Structure, Chromosome Location, and Expression of the Mouse Zinc Finger Gene <i>Krox-20</i>: Multiple Gene Products and Coregulation with the Proto-Oncogene c-<i>fos</i>

Chavrier, Philippe; Janssen-Timmen, U.; Mattei, Marie-Genevı̀eve; Bravo, Rodrigo; Charnay, Patrick

doi:10.1128/mcb.9.2.787-797.1989

Cited by 10 publications

(1 citation statement)

References 72 publications

(75 reference statements)

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“…These genes are members of the same families as those for the relatively ubiquitous proteins c-Fos (11,64) and Krox-24-egr-1 (35,36,60), but they are not induced in either regenerating liver or insulin-stimulated H-35 cells. Genes in both groups, ubiquitous and tissue specific, contain serum response elements within their promoters (8,18,63). This element has been implicated in the control of both induction and repression of immediate-early gene expression (18,63).…”

Section: Resultsmentioning

confidence: 99%

The Immediate-Early Growth Response in Regenerating Liver and Insulin-Stimulated H-35 Cells: Comparison with Serum-Stimulated 3T3 Cells and Identification of 41 Novel Immediate-Early Genes

Mohn

Laz

Hsu

et al. 1991

Molecular and Cellular Biology

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Liver regeneration provides a unique system for analysis of mitogenesis in intact, fully developed animals. Cellular immediate-early genes likely play an important role in cell cycle regulation and have been extensively studied in mitogen-stimulated fibroblasts lymphocytes but not in liver. We have begun to characterize the immediate-early growth response genes of mitogen-stimulated liver cells, specifically, regenerating liver and insulin-stimulated Reuber H-35 hepatoma cells, and to address differences in growth response between different cell types. Through subtraction and differential screening of cDNA libraries from regenerating liver and insulin-treated H-35 cells, we have extensively characterized 341 differentially expressed clones and identified 52 immediate-early genes. These genes have been partially sequenced and subjected to Northern (RNA) blot analysis, and 41 appear to be novel. Surprisingly, two-thirds of these genes are also expressed in BALB/c 3T3 cells, but only 10 were identified in previous studies of 3T3 cells, and of these, 6 include well-known genes like jun and fos, and only 4 are novel. Approximately one-third of the immediate-early genes identified in mitogen-stimulated liver cells or serum-stimulated NIH 3T3 cells are expressed in a tissue-specific fashion, indicating that cell type-specific regulation of the proliferative response occurs during the immediate-early period. Our findings indicate that the immediate-early response is unusually complex for the first step in a regulatory cascade, suggesting that multiple pathways must be activated. The abundance of immediate-early genes and the highly varied pattern of their expression in different cell types suggest that the tissue specificity of the proliferative response arises from the particular set of these genes expressed in a given tissue.

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Section: Resultsmentioning

confidence: 99%

The Immediate-Early Growth Response in Regenerating Liver and Insulin-Stimulated H-35 Cells: Comparison with Serum-Stimulated 3T3 Cells and Identification of 41 Novel Immediate-Early Genes

Mohn

Laz

Hsu

et al. 1991

Molecular and Cellular Biology

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Deletion of TECRL promotes skeletal muscle repair by up-regulating EGR2

Geng,

Liu,

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Myogenic regeneration relies on the proliferation and differentiation of satellite cells. TECRL (trans-2,3-enoyl-CoA reductase like) is an endoplasmic reticulum protein only expressed in cardiac and skeletal muscle. However, its role in myogenesis remains unknown. We show that TECRL expression is increased in response to injury. Satellite cell-specific deletion of TECRL enhances muscle repair by increasing the expression of EGR2 through the activation of the ERK1/2 signaling pathway, which in turn promotes the expression of PAX7. We further show that TECRL deletion led to the upregulation of the histone acetyltransferase general control nonderepressible 5, which enhances the transcription of EGR2 through acetylation. Importantly, we showed that AAV9-mediated TECRL silencing improved muscle repair in mice. These findings shed light on myogenic regeneration and muscle repair.

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Presence and phosphorylation of transcription factors in developing dendrites

Crino

Khodakhah

Becker

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

140

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In screening amplified poly(A) mRNA from hippocampal dendrites and growth cones in culture to determine candidates for local translation, we found that select transcription factor mRNAs were present. We hypothesized that synthesis of transcription factor proteins within dendrites would provide a direct signaling pathway between the distal dendrite and the nucleus resulting in modulation of gene expression important for neuronal differentiation. To evaluate this possibility, radiolabeled amplified antisense RNA was used to probe slot blots of transcription factor cDNAs as well as arrayed blots of zinc finger transcription factors. The mRNAs encoding the cAMP response element binding protein (CREB), zif 268, and one putative transcription factor were detected. We expanded upon these results showing that CREB protein is present in dendrites, that translation of CREB mRNA in isolated dendrites is feasible and that CREB protein found in dendrites can interact with the cis-acting cyclic AMP reponse element DNA sequence by using an in situ Southwestern assay. Further, CREB protein in dendrites is not transported to this site from the cell body because fluorescently tagged CREB microperfused into the soma did not diffuse into the dendrites. In addition, CREB protein microperfused into dendrites was rapidly transported to the nucleus, its likely site of bioactivity. Lastly, by using the isolated dendrite system we show that phosphorylation of Ser-133 on CREB protein can occur in isolated dendrites independent of the nucleus. These data provide a regulatory pathway in which transcription factors synthesized and posttranslationally modified in dendrites directly alter gene expression bypassing the integration of signal transduction pathways that converge on the nucleus.

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Structure, Chromosome Location, and Expression of the Mouse Zinc Finger Gene Krox-20: Multiple Gene Products and Coregulation with the Proto-Oncogene c-fos

Cited by 10 publications

References 72 publications

The Immediate-Early Growth Response in Regenerating Liver and Insulin-Stimulated H-35 Cells: Comparison with Serum-Stimulated 3T3 Cells and Identification of 41 Novel Immediate-Early Genes

The Immediate-Early Growth Response in Regenerating Liver and Insulin-Stimulated H-35 Cells: Comparison with Serum-Stimulated 3T3 Cells and Identification of 41 Novel Immediate-Early Genes

Deletion of TECRL promotes skeletal muscle repair by up-regulating EGR2

Presence and phosphorylation of transcription factors in developing dendrites

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