Structure, function, and defect tolerance with maturation of the radial tie fiber network in the knee meniscus

Bansal, Sonia; Peloquin, John M.; Keah, Niobra M; O'Reilly, Olivia C.; Elliott, Dawn M.; Mauck, Robert L.; Zgonis, Miltiadis H.

doi:10.1002/jor.24697

Cited by 20 publications

(17 citation statements)

References 33 publications

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“…Meniscus cells also proliferated to a greater extent on dMEP scaffolds and showed higher viability compared to the synthetic PCL- This study established the potential of dME inclusion to create biohybrid scaffolds which work to promote meniscus cell phenotype over long term culture and provides a foundation for further refinement and translation. One limitation of the scaffold is its physical properties: it is a sheetbased scaffold with an elastic modulus that is ~3.5% of the elastic modulus of the radial region of the juvenile meniscus, and the ultimate strength of the scaffold is ~20% of native [63]. Therefore, the dMEP scaffold would be most useful in situations where structural support is less necessary or after tissue deposition and scaffold maturation has occurred in vitro prior to implantation [64].…”

Section: Discussionmentioning

confidence: 99%

Development of A Decellularized Meniscus Matrix-Based Nanofibrous Scaffold for Meniscus Tissue Engineering

Xia

Kim

Bansal

et al. 2020

Preprint

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The meniscus plays a critical role in knee mechanical function but is commonly injured given its central load bearing role. In the adult, meniscus repair is limited, given the low number of endogenous cells, the density of the matrix, and the limited vascularity. Menisci are fibrocartilaginous tissues composed of a micro-/nano-fibrous extracellular matrix (ECM) and a mixture of chondrocyte-like and fibroblast-like cells. Here, we developed a fibrous scaffold system that consists of bioactive components (decellularized meniscus ECM (dME) within a poly(e-caprolactone) material) fashioned into a biomimetic morphology (via electrospinning) to support and enhance meniscus cell function and matrix production. This work supports that the incorporation of dME into synthetic nanofibers increased hydrophilicity of the scaffold, leading to enhanced meniscus cell spreading, proliferation, and fibrochondrogenic gene expression. This work identifies a new biomimetic scaffold for therapeutic strategies to substitute or replace injured meniscus tissue.STATEMENT OF SIGNIFICANCEIn this study, we show that a scaffold electrospun from a combination of synthetic materials and bovine decellularized meniscus ECM provides appropriate signals and a suitable template for meniscus fibrochondrocyte spreading, proliferation, and secretion of collagen and proteoglycans. Material characterization and in vitro cell studies support that this new bioactive material is susceptible to enzymatic digestion and supports meniscus-like tissue formation.

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Section: Discussionmentioning

confidence: 99%

Development of A Decellularized Meniscus Matrix-Based Nanofibrous Scaffold for Meniscus Tissue Engineering

Xia

Kim

Bansal

et al. 2020

Preprint

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“…Medial menisci were prepared for further analysis as in previous work. 7 Briefly, anterior and posterior horns were separated from the meniscal body. These were radially sectioned to yield a wedge-shaped cross section using a cryostat (model HM500; Microm International GmbH).…”

Section: Methodsmentioning

confidence: 99%

Six-Month Outcomes of Clinically Relevant Meniscal Injury in a Large-Animal Model

Bansal

Meadows

Miller

et al. 2021

Orthopaedic Journal of Sports Medicine

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Background: The corrective procedures for meniscal injury are dependent on tear type, severity, and location. Vertical longitudinal tears are common in young and active individuals, but their natural progression and impact on osteoarthritis (OA) development are not known. Root tears are challenging and they often indicate poor outcomes, although the timing and mechanisms of initiation of joint dysfunction are poorly understood, particularly in large-animal and human models. Purpose/Hypothesis: In this study, vertical longitudinal and root tears were made in a large-animal model to determine the progression of joint-wide dysfunction. We hypothesized that OA onset and progression would depend on the extent of injury-based load disruption in the tissue, such that root tears would cause earlier and more severe changes to the joint. Study Design: Controlled laboratory study. Methods: Sham surgeries and procedures to create either vertical longitudinal or root tears were performed in juvenile Yucatan mini pigs through randomized and bilateral arthroscopic procedures. Animals were sacrificed at 1, 3, or 6 months after injury and assessed at the joint and tissue level for evidence of OA. Functional measures of joint load transfer, cartilage indentation mechanics, and meniscal tensile properties were performed, as well as histological evaluation of the cartilage, meniscus, and synovium. Results: Outcomes suggested a progressive and sustained degeneration of the knee joint and meniscus after root tear, as evidenced by histological analysis of the cartilage and meniscus. This occurred in spite of spontaneous reattachment of the root, suggesting that this reattachment did not fully restore the function of the native attachment. In contrast, the vertical longitudinal tear did not cause significant changes to the joint, with only mild differences compared with sham surgery at the 6-month time point. Conclusion: Given that the root tear, which severs circumferential connectivity and load transfer, caused more intense OA compared with the circumferentially stable vertical longitudinal tear, our findings suggest that without timely and mechanically competent fixation, root tears may cause irreversible joint damage. Clinical Relevance: More generally, this new model can serve as a test bed for experimental surgical, scaffold-based, and small molecule–driven interventions after injury to prevent OA progression.

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“…The most common lesions site is the posterior horn or body of the medial meniscus with the highest percentage of the overall load, especially when the knee is exed and the backward shear force is applied [4,5] . Biomechanical studies showed that the medial meniscus plays an important role in the transfer of shear force and longitudinal loads [11] . The percentage of total load carried by the posterior horn of the medial meniscus is the highest, especially in knee exion and backward shear force [12] .…”

Section: Resultsmentioning

confidence: 99%

Varus and Steep Medial Posterior Tibial Slope Are Risk Factors for Degenerative Medial Meniscus Lesions

et al. 2021

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Purpose: Degenerative medial meniscus lesions(DMMLs) is different from other meniscus injuries, which have a high incidence and easy to miss diagnosis in the middle-aged and elderly. The present study was designed to identify the risk factors for DMMLs among an Asian sample.Methods: The experimental group included 121 patients（ones partly confirmed during arthroscopic surgery) with DMMLs and the control group included 51 patients with no pathological changes identified by using 3.0-T magnetic resonance imaging (MRI) from January 2017 to January 2021 were analyzed retrospectively. By full-length anteroposterior radiographs of lower limbs in weight-bearing position of the two groups, the Hip-Knee-Ankle (HKA) angle in the coronal plane and the Medial Posterior Tibial Slope(MPTS) in the sagittal plane were measured by the MRI T1 sequence of the knee. The potential risk factors of DMMLs were analyzed by multivariate logistic regression. The independent variables included gender, age, body mass index (BMI), occupational kneeling, Kellgren-Lawrence (K-L) grade, HKA, and MPTS.Results: T-test analysis between the Experimental Group and the Control Group showed statistically significant differences in age (t=10.718, p<0.001), BMI (t=7.300, p<0.001), HKA (t=8.677, p<0.001), and MPTS (t=5.025, p<0.001). Chi-square test analysis between the two groups showed no statistically significant differences in gender (t=0.183, p=0.669) and occupational kneeling (t=0.339, p=0.560). Non-parametric analysis showed statistically significant differences in K-L (z=5.857, p<0.001) between the two groups. Logistic regression analysis showed that age, BMI, HKA, and MPTS were risk factors for DMMLs among the above-mentioned variables with statistically significant differences.Conclusions: varus, steep MPTS, advancing age and obesity were risk factors for DMMLs.

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Structure, function, and defect tolerance with maturation of the radial tie fiber network in the knee meniscus

Cited by 20 publications

References 33 publications

Development of A Decellularized Meniscus Matrix-Based Nanofibrous Scaffold for Meniscus Tissue Engineering

Development of A Decellularized Meniscus Matrix-Based Nanofibrous Scaffold for Meniscus Tissue Engineering

Six-Month Outcomes of Clinically Relevant Meniscal Injury in a Large-Animal Model

Varus and Steep Medial Posterior Tibial Slope Are Risk Factors for Degenerative Medial Meniscus Lesions

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