Structure-Function Relationships of Micrococcus lysodeikticus Membranes: A Bacterial Membrane Model System

Saltón, M. R. J.

doi:10.1007/978-1-4615-7948-9_7

Cited by 10 publications

(4 citation statements)

References 182 publications

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“…cremoris HA cells by mutanolysininduced cell lysis (Rimpilainen et al 1986a) in the presence of 50 mmol/l Mgz+ ions (Munoz et al 1968). The membrane was washed twice with 0.5 mmol/l disodium EDTA in 50 mmol/l Tris-HCI buffer (pH 7.5) (Salton 1980) containing 1 mmol/l PMSF and F,-ATPase released with 3 mmol/l Tris-HCI buffer (pH 7.5) (Huberman & Salton 1979). Adenosine triphosphatase activity and protein of the fractions were analysed as previously (Rimpilainen et al 1986a).…”

Section: Methodsmentioning

confidence: 99%

Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F₁-ATPase preparations by polyacryacrylamide gel electrophoresis

Niskasaari

Juutinen

Rimpiläinen

et al. 1988

Lett Appl Microbiol

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The presence of lipoteichoic acid (LTA) in plasma membrane and released adenosine triphosphatase (ATPase) preparations of Streptococcus lactis subsp. cremoris HA was studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE). The membrane preparation gave two spots with periodic acid Schiff's (PAS) staining. The reference LTA revealed one CBB stainable band with MW 21 500, and was detected as a characteristic yellow spot in the molecular weight area 14 000–20 900 with silver staining and was also stainable with PAS. A slight colour was found with PAS in the F1‐ATPase (EC 3.6.1.3) preparation. The results suggest that the carbohydrate component in the membrane preparation and in the solubilized F1‐ATPase is LTA.

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Section: Methodsmentioning

confidence: 99%

Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F₁-ATPase preparations by polyacryacrylamide gel electrophoresis

Niskasaari

Juutinen

Rimpiläinen

et al. 1988

Lett Appl Microbiol

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“…In order to check whether the same kind of interactions could be detected with natural membranes, membranes isolated from Micrococcus luteus were added to an aqueous solution of ellipticine. This organism was chosen because all its phospholipids are acidic (see Materials and Methods and [14]). It is clear from Fig.2D, that at pH 7.12 (curve 2) ellipticine is in its protonated form and the curve shape is unchanged up to pH 8.1 (curve not shown).…”

Section: Results a N D Discussionmentioning

confidence: 99%

Interactions of Ellipticine with Model or Natural Membranes

Tercé¹,

Tocanne²,

Lanéelle³

1982

European Journal of Biochemistry

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Ellipticine is the type of a group of substances used against human cancer. It was shown that ellipticine and 9-methoxyellipticine strongly interact with monolayers of negatively charged phospholipids. In the present paper it is shown by spectrophotometric methods that ellipticine interacts with suspensions of a model membrane with a 131 charge neutralization, and with a natural membrane, both negatively charged. At a physiological pH, ellipticine undergoes protonation in the presence of these membranes. Its apparent pK is increased by 1, and its spectral behaviour, when the pH is changed, indicates that the drug is in a less polar environment, suggesting that hydrophobic bonds link the drug and the lipids. The existence of such bonds is suggested by the fact that the drug is not significantly released from anionic membranes when the pH is increased to 10, at which value ellipticine is not ionized. The consequences of these ionic and hydrophobic interactions are briefly discussed.

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“…The respiratory chain components of this organism have been studied in detail by Gel'man et al (1975) and Tikhonova (1974), and the F,-ATPase by Muiioz (1982) and Salton (1980). In addition, enzymes involved in the biosynthesis of cardiolipin and other membrane phospholipids, and the biosynthesis of lipomannan, peptidoglycan and teic huronic acid have been investigated in a number of laboratories (see review by Salton, 1980). The task of determining the topographical location of such enzymes would be a formidable undertaking.…”

Section: An Tigenic and Biochemical Architecture Of Micrococcus Lysodmentioning

confidence: 99%

The Sixteenth Marjory Stephenson Memorial Lecture

Saltón

1983

Microbiology

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It was with great joy that I received the invitation to present this year's Marjory Stephenson Memorial Lecture to pay tribute to one of the outstanding pioneers of microbial chemistry and I would like to thank the Society for this honour. For me it is a special privilege, as I was the last overseas student she accepted prior to her untimely death in the latter part of 1948. I have always regarded myself as being one of the very fortunate microbiologists to have known her and I have deeply regretted that it was not for a longer period of time. As an undergraduate in far-off Australia, her name was the hallmark for excellence in all matters microbiological and biochemical and Marjory Stephenson's Bacterial Metabolism was our 'bible'. It was cause for great excitement when I was awarded a CSIRO Studentship and accepted, as she wrote, 'for one of the two places reserved in our course for postgraduate students'. With bags and books packed, I embarked on the long journey from Sydney to Cambridge. In those pre-jet days the sea voyage to London took five weeks and it was that time of the year when many students and actors from Australia were heading off for the 'old country' to take up their scholarships and broaden their horizons. With such a mix of stage and science the voyage was rarely dull but we all eagerly awaited arrival and the experiences that lay ahead. The attractions and scientific exhilaration of Cambridge were so great that my stay became an extended one, thanks to Ernest Gale's hospitality as Director of the MRC Unit for Chemical Microbiology (later the Sub-Department of Chemical Microbiology, Department of Biochemistry) and his stimulus, enthusiasm and interest in the mysteries of the functioning of the bacterial surface. The brevity of my personal contact with Marjory Stephenson was fully compensated for by the enduring association with Ernest Gale and his colleagues in what was affectionately known as 'the bug hut'. Interest in the bacterial cell surface goes all the way back to the birth of microbiology with the microscopic observations of Antony van Leeuwenhoek recorded in his letters to the Royal Society in 1676. Leeuwenhoek not only described the principal shapes of bacteria but also noted that he was unable to see the surface 'film' which held them together, thereby anticipating the existence of structural components of the cell responsible for their characteristic shape. Much of the ensuing evidence on the existence and nature of a cell wall or membrane in bacteria was indirect, fragmentary and inconclusive. About the mid to late 1940s there was a renewed interest in the bacterial surface, stimulated by transport studies such as those of Gale & Taylor (1947) on the accumulation of amino acids across the permeability barrier of Gram-positive bacteria and the direct visualization of walls and membranes in disrupted and bacteriophage-lysed cells by electron microscopy (Mudd & Lackman, 1941 ; Mudd et al., 1942; Wyckoff, 1948). Indeed, the first Symposium held by this Society in 1949, 'The Nature of ...

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Structure-Function Relationships of Micrococcus lysodeikticus Membranes: A Bacterial Membrane Model System

Cited by 10 publications

References 182 publications

Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F₁-ATPase preparations by polyacryacrylamide gel electrophoresis

Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F₁-ATPase preparations by polyacryacrylamide gel electrophoresis

Interactions of Ellipticine with Model or Natural Membranes

The Sixteenth Marjory Stephenson Memorial Lecture

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Structure-Function Relationships of Micrococcus lysodeikticus Membranes: A Bacterial Membrane Model System

Cited by 10 publications

References 182 publications

Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F1-ATPase preparations by polyacryacrylamide gel electrophoresis

Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F1-ATPase preparations by polyacryacrylamide gel electrophoresis

Interactions of Ellipticine with Model or Natural Membranes

The Sixteenth Marjory Stephenson Memorial Lecture

Contact Info

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About

Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F₁-ATPase preparations by polyacryacrylamide gel electrophoresis

Release of adenosine triphosphatase from Streptococcus lactis subsp. cremoris membrane: evaluation of carbohydrate in membrane and F₁-ATPase preparations by polyacryacrylamide gel electrophoresis