Structure-function relationships of human meniscus

Danso, Elvis K.; Oinas, J.; Saarakkala, Simo; Mikkonen, Santtu; Töyräs, Juha; Korhonen, Rami K.

doi:10.1016/j.jmbbm.2016.12.002

Cited by 48 publications

(41 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to these differences in blood supply, the meniscus can be distinguished by the outer vascular region (red zone), inner avascular region (white zone) and the red-white zone between the red and white zones. The outer zone of the meniscus contains 90% type I collagen while the inner zone contains 60% type II collagen and 40% type I collagen 3…”

Section: Introductionmentioning

confidence: 99%

Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration

et al. 2019

View full text Add to dashboard Cite

ObjectivesThe heterogeneity of meniscus cells and the mechanism of meniscus degeneration is not well understood. Here, single-cell RNA sequencing (scRNA-seq) was used to identify various meniscus cell subsets and investigate the mechanism of meniscus degeneration.MethodsscRNA-seq was used to identify cell subsets and their gene signatures in healthy human and degenerated meniscus cells to determine their differentiation relationships and characterise the diversity within specific cell types. Colony-forming, multi-differentiation assays and a mice meniscus injury model were used to identify meniscus progenitor cells. We investigated the role of degenerated meniscus progenitor (DegP) cell clusters during meniscus degeneration using computational analysis and experimental verification.ResultsWe identified seven clusters in healthy human meniscus, including five empirically defined populations and two novel populations. Pseudotime analysis showed endothelial cells and fibrochondrocyte progenitors (FCP) existed at the pseudospace trajectory start. Melanoma cell adhesion molecule ((MCAM)/CD146) was highly expressed in two clusters. CD146+ meniscus cells differentiated into osteoblasts and adipocytes and formed colonies. We identified changes in the proportions of degenerated meniscus cell clusters and found a cluster specific to degenerative meniscus with progenitor cell characteristics. The reconstruction of four progenitor cell clusters indicated that FCP differentiation into DegP was an aberrant process. Interleukin 1β stimulation in healthy human meniscus cells increased CD318+ cells, while TGFβ1 attenuated the increase in CD318+ cells in degenerated meniscus cells.ConclusionsThe identification of meniscus progenitor cells provided new insights into cell-based meniscus tissue engineering, demonstrating a novel mechanism of meniscus degeneration, which contributes to the development of a novel therapeutic strategy.

show abstract

Section: Introductionmentioning

confidence: 99%

Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration

et al. 2019

View full text Add to dashboard Cite

show abstract

“…11,12 Although the structure of meniscus differs from that of articular cartilage, it has similar fibrous (collagen-based) protein content. Having established the relationship between meniscus biomechanical integrity and its collagen orientation/structure, 51 we recently demonstrated the potential of NIR spectroscopy for estimating human meniscus ECM composition, including collagen and PG contents determined biochemically. 33 This is due to the sensitivity of NIR spectroscopy to the physical, chemical, and morphological properties of soft tissues.…”

Section: Discussionmentioning

confidence: 99%

“…53 The modeled strain-dependent parameters, E ε and M k , which are related to the structural integrity of meniscus, are influenced by the PG and collagen contents and collagen orientation, and the effects are site-specific. 51 Thus, predicting these parameters could provide information on the functional integrity of meniscus as an indirect indicator of tissue health. While there are inherent variations in meniscus composition depending on location, 12,46 an earlier study has shown that the composition also changes with injury and other pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

Optical spectroscopic characterization of human meniscus biomechanical properties

et al. 2017

Self Cite

View full text Add to dashboard Cite

This study investigates the capacity of optical spectroscopy in the visible (VIS) and near-infrared (NIR) spectral ranges for estimating the biomechanical properties of human meniscus. Seventy-two samples obtained from the anterior, central, and posterior locations of the medial and lateral menisci of 12 human cadaver joints were used. The samples were subjected to mechanical indentation, then traditional biomechanical parameters (equilibrium and dynamic moduli) were calculated. In addition, strain-dependent fibril network modulus and permeability strain-dependency coefficient were determined via finite-element modeling. Subsequently, absorption spectra were acquired from each location in the VIS (400 to 750 nm) and NIR (750 to 1100 nm) spectral ranges. Partial least squares regression, combined with spectral preprocessing and transformation, was then used to investigate the relationship between the biomechanical properties and spectral response. The NIR spectral region was observed to be optimal for model development (83.0%≤R2≤90.8%). The percentage error of the models are: Eeq (7.1%), Edyn (9.6%), Eϵ (8.4%), and Mk (8.9%). Thus, we conclude that optical spectroscopy in the NIR range is a potential method for rapid and nondestructive evaluation of human meniscus functional integrity and health in real time during arthroscopic surgery.

show abstract

“…These functions allow for smoother movement and greater stability of the knee joint [2]. These functions can be attributed to the composition of the menisci, primarily type 1 collagen fiber bundles, which give them their characteristic tensile strength and stiffness [3]. For these reasons, meniscal injury can be a great impediment to the function of the knee.…”

Section: Introductionmentioning

confidence: 99%

Use of magnetic resonance imaging to determine laterality of meniscal size in healthy volunteers

Hamdan¹,

Haddad²,

Isleem

et al. 2020

PLoS ONE

View full text Add to dashboard Cite

Introduction The menisci are responsible for several functions. They are shock absorbers during dynamic loading on the knee and provide a broader surface area on which to distribute stress evenly to the tibia and femur. These functions allow for smoother movement and greater stability of the knee joint. Meniscal injury can be a great impediment to the function of the knee. Therefore, in the case of meniscal injury, our main concern is the relief of patient symptoms, followed by consequent restoration of meniscal function to the greatest of our ability. To prevent the long terms effects of a meniscectomy, meniscal allograft transplantation (MAT) was developed. The potential of using the size of the contralateral healthy menisci, to determine the size of the menisci to be replaced, will be discussed. Methods Knee MRIs done on healthy patients in the past 5 years were reviewed. Magnetic Resonance Imaging was performed using a 3-T scanner. Each individual was examined with knee joints in full extension. Measurements were performed two separate times, two weeks apart. A mean of three measurements was made during each session to reduce error. Thirty-eight normal bilateral knee joints MRIs remained (16 males, 22 females). Participants were sampled from the institutional Picture Archiving and Communication System (PACS). Age, gender, and the medial meniscal and lateral meniscal size of both knees were recorded. The laterality of the menisci was compared between both knees in each patient. Results A total of 38 patients were included in this study, with a mean age of 37.39 (±9.50) years. They were 16 (42.1%) men and 22 (57.9%) women. We didn't find any significant difference

show abstract

Structure-function relationships of human meniscus

Cited by 48 publications

References 36 publications

Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration

Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration

Optical spectroscopic characterization of human meniscus biomechanical properties

Use of magnetic resonance imaging to determine laterality of meniscal size in healthy volunteers

Contact Info

Product

Resources

About