Structure-Function Relationships in Muscle Fibres: <i>MyoRobot</i> Online Assessment of Muscle Fibre Elasticity and Sarcomere Length Distributions

Haug, Michael; Ritter, Paul; Michael, Mena; Reischl, Barbara; Schürmann, Sebastian; Prols, Gerhard; Friedrich, Oliver

doi:10.1109/tbme.2021.3089739

Cited by 3 publications

(2 citation statements)

References 38 publications

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“…Whether these potential interaction sites for DTT and H 2 O 2 translate to the effects seen in permeabilized muscle fibres and how they align with the proposed pathways in intact muscle fibres remains yet to be resolved. Further, it is unclear from the literature how and to what degree these ROS-mediated changes impact the axial elasticity of the fibre, as an important parameter for damage prevention, structural memory, and the ability to resist large strains [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot

Michael

Kovbasyuk

Ritter

et al. 2022

Cells

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An oxidizing redox state imposes unique effects on the contractile properties of muscle. Permeabilized fibres show reduced active force generation in the presence of H2O2. However, our knowledge about the muscle fibre’s elasticity or flexibility is limited due to shortcomings in assessing the passive stress–strain properties, mostly due to technically limited experimental setups. The MyoRobot is an automated biomechatronics platform that is well-capable of not only investigating calcium responsiveness of active contraction but also features precise stretch actuation to examine the passive stress–strain behaviour. Both were carried out in a consecutive recording sequence on the same fibre for 10 single fibres in total. We denote a significantly diminished maximum calcium-saturated force for fibres exposed to ≥500 µM H2O2, with no marked alteration of the pCa50 value. In contrast to active contraction (e.g., maximum isometric force activation), passive restoration stress (force per area) significantly increases for fibres exposed to an oxidizing environment, as they showed a non-linear stress–strain relationship. Our data support the idea that a highly oxidizing environment promotes non-linear fibre stiffening and confirms that our MyoRobot platform is a suitable tool for investigating redox-related changes in muscle biomechanics.

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Section: Introductionmentioning

confidence: 99%

Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot

Michael

Kovbasyuk

Ritter

et al. 2022

Cells

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“…These values are associated with a margin of error (roughly ±0.1 µm) arising from a Gaussian distribution of all SLs within a field-of-view around the median SL. Such Gaussian distributions of SLs are thoroughly reported in the literature [ 41 , 59 , 60 ] and extensively discussed in a previous publication [ 61 ]. Once 140% resting length was reached, maximum passive axial restoration force was analysed and normalized to the single-fibre cross-sectional area to yield restoration stress.…”

Section: Methodsmentioning

confidence: 87%

Levosimendan’s Effects on Length-Dependent Activation in Murine Fast-Twitch Skeletal Muscle

Haug,

Michael,

Ritter

et al. 2024

IJMS

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Levosimendan’s calcium sensitizing effects in heart muscle cells are well established; yet, its potential impact on skeletal muscle cells has not been evidently determined. Despite controversial results, levosimendan is still expected to interact with skeletal muscle through off-target sites (further than troponin C). Adding to this debate, we investigated levosimendan’s acute impact on fast-twitch skeletal muscle biomechanics in a length-dependent activation study by submersing single muscle fibres in a levosimendan-supplemented solution. We employed our MyoRobot technology to investigate the calcium sensitivity of skinned single muscle fibres alongside their stress–strain response in the presence or absence of levosimendan (100 µM). While control data are in agreement with the theory of length-dependent activation, levosimendan appears to shift the onset of the ‘descending limb’ of active force generation to longer sarcomere lengths without notably improving myofibrillar calcium sensitivity. Passive stretches in the presence of levosimendan yielded over twice the amount of enlarged restoration stress and Young’s modulus in comparison to control single fibres. Both effects have not been described before and may point towards potential off-target sites of levosimendan.

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Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells

Schneidereit

Nübler

Friedrich

2023

Methods in Molecular Biology

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Structure-Function Relationships in Muscle Fibres: MyoRobot Online Assessment of Muscle Fibre Elasticity and Sarcomere Length Distributions

Cited by 3 publications

References 38 publications

Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot

Redox Balance Differentially Affects Biomechanics in Permeabilized Single Muscle Fibres—Active and Passive Force Assessments with the Myorobot

Levosimendan’s Effects on Length-Dependent Activation in Murine Fast-Twitch Skeletal Muscle

Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells

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