Structure, gene cluster of the O antigen and biological activity of the lipopolysaccharide from the rhizospheric bacterium Ochrobactrum cytisi IPA7.2

Sigida, Elena N.; Kargapolova, K.Yu.; Shashkov, Alexander S.; Zdorovenko, Evelina L.; Ponomaryova, Тatiana S.; Meshcheryakova, A. A.; Tkachenko, Oksana V.; Burygin, Gennady L.; Knirel, Yuriy A.

doi:10.1016/j.ijbiomac.2019.11.017

Cited by 10 publications

(7 citation statements)

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“…and binding with amide bonds, as was previously described for the O. anthropi strain LMG3331 [43]. A difference in the fatty acid composition of lipid A of O. quorumnocens T1Kr02 is the absence of 3-hydroxyhexadecanoic and 3-hydroxyoctadecanoic acid residues, the presence of which was described for strains O. anthropi LMG3331 and O. intermedium LMG3301 [43] and O. cytisi IPA 7.2 [32]. The species Ochrobactrum quorumnocens together with the species O. rhizosphaerae, O. chromiisoli, O. pseudogrignonense, O. thiophenivorans, O. grignonense, and O. pituitosa within the Brucella/Ochrobactrum group form Clade 2 [44] or group Ochrobactrum_A (GTDB-Tk, https://github.com/kbaseapps/kb_gtdbtk accessed 16 January 2024) (Figure S3), phylogenetically remote from other Ochrobactrum spp.…”

Section: Discussionmentioning

confidence: 64%

“…For high-molecular-weight fractions of the O. rhizosphaerae strain PR17, the corresponding yields were 14% and 7% [34]. For phylogenetically more distant strains of Ochrobactrum spp., polysaccharide yields were significantly higher: 28% for O. anthropi LMG 3331 [45], 21% for O. cytisi IPA7.2 [32], 26.7% for O. endophyticum KCTC 42485 [26], 22% and 25% for two fractions of O. cytisi strain ESC1 [33].…”

Section: Discussionmentioning

confidence: 94%

“…Both characteristics of the particles are similar to those of the LPS of O. cytisi strain IPA7.2 (d = 66 nm; zetapotential = −22 mV; unpublished data). Stronger negative values of the zeta-potential of supramolecular particles of LPS of strains O. cytisi IPA7.2 and O. quorumnocens T1Kr02 relative to LPS of the strain O. anthropi LMG3331 (−11.9 mV) [43] are explained by the presence of negatively charged groups: D-ManNAcA residues for O. cytisi IPA7.2 [32] and succinate residues for O. quorumnocens T1Kr02. In this case, the diameter of LPS micelles from O. quorumnocens T1Kr02 was significantly larger than the diameter of LPS micelles from bacteria of the genus Azospirillum with similar zeta-potential values [54].…”

Section: Discussionmentioning

confidence: 97%

“…In this work, an LPS preparation was isolated from the dry biomass of O. quorumnocens T1Kr02 cells with a yield of 2.75%. For previously studied strains of Ochrobactrum spp., LPS yield was reported to be 8.7% for O. cytisi strain IPA 7.2 [32], 6.5% for O. endophyticum strain KCTC 42485 [26], and 2.35% for O. rhizosphaerae strain PR17 [34].…”

Section: Discussionmentioning

confidence: 99%

“…In addition to taxonomy, the study of LPS from bacteria of the genus Ochrobactrum has a number of other applied aspects. The LPS of these bacteria has been shown to influence the growth and development of plants [31,32]. It was also previously described that the O-polysaccharides of three Ochrobactrum strains contain residues of D-fucose (D-Fuc) [33,34] or its derivative (D-Fuc3NAcyl) [26].…”

Section: Introductionmentioning

confidence: 96%

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Structure, Physicochemical Properties and Biological Activity of Lipopolysaccharide from the Rhizospheric Bacterium Ochrobactrum quorumnocens T1Kr02, Containing d-Fucose Residues

Krivoruchko,

Zdorovenko,

Ivanova

et al. 2024

IJMS

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Lipopolysaccharides (LPSs) are major components of the outer membranes of Gram-negative bacteria. In this work, the structure of the O-polysaccharide of Ochrobactrum quorumnocens T1Kr02 was identified by nuclear magnetic resonance (NMR), and the physical–chemical properties and biological activity of LPS were also investigated. The NMR analysis showed that the O-polysaccharide has the following structure: →2)-β-d-Fucf-(1→3)-β-d-Fucp-(1→. The structure of the periplasmic glucan coextracted with LPS was established by NMR spectroscopy and chemical methods: →2)-β-d-Glcp-(1→. Non-stoichiometric modifications were identified in both polysaccharides: 50% of d-fucofuranose residues at position 3 were O-acetylated, and 15% of d-Glcp residues at position 6 were linked with succinate. This is the first report of a polysaccharide containing both d-fucopyranose and d-fucofuranose residues. The fatty acid analysis of the LPS showed the prevalence of 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, lactobacillic, and 27-hydroxyoctacosanoic acids. The dynamic light scattering demonstrated that LPS (in an aqueous solution) formed supramolecular particles with a size of 72.2 nm and a zeta-potential of –21.5 mV. The LPS solution (10 mkg/mL) promoted the growth of potato microplants under in vitro conditions. Thus, LPS of O. quorumnocens T1Kr02 can be recommended as a promoter for plants and as a source of biotechnological production of d-fucose.

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