a glycolytic enzyme that converts glyceraldehyde-3-phosphate
(G3P) to 1,3-bisphosphoglycerate and produces NADH, is a major prospective
solution for the cofactor imbalance issue. In this study, we determined
the crystal structure of GAPDH from C. glu… Show more
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