Structure, behaviour and repetitive DNA of B-chromosomes in<i>Cestrum nocturnum</i>(Solanaceae)

Montechiari, Keisy Ayelén; González, María Laura; Santos, Anahí Mara Yañez; Rutz, Jésica Lidiana Hajduczyk; Urdampilleta, Juan Domingo

doi:10.1080/11263504.2018.1559249

Cited by 6 publications

(2 citation statements)

References 45 publications

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“…The estimate of the repetitive fraction using low coverage sequencing data of Cestrum elegans and C. strigilatum showed retrotransposons with LTR (LTR-RTs) as the most accumulated elements of repetitive fraction (~60%). Sequences were highly similar among these two genomes, and reports including FISH with LTR-RT probes always exhibited a scattered distribution along chromosomes [26,27]. Accumulation of retrotransposons in Cestrum seems to follow the general trend of plants [28].…”

Section: Discussionmentioning

confidence: 99%

Diversity of satDNA fraction in Cestrum, the Genus with the Largest Genomes within Solanaceae

Souza

Parteka

Assis

et al. 2021

Preprint

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Cestrum species present large genomes (~24 pg), a high occurrence of B chromosomes, and great diversity in heterochromatin bands. Despite this, there is maintenance of chromosome shape and karyotype symmetry. To deepen our knowledge on Cestrum genome composition, low coverage sequencing data of C. strigilatum and C. elegans were compared. Bioinformatics analyses showed retrotransposons comprising more than 70% of the repetitive fraction, followed by transposons (~18%). The four satDNA families that accumulated the most in the datasets were used as probes in FISH assays, and showed different distribution profiles along chromosomes. Most hybridization signals were located in the C-CMA/DAPI banding sites, including those related to AT-rich Cold-Sensitive Regions (CSRs) and heterochromatin. Although satellite probes hybridized in all tested species, a satDNA family named CsSat49 was highlighted as it predominates in centromeric regions. Data suggest that the satDNA fraction is still conserved in the genus, although there is variation in the number of FISH signals between karyotypes, as well as in the B chromosomes. This study brings an important advance in the knowledge on genome organization and heterochromatin composition in Cestrum, especially on the distribution and differentiation mechanisms of satellite fraction between species of a genus of Solanaceae with large genomes.

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Section: Discussionmentioning

confidence: 99%

Diversity of satDNA fraction in Cestrum, the Genus with the Largest Genomes within Solanaceae

Souza

Parteka

Assis

et al. 2021

Preprint

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“…CMA/DAPI technique helped to detect variability despite the morphological uniformity of the chromosomes, provided information on genetic variation at a population level regarding speciation, and revealed AT-and GC-rich regions of B chromosomes. Together with FISH (see below), this technique showed that B chromosomes possess nucleolar activity and nucleolar competition with the A chromosomes (Acosta and Moscone, 2011;Acosta et al, 2016;Montechiari et al, 2020).…”

Section: Chromosome Bandingmentioning

confidence: 99%

Chromosome Evolution in the Family Solanaceae

et al. 2022

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This review summarizes and discusses the knowledge of cytogenetics in Solanaceae, the tomato family, its current applications, and prospects for making progress in fundamental systematic botany and plant evolution. We compile information on basic chromosome features (number, size, morphology) and molecular cytogenetics (chromosome banding and rDNA patterns). These data were mapped onto the Solanaceae family tree to better visualize the changes in chromosome features and evaluate them in a phylogenetic context. We conclude that chromosomal features are important in understanding the evolution of the family, especially in delimiting clades, and therefore it is necessary to continue producing this type of data. The potential for future applications in plant biology is outlined. Finally, we provide insights into understanding the mechanisms underlying Solanaceae’s diversification that could substantially contribute to developing new approaches for future research.

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Genetic Diversity Assessment in Vegetable Crops

Shankar,

Kaushik

2024

Sustainable Development and Biodiversity

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Structure, behaviour and repetitive DNA of B-chromosomes inCestrum nocturnum(Solanaceae)

Cited by 6 publications

References 45 publications

Diversity of satDNA fraction in Cestrum, the Genus with the Largest Genomes within Solanaceae

Diversity of satDNA fraction in Cestrum, the Genus with the Largest Genomes within Solanaceae

Chromosome Evolution in the Family Solanaceae

Genetic Diversity Assessment in Vegetable Crops

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