Structure determination of inonotsuoxides A and B and in vivo anti-tumor promoting activity of inotodiol from the sclerotia of Inonotus obliquus

Progress on Understanding the Anticancer Mechanisms of Medicinal Mushroom: Inonotus Obliquus

“…Li et al (2003) reported that I. obliquus extracts with concentration of 0.5 μg•mL-1~16.0 μg•mL-1 can inhibit the proliferation and induce apoptosis of the gastric cancer cell line MGC-803, the effect of which displays a dose-response. The most abundant triterpene, inotodiol (3), was investigated by Nakata et al (2007) for the inhibitory effect in a two-stage carcinogenesis test on mouse skin using 7,12-dimethylbenz[a]anthracene (DMBA) as an initiator and TPA as a promoter. Compound 3 was found to exhibit the potent anti-tumor promoting activity in the in vivo carcinogenesis test.…”

Identification of a novel blocker of IκBα kinase activation that enhances apoptosis and inhibits proliferation and invasion by suppressing nuclear factor-κB

“…Because Chaga and DBL derived from it reportedly exhibit growth-inhibitory effects (11,15), we postulated that these effects may be mediated through modulation of the NF-nB activation pathway. To test this hypothesis, we investigated the effect of the DBL on NF-nB activation induced by several inflammatory agents in various cell types, including those in which NF-nB is constitutively active.…”

Inotodiol Inhabits Proliferation and Induces Apoptosis through Modulating Expression of cyclinE, p27, bcl-2, and bax in Human Cervical Cancer HeLa Cells

“…Inotodiol, a lanostane triterpenoids, from a chloroform extract of I. obliquus sclerotia (Nakata et al, 2007), exhibited significant anti-proliferative effects against mouse leukemia P388 cells (Nomura et al, 2008), Walker 256 carcinosarcoma cells and human mammary adenocarcinoma MCF-7 cells (KahlosK, 1987). In this study, inhibitory rate of HeLa cells was detected by MTT assay after treated with different concentrations of Inotodiol for 24h and 48h.…”

“…Inonotus obliquus (I. obliquus) is a medicinal mushroom that has been used as an effective agent to treat various diseases such as RESEARCH ARTICLE 1 1 1 1 2 * diabetes, tuberculosis and cancer (Zhong et al, 2009;Song et al, 2013). Inotodiol, a lanostane triterpenoid extract of I. obliquus biological effects, including anti-tumor (KahlosK, 1987;Nakata et al, 2007), anti-oxidant (Zhao et al, 2012), anti mutation (Ham et al, 2009). Recent reports showed that Inotodiol could inhibit proliferation and induce the apoptosis of A549 cells in vitro (Zhong et al, 2011), moreover, Inotodiol have low cytotoxicity against normal cells (Mi et al, 2010).…”