Structure, dynamics, and energetics of deoxyguanosine-thymidine wobble base pair formation in the self-complementary d(CGTGAATTCGCG) duplex in solution

Hairpin and duplex formation of the DNA octamer d(m5C-G-m5C-G-T-G-m5C-G) in solution. An NMR study

“…For example, it is known from X-ray crystallography of single crystals (3,4) that the G.T mismatch in A, B and Z helices forms a hydrogen-bonded pair with the bases as major tautomers and in the anti orientation around the glycosyl bond. Similar results were obtained by Patel et al (5), who investigated the NMR spectra of a B-DNA dodecamer containing the G.T mismatch. The vertical base stacking of the G.T wobble bases upon their nearest neighbours appears to be affected to some extent.…”

Comparison of the conformation of an oligonucleotide containing a central G-T base pair with the non-mismatch sequence by proton NMR

“…But only two have been observed here, as elsewhere (21)(22)(23). A number of possibilities have been considered to account for this: for a rare tautomer we could observe tither (a) the hydroxyl and imino proton with the 6 amino protons being very broad via same exchange mechanism or (b) we could see the 6 mino protons with the others exchange broadened, while for the wobble structure we could observe either (c) the two iminos protons or (d) only the 6 amino protons.…”

Elementary Molecular Mechanisms of the Spontaneous Point Mutations in DNA: A Novel Quantum-Chemical Insight into the Classical Understanding

“…It is also generally accepted in the literature that wobble base pairs (Gua·Thy and Ade·Cyt) (Brown et al, 1985;Crick, 1966;Hunter et al, 1986;Kennard, 1985;Padermshoke et al, 2008;Patel et al, 1982aPatel et al, , 1982bPatel et al, , 1984aPatel et al, , 1984b formed by bases in their canonical tautomeric forms www.intechopen.com…”

“…The wobble mispairings were observed in X-ray (Brown et al, 1985;Hunter et al, 1986;Kennard, 1985) and NMR (Patel et al, 1982a(Patel et al, , 1982b(Patel et al, , 1984a(Patel et al, , 1984b model experiments (in the absence of DNA polymerases) on cocrystallization of complementary oligonucleotides containing a single mismatched base pair. But such experimental conditions do not properly reflect those required for enzymatic DNA replication (Kornberg & Baker, 1992).…”