Structure elucidation and in vitro biological evaluation of sulfated exopolysaccharide from LAB Weissella paramesenteroides MN2C2

Amer, Mai N.; El-Gammal, Eman W.; Atwa, Nagwa A; El‐Diwany, Ahmed I.; Dawoud, Insaf E.; Rashad, Ferial M.

doi:10.7324/japs.2021.110504

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…It is one of the major causes of bacterial diseases in fish and there is growing evidence of P. aeruginosa in foodborne infections (Bricha et al, 2009;Nawaz and Bhattarai, 2015;Virupakshaiah and Hemalata, 2016). Various Pseudomonas species are multidrug resistant and resistance is likely to evolve over time, which explains why the number of effective antibiotics is decreasing and this may pose threat to public health (Algammal et al, 2020;Ayman et al, 2022). It is therefore important to determine the role of O. niloticus fish as a reservoir of P. aeruginosa.…”

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confidence: 99%

Prevalence and antimicrobial resistance profile of bacterial foodborne pathogens in Nile tilapia fish (Oreochromis niloticus) at points of retail sale in Nairobi, Kenya

Mumbo,

Nyaboga,

Kinyua

et al. 2023

Front. Antibiot.

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Proteus spp., Staphylococcus spp., Pseudeomonas spp., and pathogenic Vibrios are among the major foodborne pathogens associated with the consumption of contaminated fish. The increasing occurrence of antimicrobial resistance in these pathogens is a serious public health concern globally and therefore continuous monitoring of antimicrobial resistance of these bacteria along the food chain is crucial for for control of foodborne illnesses. The aim of this study was to assess the prevalence, antimicrobial resistance patterns, antibiotic resistance genes, and genetic diversity of bacterial foodborne pathogens recovered from fresh Nile tilapia (Oreochromis niloticus) obtained from retail markets in Nairobi, Kenya. A total of 68 O. niloticus fish with an average weight of 300.12 ± 25.66 g and body length of 23.00 ± 0.82 cm were randomly sampled from retail markets and tested for the presence of Proteus, Staphylococcus aureus, Pseudomonas aeruginosa, Vibrio cholerae, and Vibrio parahaemolyticus. Standard culture-based microbiological and Kirby–Bauer agar disk diffusion methods were used to isolate and determine the antimicrobial resistance patterns of the isolates to 11 selected antibiotics. Statistical analysis was performed using Minitab v17.1, with p < 0.05 considered significant. The genetic diversity of the multidrug-resistant (MDR) and extensively drug-resistant (XDR) bacteria was determined using 16S rRNA sequencing and phylogenetic analysis, and polymerase chain reaction (PCR) was used for detection of antibiotic resistance genes in MDR bacterial isolates. High levels of bacterial contamination were detected in fresh O. niloticus fish (44/68, 64.71%). The most prevalent bacteria were Proteus spp. (44.12%), with the rest of the bacterial species registering a prevalence of 10.29%, 4.41%, 2.94%, and 2.94% (for S. aureus, P. aeruginosa, V. cholerae, and V. parahaemolyticus, respectively). Antimicrobial resistance was detected in all the bacteria species and all the isolates were resistant to at least one antibiotic except cefepime (30 µg). Additionally, 86.36% of the isolates exhibited multidrug resistance, with higher multiple antibiotic resistance indices (MAR index >0.3) indicating that fresh O. niloticus fish were highly contaminated with MDR bacteria. Results of 16S rRNA sequences, BLASTn analysis, and phylogenetic trees confirmed the identified MDR bacterial isolates as Proteus mirabilis and other Proteus spp., S. aureus, P. aeruginosa, V. cholerae, and V. parahaemolyticus. PCR analysis confirmed the presence of multiple antibiotic resistance genes blaTEM-1, blaCMY-2, tetA, tetC, Sul2, dfrA7, strA, and aadA belonging to β-lactamases, tetracycline, sulfonamide, trimethoprim, and aminoglycosides in all the MDR bacterial isolates. There was strong correlation between antibiotic- resistant genes and phenotypic resistance to antibiotics of MDR bacteria. This study showed high prevalence of multidrug resistance among foodborne bacterial isolates from fresh O. niloticus fish obtained from retail markets. From this study, we conclude that fresh O. niloticus fish are a potential source of MDR bacteria, which could be a major risk to public health as a consequence of their dissemination along the human food chain. These results highlight the prevalence of antimicrobial-resistant foodborne pathogens in fish purchased from retail markets and underscore the risk associated with improper handling of fish.

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Section: Introductionmentioning

confidence: 99%

Prevalence and antimicrobial resistance profile of bacterial foodborne pathogens in Nile tilapia fish (Oreochromis niloticus) at points of retail sale in Nairobi, Kenya

Mumbo,

Nyaboga,

Kinyua

et al. 2023

Front. Antibiot.

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Synthesis and characterization of faujasite zeolite membrane for selective enrichment of Arthrobacter sp. in synthetic wastewater

El-kordy,

Kanzy,

Elgamouz

et al. 2024

Water Science &Amp; Technology

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This paper centers on the preparation and characterization of both a clay support and a faujasite zeolite membrane. Additionally, the study explores the development of bacterial media to assess the performance of these prepared membranes. The faujasite zeolite membrane was created using the hydrothermal method, involving the deposition of a faujasite layer to fine-tune the pore sizes of the clay support. The clay supports were crafted from a clay was sieved to particle size Φ ≤ 63 μm, and compacted with 3.0 wt.% activated carbon, then sintered at 1,000 °C. Distilled water fluxes revealed a decrease from 1,500 L m−2 h−1 to a minimum of 412 L m−2 h−1 after 180 min of filtration. Both membranes were characterized by XRF, XRD, FTIR, adsorption–desorption of nitrogen (N2), and SEM-EDS. PCR technique was used for the identification of the isolated Arthrobacter sp., and the retention of the bacteria on the clay support and the faujasite zeolite membrane were found to be 96 and 99%, respectively. The results showed that the faujasite zeolite membrane passed the clay support due to a narrow of the faujasite zeolite membrane of 2.28 nm compared to 3.55 nm for the clay supports.

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Molecular Characterization and Biocompatibility of Exopolysaccharide Produced by Moderately Halophilic Bacterium Virgibacillus dokdonensis from the Saltern of Kumta Coast

Andrew

Jayaraman

2022

Polymers

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The use of natural polysaccharides as biomaterials is gaining importance in tissue engineering due to their inherent biocompatibility. In this direction, the present study aims to explore the structure and biocompatibility of the EPS produced by Virgibacillus dokdonensis VITP14. This marine bacterium produces 17.3 g/L of EPS at 96 h of fermentation. The EPS was purified using ion exchange and gel permeation chromatographic methods. The porous web-like structure and elemental composition (C, O, Na, Mg, P, S) of the EPS were inferred from SEM and EDX analysis. AFM analysis revealed spike-like lumps with a surface roughness of 84.85 nm. The zeta potential value of −10 mV indicates the anionic nature of the EPS. Initial molecular characterization showed that the EPS is a heteropolysaccharide composed of glucose (25.8%), ribose (18.6%), fructose (31.5%), and xylose (24%), which are the monosaccharide units in the HPLC analysis. The FTIR spectrum indicates the presence of functional groups/bonds typical of EPSs (O-H, C-H, C-O-H, C-O, S=O, and P=O). The polymer has an average molecular weight of 555 kDa. Further, NMR analysis revealed the monomer composition, the existence of two α- and six β-glycosidic linkages, and the branched repeating unit as → 1)[α-D-Xylp-(1 → 2)-α-D-Glcp-(1 → 6)-β-D-Glcp-(1 → 5)]-β-D-Frup-(2 → 2)[β-D-Xylp-(1 → 4)]-β-D-Xylp-(1 → 6)-β-D-Fruf-(2 → 4)-β-D-Ribp-(1 →. The EPS is thermally stable till 251.4 °C. X-ray diffraction analysis confirmed the semicrystalline (54.2%) nature of the EPS. Further, the EPS exhibits significant water solubility (76.5%), water-holding capacity (266.8%), emulsifying index (66.8%), hemocompatibility (erythrocyte protection > 87%), and cytocompatibility (cell viability > 80% on RAW264.7 and keratinocyte HaCaT cells) at higher concentrations and prolongs coagulation time in APTT and PT tests. Our research unveils the significant biocompatibility of VITP14 EPS for synthesizing a variety of biomaterials.

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Structure elucidation and in vitro biological evaluation of sulfated exopolysaccharide from LAB Weissella paramesenteroides MN2C2

Cited by 3 publications

References 31 publications

Prevalence and antimicrobial resistance profile of bacterial foodborne pathogens in Nile tilapia fish (Oreochromis niloticus) at points of retail sale in Nairobi, Kenya

Prevalence and antimicrobial resistance profile of bacterial foodborne pathogens in Nile tilapia fish (Oreochromis niloticus) at points of retail sale in Nairobi, Kenya

Synthesis and characterization of faujasite zeolite membrane for selective enrichment of Arthrobacter sp. in synthetic wastewater

Molecular Characterization and Biocompatibility of Exopolysaccharide Produced by Moderately Halophilic Bacterium Virgibacillus dokdonensis from the Saltern of Kumta Coast

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