Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from <i>Dendrobium officinale</i>

Wei, Wei; Feng, Lei; Bao, Wan-Rong; Ma, Dik‐Lung; Leung, Chung‐Hang; Nie, Shaoping; Han, Quan‐Bin

doi:10.1021/acs.jafc.5b05180

Cited by 134 publications

(41 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Activation of macrophages can enhance their antitumor activity through secretion of cytokines or regulation of the immune system [ 29 ]; thereby, it is commonly assessed to examine the immunomodulatory function of polysaccharides [ 21 , 30 ]. In this study, macrophage immunological activity assay revealed that RS-3-1, BZ-3-1, FL-3-1, and GC-3-1 could enhance phagocytosis, promote NO production, and increase tumor necrosis factor (TNF- α ) level in RAW 264.7 cells.…”

Section: Discussionmentioning

confidence: 99%

“…RAW264.7 cells (1 × 10 4 cells/well) were seeded in 24-well plates and incubated overnight. Serial dilutions of polysaccharide samples (1, 10, 50, 100, and 500 μ g/mL), DMEM medium and LPS (5 μ g/mL) were then added, and the cells were incubated for another 24 h. The supernatants were collected and analyzed using an ELISA kit according to the manufacturer's instructions to determine the level of TNF- α secretion [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Purification, Preliminary Characterization, and Immunological Activity of Polysaccharides from Crude Drugs of Sijunzi Formula

Wang

Peng

et al. 2017

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Sijunzi Decoction (SJZD) is a conventional prescription for curing spleen deficiency in Traditional Chinese Medicine and polysaccharide is its main ingredient. In order to explore the effective ingredients contributing to the immunological activity of SJZD, we isolated and purified seven homogeneous polysaccharides from Radix Ginseng (RS-3-1 and RS-3-2), Rhizoma Atractylodis Macrocephalae (BZ-3-1, BZ-3-2, and BZ-3-3), Poria (FL-3-1), and Radix Glycyrrhizae (GC-3-1) decoctions, respectively. The molecular weight of seven homogeneous polysaccharides ranged from 5.42 × 104 to 5.65 × 104 Da. Monosaccharide composition determined by GC-MS analysis showed that these polysaccharides were primarily composed of Rha, Ara, Xyl, Man, Glc, and Gal with various ratios. Immunological activity assay revealed that polysaccharides from four crude drug components of SJZD displayed inhibitory effects on the complement system. RS-3-1, BZ-3-1, FL-3-1, and GC-3-1 could significantly enhance the phagocytosis and increase the NO production and tumor necrosis factor (TNF-α) level in RAW 264.7 cells (p < 0.05). These results demonstrated the immunological activities of these polysaccharides from the four crude drugs. This study supports the therapeutic effect of SJZD in clinical use and is essential for further identification the immunopolysaccharide from SJZD decoction.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Purification, Preliminary Characterization, and Immunological Activity of Polysaccharides from Crude Drugs of Sijunzi Formula

Wang

Peng

et al. 2017

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…CellTiter 96 ® AQueous One Solution Cell Proliferation kit was purchased from Promega Inc. (Madison, WI, USA). Polysaccharide marker DOP of Dendrobium officinale was prepared in our previous study [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…In our previous studies, a unique polysaccharide marker (glucomannan, called DOP) was identified for the authentication of D. officinale [ 13 ]. DOP is likely also to be the main active ingredient because its content exceeds 30% of the dry herb by weight, and it shows immunomodulating effects towards immune cells [ 14 , 15 , 16 , 17 ]. Therefore, we hypothesize that DOP also has anti-fatigue activity.…”

Section: Introductionmentioning

confidence: 99%

Anti-Fatigue Effects of the Unique Polysaccharide Marker of Dendrobium officinale on BALB/c Mice

Wei

Zhu

et al. 2017

Molecules

Self Cite

View full text Add to dashboard Cite

Dendrobium officinale extract shows potent anti-fatigue effects; however, the active substance responsible for these effects remains undetermined. A glucomannan with a huge molecular size of 730 kDa, called DOP, was identified as the unique authentication marker of this expensive herb. DOP exhibited immunomodulating effects on macrophages and lymphocytes in our previous study. Clinical reports also showed that people with fatigue syndrome have a disturbed immune system. Because DOP is the unique and dominant component of D. officinale, we hypothesize that DOP may also have anti-fatigue activity. The present study aims to evaluate the anti-fatigue activity of DOP on BALB/c mice, with Rhodiola rosea extract as a positive control. DOP and Rhodiola rosea extract were orally administered at doses of 50 mg/kg and 100 mg/kg, respectively, for four weeks, and the anti-fatigue activity of DOP on BALB/c mice was evaluated using the weight-loaded swimming test. The contents of lactic dehydrogenase (LDH), creatine phosphokinase (CK), triglyceride (TG), blood urea nitrogen (BUN), superoxide dismutase (SOD), malondialdehyde (MDA), lactic acid (LD), and glutathione peroxidase (GSH-Px) in serum, glycogen of liver and gastrocnemius muscle were also determined. Their effects on variability of T cells and B cells were determined by using tetrazolium compound (MTS) method. The weight-loaded swimming exercise caused fatigue syndrome, mainly including the decreases of serum SOD/GSH-Px and gastrocnemius glycogen, as well as the increases of LDH, BUN, MDA, CK, TG, and LD in serum. All of these indicators of fatigue were inhibited to a certain extent by both DOP and Rhodiola rosea extract; however, the effects of DOP were much stronger than those of Rhodiola rosea extract. Compared to the positive control, mice dosed with DOP showed increases in endurance, body weight, and food intake. Furthermore, DOP-feeding mice significantly increased the cell variability of T lymphocytes and B lymphocytes, compared with that of mice in control group. This study indicates that the unique and dominant polysaccharide DOP of D. officinale has stronger anti-fatigue activity than Rhodiola rosea extract. As such, DOP has promising potential for pharmaceutical development into health products to reduce fatigue.

show abstract

“…Dendrobium officinale (Chinese name: Tie-pi-shi-hu ) is an edible perennial orchid plant that has commonly been employed as a functional food in China and other Asian countries for thousands of years [ 1 ]. D. officinale is frequently infused in juice, tea and wine as a functional liquid, but can also be chewed directly or stewed in soup, porridge and other dishes as a high-quality agricultural vegetable in dietary supplementation [ 2 ], and has been approved by the China Food and Drug Administration ( ). Phytochemical analyses of stems in D. officinale have validated the presence of antioxidants [ 3 ], which are associated with anti-angiogenesis [ 4 ], immuno-stimulating activity [ 5 ] and the attenuation of ulcerative colitis [ 6 ], biofunctional properties that are due to large amounts of polysaccharides, flavonoid, phenanthrenes, bibenzyls and polyphenolic compounds [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Accumulation of Anthocyanins in Dendrobium officinale Stems with Red and Green Peels

Liao

Silva³

et al. 2018

IJMS

View full text Add to dashboard Cite

Dendrobium officinale stems, including red and green stems, are widely used as a dietary supplement to develop nutraceutical beverages and food products. However, there is no detailed information on pigment composition of red and green stems. Here, we investigated the content and composition of pigments in red and green stems by Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry and assessed the differential accumulation of anthocyanins at the molecular level. The color of peels in red stems was caused by the presence of anthocyanins in epidermal cells unlike the peels of green stems. The glucoside derivatives delphinidin and cyanidin are responsible for the red color. Within the D. officinale anthocyanidin biosynthetic pathway, DoANS and DoUFGT, coding for anthocyanidin synthase and UDP-glucose flavonoid-3-O-glucosyltransferase, respectively, are critical regulatory genes related to the differential accumulation of anthocyanidin. These findings provide a more complete profile of pigments, especially anthocyanin, in D. officinale stems, and lay a foundation for producing functional foods.

show abstract

Structure Characterization and Immunomodulating Effects of Polysaccharides Isolated from Dendrobium officinale

Cited by 134 publications

References 43 publications

Purification, Preliminary Characterization, and Immunological Activity of Polysaccharides from Crude Drugs of Sijunzi Formula

Purification, Preliminary Characterization, and Immunological Activity of Polysaccharides from Crude Drugs of Sijunzi Formula

Anti-Fatigue Effects of the Unique Polysaccharide Marker of Dendrobium officinale on BALB/c Mice

Differential Accumulation of Anthocyanins in Dendrobium officinale Stems with Red and Green Peels

Contact Info

Product

Resources

About