Structure characterization and antioxidant activity analysis of polysaccharides from Lanzhou Lily

Abstract

Lanzhou Lily (Lilium davidii var. unicolor) is a traditional medicinal plant and popular edible vegetable bulb in China. In this study, the polysaccharides of Lanzhou Lily (LLPs) were extracted by polyethylene glycol-based ultrasonic-assisted enzymatic extraction method (PEG-UAEE). The optimum process conditions were obtained by single-factor experiments and response surface methodology (RSM). Then, the preliminarily structure of LLPs was characterized by HPLC, FT-IR, and SEM, and its antioxidant activities were evaluated. The results showed that LLPs yield reached 14.75% under the optimized conditions: E/S ratio 1,400 U/g; pH 5.0, ultrasonic time 30 min; and ultrasonic temperature 50 °C. The LLPs has pyranoid ring, uronic acid, and the characteristic absorption peaks of -OH, C = O, and C-H. The results of scanning electron microscope indicated that the LLPs had irregular distribution, dispersed structure, and many holes. The HPLC analysis showed that the LLPs were heteropolysaccharide containing galactose (6.36%), glucose (76.03%), rhamnose (2.02%), and arabinose (7.09%). Moreover, the LLPs showed obvious antioxidant effect in vitro.

Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide

“…The crude SLMPs were extracted by PEG-UAEE method, alcohol precipitation, deproteinization, and freeze-drying with a yield of 10.95 ± 0.13%, which was calculated using the weight of the dried SLM leaves. Since PEG can provide more -OH groups, it can enhance the solubility of polysaccharides in water and thus increase the yield of polysaccharides ( 1 ).…”

“…Polysaccharides, a kind of natural macromolecular polymers, have been reported to have various biological activities, including antioxidant ( 1 ), anti-tumor ( 2 ) and enhancing immune activity ( 3 ), etc. In recent years, many natural plant polysaccharides have emerged as one of the hot topics and are widely perceived as ideal candidates for immunomodulatory agents in functional food ( 4 ) and practical medical fields due to their relatively low side effects and toxicity ( 5 ).…”

“…Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide Xuhua Yang 1 , Honghai Liu 2 , Jutian Yang 1,3 , Zhongren Ma 1 , Penghui Guo 1,3 , Hong Chen 1 and Dandan Gao 1,3 * Introduction Polysaccharides, a kind of natural macromolecular polymers, have been reported to have various biological activities, including antioxidant (1), anti-tumor (2) and enhancing immune activity (3), etc. In recent years, many natural plant polysaccharides have emerged as one of the hot topics and are widely perceived as ideal candidates for immunomodulatory agents in functional food (4) and practical medical fields due to their relatively low side effects and toxicity (5).…”

Network pharmacological analysis for the identification of the molecular mechanism ofLilium brownii(Baihe) against gastric cancer: 3-Demethylcolchicine targeting androgen receptor

“…These compounds have antitumor, antidepressant, antioxidant, antiinflammatory, immunomodulatory, hypoglycemic, and antifungal effects 3,[13][14][15] . They include steroidal saponins, polysaccharides, and phenolics, which may have antitumor effects by interrupting the cycle, triggering apoptosis, and stimulating the immune system 14,16 . However, the therapeutic effects and potential mechanisms of Baihe for GC are still unclear.…”

Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis

“…Recent studies have demonstrated that plant polysaccharides play a vital role in plant growth and development and have a wide range of antioxidant activities [11][12][13][14]. Polysaccharides, the major active components of A. continentalis, are mostly present in water extracts.…”