Structure, cell-specific expression, and mating-induced regulation of a Drosophila melanogaster male accessory gland gene

DiBenedetto, Angela J.; Harada, Heidi A.; Wolfner, Mariana F.

doi:10.1016/0012-1606(90)90284-p

Cited by 67 publications

(39 citation statements)

References 46 publications

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“…versus deletion, 0.07G0.009 s.e.). Expression level of PEBII measured in virgin males (Fiumera et al 2005) was not associated with cost of mating ( pZ0.28), but since Acps are known to be upregulated post-mating (DiBenedetto et al 1990;Betram et al 1992), it would be valuable to investigate the relationship between cost of mating and expression of Acps in non-virgin males. We also did not identify any polymorphisms in the nearby mating plug gene, PEBme, that were associated with female mortality (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Natural variation in male-induced ‘cost-of-mating’ and allele-specific association with male reproductive genes in Drosophila melanogaster

Fiumera

Dumont

Clark

2006

Phil. Trans. R. Soc. B

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One of the most sharply defined sexual conflicts arises when the act of mating is accompanied by an inflated risk of death. Several reports have documented an increased death rate of female Drosophila as a result of recurrent mating. Transgenic and mutation experiments have further identified components of seminal fluid that are at least in part responsible for this toxicity. Variation among males in their tendency for matings to be toxic to their partners has also been documented, but here for the first time we identify polymorphism within particular genes conferring differential postmating female mortality. Such polymorphism is important, as it raises the challenge of whether sexual conflict models can provide means for maintenance of polymorphism. Using a set of second chromosome extraction lines, we scored differences in post-mating female fecundity and longevity subsequent to mating, and identified significant among-line differences. Seventy polymorphisms in ten male reproductive genes were scored and permutation tests were used to identify significant associations between genotype and phenotype. One polymorphism upstream of PEBII and an amino acid substitution in CG17331 were both associated with male-induced female mortality. The same allele of CG17331 that is toxic to females also induces greater refractoriness to remating in the females, providing an example of an allele-specific sexual conflict. Postcopulatory sexual selection could lead to sexual conflict by favouring males that prevent their mates from mating, even when there is a viability cost to those females.

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Section: Resultsmentioning

confidence: 99%

Natural variation in male-induced ‘cost-of-mating’ and allele-specific association with male reproductive genes in Drosophila melanogaster

Fiumera

Dumont

Clark

2006

Phil. Trans. R. Soc. B

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“…To examine main-cell gene expression, the reporter construct was Acp9SEF-lacZ (15) (15). We were concerned that there might be very low levels of expression by this regulatory region, below the sensitivity of 3galactosidase assays.…”

Section: Methodsmentioning

confidence: 99%

“…The mc/DTA-E fusion encoding full-strength DTA was constructed by replacing the 3.1-kb lacZ fragment of the Acp95EF-lacZ (originally called mst316-lacZ) construct described in ref. 15 with a 700-bp BamHI-Bgl II fragment containing the DTA gene from the DT-A cassette described in ref. 19.…”

Section: Methodsmentioning

confidence: 99%

“…Intracellular production of DTA has been used to kill cells in a variety of systems by inhibiting translation (18)(19)(20)(21). We fused sequences encoding DTA to sequences that promote expression beginning at or just before eclosion only in main cells (15). Females mated to transgenic flies carrying this fusion show reduced copulation effects on receptivity and oviposition, even in cases when they receive and store sperm.…”

mentioning

confidence: 99%

“…Each cell type expresses a unique set of genes (14)(15)(16)(17). For example, Acp95EF and sex peptide are synthesized only by the main cells (15,17).…”

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confidence: 99%

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Probing the function of Drosophila melanogaster accessory glands by directed cell ablation.

Kalb

DiBenedetto

Wolfner

1993

Proc. Natl. Acad. Sci. U.S.A.

208

227

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The female Drosophila melanogaster fly undergoes behavioral changes after mating, including an increase in egg laying and an avoidance of remating. Accessory-gland products elicit these changes transiently when introduced into unmated female flies. We report here the generation and phenotype of flies that lack functional accessory-gland main cells as a consequence of genetically directed delivery of diphtheria toxin subunit A to those ceUls. Only main-cell secretions are essential for the short-term inhibition to remating; no other products of the genital tract can replace their function. Long-term inhibition to remating depends only on the storage of sperm in the female. Both sperm and main-cell secretions have roles in the increase of egg laying by the mated female. In addition to full-strength diphtheria toxin, we used low-activity toxins to kill only those ceUls that express toxin at high levels. These transgenic strains that express diphtheria toxins of different strengths in accessory-gland main cels wiUl be useful in further defining the role of these cells.In insects, mating causes females to undergo physiological and behavioral changes that persist for several days (1, 2). After mating, females lay eggs at a high rate and they reject further male courtship and remating. They store and utilize sperm they receive. In Drosophila melanogaster, these behavioral changes are due in part to sperm and in part to seminal fluid (3-5). Seminal-fluid components have been shown to cause the initial decrease in receptivity, called the "copulation effect" (3), but the long-term decrease in receptivity, called the "sperm effect" (4, 5), is correlated with the presence of sperm in the female's sperm-storage organs.To determine which molecules are responsible for these behavioral changes, we need to know their source. Seminalfluid effects on oviposition (egg laying) rate and receptivity were demonstrated in Hihara's study (3) of mates of males that had depleted their seminal secretions due to multiple prior matings. Hihara (3) postulated that products of the accessory gland, a secretory tissue in the male reproductive tract, caused these behavioral changes. He reasoned this because the accessory glands of multiply mated males were shrunken, apparently due to depletion of their contents, and because transplantation ofaccessory glands (6, 7) or injection of their extracts (for review, see ref.2) was known to cause increases in oviposition and decreases in receptivity. Chen et al. (8) and Aigaki et al. (9) later showed that a single accessory-gland peptide, the sex peptide, is able to induce oviposition and rejection when introduced into female flies.

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Female sex pheromone suppression and the fate of sex‐peptide‐like peptides in mated moths of Helicoverpa armigera

Nagalakshmi

Applebaum

Azrielli

et al. 2007

Arch Insect Biochem Physiol

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Insect males produce accessory gland (MAG) factors that are transferred in the seminal fluid to females during copulation, and elicit changes in the mated female's behavior and physiology. Our previous studies showed that the injection of synthetic Drosophila melanogaster sex-peptide (DrmSP) into virgin females of the moth Helicoverpa armigera causes a significant inhibition of pheromone production. In this and other moth species, pheromone production, correlated with female receptivity, is under neuroendocrine control due to the circadian release of the neuropeptide PBAN. In this study, we show that PBAN, present in the hemolymph during the scotophase in females, is drastically reduced after mating. We also identify 4 DrmSP-like HPLC peaks (Peaks A, S1, S2, and B) in MAGs, with increasing levels of DrmSP immunoreactivity during the scotophase, when compared to their levels observed during the photophase. In H. armigera MAGs, a significant reduction in the pheromonostatic peak (Peak B) was already evident after 15 min of copulation, and depletion of an additional peak (Peak S2) was evident after complete mating. Peak A is also detected in female brains, increasing significantly 1 h after mating, at which time inhibition of pheromone biosynthesis also occurs. However, changes corresponding to the other MAG peaks were not detected in mated female tissues.

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Structure, cell-specific expression, and mating-induced regulation of a Drosophila melanogaster male accessory gland gene

Cited by 67 publications

References 46 publications

Natural variation in male-induced ‘cost-of-mating’ and allele-specific association with male reproductive genes in Drosophila melanogaster

Natural variation in male-induced ‘cost-of-mating’ and allele-specific association with male reproductive genes in Drosophila melanogaster

Probing the function of Drosophila melanogaster accessory glands by directed cell ablation.

Female sex pheromone suppression and the fate of sex‐peptide‐like peptides in mated moths of Helicoverpa armigera

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